Oxidative status in different settings and with different methodological approaches compared by Receiver Operating Characteristic curve analysis

Abstract

This study reveals T-MDA as the best marker to detect oxidative stress, shows the ability of d-ROMs to identify modified oxidative status particularly in the presence of high damages, and evidences the poor TAC performance. d-ROMs and TAC assays could be useful for routine purposes; however, for an accurate clinical data evaluation, their comparison versus a "gold standard method" is required.

Prognostic relevance of oxidative stress measurement in chronic lymphocytic leukaemia

“…Based on our previous experience, in order to evaluate the OS we chose d‐ROMs test and BAP test as suitable tests to measure total oxidant and total antioxidant capacity, respectively, on blood serum samples . This choice was dictated by the well‐established analytical performances of these tests that allow them (i) to accurately measure the OS level ex vivo either in subject at risk for OS (eg cigarette smokers and alcohol drinkers) or in patients suffering from OS‐related diseases, including cardiovascular and inflammatory diseases, cancer and onco‐haematological disorders; (ii) to be sensitive to changes occurring after specific treatments, antioxidant supplementation and radio‐ or chemotherapy; (iii) to anticipate the progression of OS‐related diseases during a systematic monitoring; and (iv) to be minimally invasive, highly compliant, fast, with an optimal cost/benefit ratio . Both d‐ROMs test and BAP test have been validated by the gold standard methods to assess redox balance ex vivo (ie the electron spin magnetic resonance spectrometry), and their reference values in large cohorts of general population are well established .…”

“…d‐ROMs test is a photometric test that measures the total oxidant capacity of a blood serum/plasma sample against the N,N‐diethylparaphenylenediamine, used as chromogenic substrate . As previously described, both serum (20 μL) and chromogenic substrate (20 μL) were dissolved in a buffered acidic solution (until 1 mL) and incubated at 37°C; the progressive oxidation of the chromogenic substrate that reflects the total oxidant capacity of the serum sample was monitored through the absorbance change at 505 nm over the time, according to a kinetic procedure; the results that were compared to those of a control serum with known values were expressed in arbitrary units. Normal range is from 250 to 300 CARR U (corresponding to hydrogen peroxide 20‐24 mg/mL).…”

Pre‐diagnostic derivatives of reactive oxygen metabolites and the occurrence of lung, colorectal, breast and prostate cancer: An individual participant data meta‐analysis of two large population‐based studies

“…The sensitivity and specificity of the assay were 72.8% and 100%, respectively, when compared to total malondialdehyde as the reference index for lipid peroxidation. 15 Serum samples from the two cohorts were delivered to the Laboratory for Health Protection Research (Bilthoven, the Netherlands) where the d-ROM assay was adapted to an automatic analyzer (LX20-Pro, Beckman-Coulter, Woerden, the Netherlands), as described previously. 16,17 Inter-assay coefficients of variation were 3.7% and 2.2% in the ESTHER study and Tromsø study, respectively.…”

The role of oxidative stress in α-amanitin-induced hepatotoxicityin an experimental mouse model

“…In recent studies on oxidative stress, total oxidant status (TOS) and total antioxidant status (TAS) have been measured, in addition to the evaluation of the enzymatic activities separately. Measuring TAS and TOS levels provides valuable information regarding the net oxidative stress the organism suffers (19)(20)(21)(22). In the available literature, there is no study on α-amanitin poisoning in which TOS and TAS levels are evaluated.…”