Oxidative preconditioning affords protection against carbon tetrachloride‐induced glycogen depletion and oxidative stress in rats

Abstract

The rectal insufflation of a judicious dose of ozone, selected from that used in clinical practice, is able to promote oxidative preconditioning or oxidative stress tolerance preventing the hepatocellular damage mediated by free radicals. In order to evaluate the effects of ozone oxidative preconditioning on carbon tetrachloride-mediated hepatotoxicity, the following experimental protocol was designed: group 1 (negative control, sunflower oil i.p.); group 2 (CCl(4) in sunflower oil, 1 ml kg(-1) i.p.); group 3 (15 ozone-oxygen pretreatments at a dose of 1 mg kg(-1) via rectal insufflation + CCl(4) as in group 2); group 4 (ozone control group, 15 ozone-oxygen pretreatments + sunflower oil i.p.). Ozone pretreatment prevented glycogen depletion (as demonstrated by biochemical and histopathological findings) and avoided lactate overproduction associated with the hepatotoxic effects of CCl(4). The administration of CCl(4) increased lipid peroxidation (as measured by thiobarbituric acid-reactive substances) and uric acid levels and inhibited superoxide dismutase activity. All these deleterious effects induced by CCl(4) were prevented by ozone pretreatment. The administration of ozone without CCl(4) (ozone control group) did not produce any changes in the evaluated parameters. Our results showed that ozone treatment, in our experimental conditions, was able to prevent anaerobic glycolysis and oxidative stress induced by CCl(4).

Preconditioning with ozone/oxygen mixture induces reversion of some indicators of oxidative stress and prevents organic damage in rats with fecal peritonitis

“…Recently, it was reported that OOP is able to afford protection against injury associated with hepatic ischemiareperfusion and cellular damage mediated by free radicals [19][20][21][22]. These authors have provided evidence that ozone may induce an adaptation to oxidative stress and also prepare the host to face physiopathological conditions mediated by ROS.…”

Acute oxygen-ozone administration to rats protects the heart from ischemia reperfusion infarct

“…Ozone and myocardial injury in rats 449 increased antioxidant endogenous systems involving glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT), thus preparing the host to face physiopathological conditions mediated by ROS [4,7,15,[17][18]. It is also supported by the fact that ozone, probably by means of an oxidative preconditioning mechanism, similar to ischemic preconditioning, protects these organs from the damage produced by ROS, which induces improvement of antioxidantpro-oxidant balance and the concomitant preservation of cell redox state [5].…”

Effects of l-Canavanine and ozone on vascular reactivity in septicemic rats

“…In addition, it has been reported that O 3 could stimulate and or preserve endogenous hepatic antioxidant enzymes activity after hepatic reperfusion injury [40] and work done by Alvarez et al [2] documented that this may occur through different mechanisms such as oxidative preconditioning mechanism and/or by blocking the xanthine/xanthine oxidase pathway for ROS generation with subsequent adenosine accumulation and decrease ROS generation. Candelario-Jalil et al [11] suggested that low doses of O 3 may increase the antioxidant endogenous systems including GSH-Px, SOD and CAT, preparing the host to face physiopathological conditions mediated by ROS. This is in accordance with our work, where we showed significant enhancement of hepatic antioxidant enzyme activity SOD and GSH-Px.…”

“…Septicemia not only leads to overproduction of reactive-oxygen species (ROS) but also consumption of endogenous antioxidant enzymes with associated inhibition of the hepatic antioxidant enzyme synthesis such as superoxide dismutase SOD [11] and glutathione peroxidase (GSH-Px) [30].…”