Oxidative Status and Plasma Lipid Profile in β-Thalassemia Patients

Abstract

β-Thalassemia (β-thal) is a genetic disorder, representing a major health problem in Algeria. It is associated with altered lipid levels and a state of oxidative stress that can lead to cardiac complications and premature death. We examined the plasma lipid profile and redox status of 46 patients with β-thal major (β-TM) and β-thal intermedia (β-TI) compared to 36 healthy subjects. Plasma lipids including total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) were investigated. Oxidative status was evaluated by measuring malondialdehyde (MDA), reduced glutathione (GSH) and catalase (CAT) activity. The potential relationships between these parameters and the hemoglobin (Hb) blood concentrations, serum ferritin, duration and frequency of transfusion, splenectomy as well as age, were examined. Our data indicated that the study patients were under increased state of oxidative stress associated with hypertriglyceridemia, and hypocholesterolemia. The CAT activity was negatively correlated with Hb concentration and LDL-C/TG ratio and positively with years of transfusion. The elevated TC/HDL-C ratio particularly in β-TM patients who were younger, correlated positively with ferritinemia and triglyceride levels and suggested an increased coronary risk. This heightened risk state should lead to the inclusion of this index (TC/HDL-C) in clinical management, particularly in splenectomized patients.

Global analysis of erythroid cells redox status reveals the involvement of Prdx1 and Prdx2 in the severity of beta thalassemia

“…Several studies have already described the generation of ROS in erythrocytes and their significance in the physiopathology of beta-thalassemia. Some of them have shown that this increase is higher in BTM than BTI, both in plasma and erythrocytes [46, 47]. Besides corroborating these findings, our results also described increased levels of ROS in mononuclear cells and neutrophils of these patients, indicating that oxidative damages are not restricted to erythroid cells (Fig 1).…”

Differentially expressed plasma proteins of β-thalassemia/hemoglobin E patients in response to curcuminoids/vitamin E antioxidant cocktails

“…The abnormality of plasma lipid profile in thalassemic patients including decreased levels of total cholesterol, high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C), together with increased levels of triglycerides have been reported [26][27][28]. Our results showed the altered levels of apolipoprotein A-I, A-IV, C-II, and E in response to the treatments.…”

Erythrocyte Catalase Activity in More Frequent Microcytic Hypochromic Anemia: Beta-Thalassemia Trait and Iron Deficiency Anemia

“…They concluded that β -thalassemia minor RBCs react to increased OxS rising activities of antioxidant enzymes, while in β -thalassemia major normal antioxidant enzyme levels are due to the presence of normal RBCs from multiple blood transfusions. However, Boudrahem-Addour et al [ 27 ] observed a significant increase ( p < 0,05) of catalase activity in β -thalassemia major and intermedia. The present results, in concordance with Gerli et al [ 26 ], showed that some BTT subjects had increased catalase activity.…”