Oxidative post translational modifications of proteins related to cell cycle are involved in cadmium toxicity in wheat seedlings

Pena, Liliana B.; Barcia, Roberto A.; Azpilicueta, Claudia E.; Méndez, Andrea A.E.; Gallego, Susana M.

doi:10.1016/j.plantsci.2012.07.008

Cited by 79 publications

(45 citation statements)

References 45 publications

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“…During the symbiotic interaction between Medicago truncatula and Sinorhizobium meliloti multiple proteins involved in nitrogen fixation are sulfenylated, highlighting the importance of ROS in the establishment and development of this plant-biotic interaction (Oger et al, 2012). On the other hand, Triticum aestivum seeds germinated in the presence of cadmium and other oxidative stressors showed that ROS-induced PTM of proteins are involved in cell cycle progression during root growth, affecting G1/S transition and progression through the S phase (Pena et al, 2012).…”

Section: Post-translational Regulationmentioning

confidence: 99%

Reactive oxygen species and plant resistance to fungal pathogens

et al. 2015

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Reactive oxygen species (ROS) have been studied for their role in plant development as well as in plant immunity. ROS were consistently observed to accumulate in the plant after the perception of pathogens and microbes and over the years, ROS were postulated to be an integral part of the defence response of the plant. In this article we will focus on recent findings about ROS involved in the interaction of plants with pathogenic fungi. We will describe the ways to detect ROS, their modes of action and their importance in relation to resistance to fungal pathogens. In addition we include some results from works focussing on the fungal interactor and from studies investigating roots during pathogen attack.

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Section: Post-translational Regulationmentioning

confidence: 99%

Reactive oxygen species and plant resistance to fungal pathogens

et al. 2015

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“…Total purified RNA was reversely transcribed using RevertAid First Strand cDNA Synthesis Kit (Thermo, USA, K1066). Both the sequence of primers used and RT-PCR reaction performed in the experiment were according to Pena et al [3] and Ni et al [21] and are listed in Tab. 1.…”

Section: Rna Extraction and Rt-pcr Analysismentioning

confidence: 99%

“…The Fluo-3 can specifically combine with the Ca 2+ and gives a strong fluorescence Tab. 1 RT-PCR reaction system and amplification procedure [3]. mcm2 -minichromosomal maintenance factor; pcna -proliferating cell nuclear antigen; rdr -ribonucleotide reductase small subunit.…”

Section: Fluo-3/am Stainingmentioning

confidence: 99%

“…3). As mcm2, pcna, and rdr are potential E2F transcriptional targets and are regarded as cell-cycle gene markers important in regulation of the G1/S transition [3,[7][8][9][10][11][12], the downregulation of these marker genes implies that EGTA treatment interferes with G1/S transition of the cell cycle.…”

Section: Rt-pcr Analysismentioning

confidence: 99%

“…Cyclin D-CDK4/6 complexes phosphorylate the retinoblastoma protein (pRB) to dissociate it from E2F family of transcription factors, which is a crucial step in the coordination of the G1/S transition [2,3].…”

Section: Introductionmentioning

confidence: 99%

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EGTA, a calcium chelator, affects cell cycle and increases DNA methylation in root tips of Triticum aestivum L.

Zhang

Shi

et al. 2016

Acta Soc Bot Pol

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In this study, when germinated Triticum aestivum L. seeds were treated with 0, 2, 4 and 6 mM ethyl glycol tetraacetic acid (EGTA), root growth was suppressed and the mitotic index decreased. These inhibitory effects were positively correlated with EGTA concentration. RT-PCR analysis revealed that the expression of several gene markers related to the G1/S transition of the cell cycle were significantly downregulated. Confocal microscopy of Fluo-3/AM-stained roots showed chelation of nearly all of the Ca 2+ within the root meristematic regions. Both random amplified polymorphic DNA (RAPD) and coupled restriction enzyme digestion-random amplification (CRED-RA) techniques showed significant increases in the levels of genomic DNA polymorphisms and degree of DNA methylation. The study provides information concerning the impact of Ca 2+ chelator, EGTA, on the growth, expression of cell cycle transition marker genes, and changes in DNA structure and methylation in the wheat roots.

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