Oxidative Status Imbalance in Patients with Metabolic Syndrome: Role of the Myeloperoxidase/Hydrogen Peroxide Axis

Fonseca, Liliana; Nunes-Souza, Valéria; Guedes, Glaucevane da Silva; Schettino-Silva, Glauber; Mota‐Gomes, Marco A.; Rabelo, Luíza Antas

doi:10.1155/2014/898501

Cited by 19 publications

(15 citation statements)

References 56 publications

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“…Under conditions of chronic inflammation, HDL is oxidatively modified in the artery intima by the macrophage-derived enzyme MPO [ 42 ]. On HDL, apoA-I is the primary target of oxidation by MPO [ 43 ], and MPO activity is increased in subjects with MetSyn [ 44 ]. Oxidized apoA-I exhibits an irreversible loss of HDL exchangeability [ 27 , 31 ], and reduction in apoA-I’s HDL exchange kinetics results in reduced CEC [ 27 , 45 ].…”

Section: Discussionmentioning

confidence: 99%

Apolipoprotein A-I exchange is impaired in metabolic syndrome patients asymptomatic for diabetes and cardiovascular disease

Borja¹,

Hammerson²,

Tang

et al. 2017

PLoS ONE

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ObjectiveWe tested the hypothesis that HDL-apolipoprotein A-I exchange (HAE), a measure of high-density lipoprotein (HDL) function and a key step in reverse cholesterol transport (RCT), is impaired in metabolic syndrome (MetSyn) patients who are asymptomatic for diabetes and cardiovascular disease. We also compared HAE with cell-based cholesterol efflux capacity (CEC) to address previous reports that CEC is enhanced in MetSyn populations.MethodsHAE and ABCA1-specific CEC were measured as tests of HDL function in 60 MetSyn patients and 14 normolipidemic control subjects. Predictors of HAE and CEC were evaluated with multiple linear regression modeling using clinical markers of MetSyn and CVD risk.ResultsHAE was significantly reduced in MetSyn patients (49.0 ± 10.9% vs. 61.2 ± 6.1%, P < 0.0001), as was ABCA1-specific CEC (10.1 ± 1.6% vs. 12.3 ± 2.0%, P < 0.002). Multiple linear regression analysis identified apoA-I concentration as a significant positive predictor of HAE, and MetSyn patients had significantly lower HAE per mg/dL of apoA-I (P = 0.004). MetSyn status was a negative predictor of CEC, but triglyceride (TG) was a positive predictor of CEC, with MetSyn patients having higher CEC per mg/dL of TG, but lower overall CEC compared to controls.ConclusionsMetSyn patients have impaired HAE that contributes to reduced capacity for ABCA1-mediated CEC. MetSyn status is inversely correlated with CEC but positively correlated with TG, which explains the contradictory results from earlier MetSyn studies focused on CEC. HAE and CEC are inhibited in MetSyn patients over a broad range of absolute apoA-I and HDL particle levels, supporting the observation that this patient population bears significant residual cardiovascular disease risk.

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Section: Discussionmentioning

confidence: 99%

Apolipoprotein A-I exchange is impaired in metabolic syndrome patients asymptomatic for diabetes and cardiovascular disease

Borja¹,

Hammerson²,

Tang

et al. 2017

PLoS ONE

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“…The NEFA were measured in the medium in 0, 90, and 180 minutes of incubation and normalized by the amount of fat used for stimulation. [23] with modifications, as previously described [24]. Data were normalized per total protein concentration, measured by Bradford [21] and expressed as nM•mg protein -1 .…”

Section: Lipolysismentioning

confidence: 99%

“…Data were normalized per total protein concentration, measured by Bradford [21] and expressed as nM•mg protein -1 . Hydrogen peroxide (H 2 O 2 ) was measured by fluorescence using the Amplex® UltraRed hydrogen peroxide (10-acetyl-3,7-dihydroxiphenoxazine) assay (Invitrogen®, Paisley, United Kingdom) as described [24].…”

Section: Lipolysismentioning

confidence: 99%

3-Amino-1,2,4-Triazole Induces Quick and Strong Fat Loss in Mice with High Fat-Induced Metabolic Syndrome

Nunes-Souza

Dias-Júnior

Eleutério-Silva

et al. 2020

Oxidative Medicine and Cellular Longevity

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Background. Obesity is a growing epidemic with limited effective treatments and an important risk factor for several diseases such as metabolic syndrome (MetS). In this study, we aimed to investigate the effect of 3-amino-1,2,4-triazole (ATZ), an inhibitor of catalase and heme synthesis, in a murine model for MetS. Methods. Male C57BL/6 mice with high-fat diet-induced MetS received ATZ (500 mg·kg-1·24 h-1) for 12 weeks. Results. The HFD group showed increased blood pressure and body weight, enhanced fat deposition accompanied by an increase in adipocyte diameter, and decreased lipolysis in white adipose tissue (WAT). The expression of genes related to inflammation was increased in WAT of the HFD group. Concurrently, these mice exhibited an increase in leptin, nonesterified fatty acid (NEFA), insulin, and glucose in plasma, coupled with glucose intolerance and insulin resistance. Strikingly, ATZ prevented the increase in blood pressure and the HFD-induced obesity as observed by lower body weight, WAT index, triglycerides, NEFA, and leptin in plasma. ATZ treatment also prevented the HFD-induced increase in adipocyte diameter and even induced marked atrophy and the accumulation of macrophages in this tissue. ATZ treatment also improved glucose metabolism by increasing glucose tolerance and insulin sensitivity, GLUT4 mRNA expression in WAT in parallel to decreased insulin levels. Conclusions. In the context of HFD-induced obesity and metabolic syndrome, the fat loss induced by ATZ is probably due to heme synthesis inhibition, which blocks adipogenesis by probably decreased RevErbα activity, leading to apoptosis of adipocytes and the recruitment of macrophages. As a consequence of fat loss, ATZ elicits a beneficial systemic antiobesity effect and improves the metabolic status.

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“…The ability of SOD to compete in superoxide anions was revealed by the stage of nitro blue tetrazolium's inhibition of restoration up to hydrazine tetrazolium [28]. iii) Proteolytic enzymes and their inhibitors activity were studied with trypsin-like activity (TLA), elastase-like activity (ELA), alfa-1-antitripsin activity (ATA) and acid-stable inhibitors activity (ASA) by enzymatic methods [29]. iv) The level of serum protein was measured by Loury.…”

Section: Biochemical Assaysmentioning

confidence: 99%

Biological effects of grape polyphenols processing products in experimental metabolic syndrome

Kubyshkin¹,

Фомочкина²,

Ogai³

et al. 2018

Russ Open Med J

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Objective -Biological effects of grape processing products (GPP) rich in polyphenols are still not studied completely. The aim of the investigation was to study co-interactions between peroxidation lipid oxidation (PLO) and nonspecific proteases and their inhibitors activity in the animals with the model of metabolic syndrome (MS) corrected with GPP.Material and Methods -The study was performed on 54 white male rats of the weight of 180-200 g. The animals of experimental groups were fed by the standard menu and were given 10% fructose solution instead the drinking water during 8 weeks, which promoted development of MS. "Enoant", "Enoant-premium" and "Fenocor" were used as GPP in dosage of 2.5 ml/kg during 4 weeks just after 5 th week. The PLO was estimated according to the concentration of thiobarbituric acid active products (TBA-AP), ceruloplasmin (CC), catalaselike activity (CLA), peroxidase-like activity (PLA) and superoxide dismutase activity (SOD). Trypsin-like activity (TLA), elastase-like activity (ELA), alfa-1-antitripsin activity (ATA) and acid-stable inhibitors activity (ASA) were estimated. Results -MS demonstrated rise of TBA-AP by 50.4 % (р<0.01) and TLA by 19.5% (р<0.01), and drop of ATA, SOD, CLA, PLA in comparison with the control. The GPP (especially "Fenocor") led to the block of pathogenetic link of MS because of suppression of PLO and proteases' activity and increase of their inhibitors. Conclusion -There is a tight connection between PLO and proteases' activity in MS. "Fenocor" could be used as MS-modified remedies acting positively on key links of MS.

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Oxidative Status Imbalance in Patients with Metabolic Syndrome: Role of the Myeloperoxidase/Hydrogen Peroxide Axis

Cited by 19 publications

References 56 publications

Apolipoprotein A-I exchange is impaired in metabolic syndrome patients asymptomatic for diabetes and cardiovascular disease

Apolipoprotein A-I exchange is impaired in metabolic syndrome patients asymptomatic for diabetes and cardiovascular disease

3-Amino-1,2,4-Triazole Induces Quick and Strong Fat Loss in Mice with High Fat-Induced Metabolic Syndrome

Biological effects of grape polyphenols processing products in experimental metabolic syndrome

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