Oxidative phosphorylation by subcellular particles from yeast

Role of bivalent cations in the control of enzymes involved in gluconeogenesis

“…Protein concentration wag measured by the method of Lowry et al [8]. The assay medium used was that described by Utter and Keech [9] and contained in final concentration 112 mM tris-HCl, ATP 2 mM, magnesium sulphate 8 mM, sodium bicarbonate 20 mM, sodium pyruvate 2mM, NADH 0.2 mM, acetylCoA 0.24 mM (prepared in situ from CoA, acetylphosphate and phosphotransacetylase [10]) and 2.2 units malate dehydrogenase; the final pH was 7.2 at 30 °. The reaction was initiated by the addition of 0.1 ml of the enzyme extract to 0.9 ml of assay medium and the rate of disappearance of NADH was followed for about 10 min.…”

Mutants ofAspergillus nidulans lacking pyruvate carboxylase

“…Omission of acetyl co-enzyme A caused little loss of activity, but since this compound may be present at significant concentrations in crude extracts it is not possible to deduce if acetyl-CoA is required for activation of the enzyme in A. nidulans as it is in most other organisms [14].…”

Die chemische struktur der biosynthetischen vorstufe des squalens

“…Zur Gewinnung der enzymatisch aktiven Partikelfraktion aus Hefe wurden die Zellen im Homogenisator nach Schlossmann [8] aufgeschlossen, zun/ichst 20 min bei 15 000 g zentrifugiert und aus dem Uberstand die aktive Fraktion durch 60 min Zentrifugieren bei 105 000 g sedimentiert [9]. Nach Vorversuchen, in denen wir die Zeit-und Enzymabh/ingigkeit der Bildung von PSPP bestimmten, erwies sich folgende Zusammensetzung fiir die Pr/iparation des PSPP als geeignet.…”