Oxidative Stress and Antioxidant Status in Mouse Liver: Effects of Dietary Lipid, Vitamin E and Iron ,

Ibrahim, Wissam H.; Lee, Ung‐Soo; Yeh, Che‐Chung; Szabö, Joseph; Bruckner, Géza; Chow, Ching K.

doi:10.1093/jn/127.7.1401

Cited by 119 publications

(68 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Since IL-4 is known to suppress the production of other reactive nitrogen and oxygen species in addition to NO (32)(33)(34)(35)(36), and these intermediates may also be involved in mediating liver pathology (37)(38)(39), the generation of O 2 Ϫ , hydrogen peroxide, and ONOO Ϫ by granuloma cells was investigated. To examine O 2 Ϫ production, granuloma cells were stimulated with PMA, and O 2 Ϫ was detected by measuring the formation of blue formazan after addition of NBT.…”

Section: Reactive Oxygen and Nitrogen Intermediates (Roi/rni) Productmentioning

confidence: 99%

IL-4 Plays a Crucial Role in Regulating Oxidative Damage in the Liver During Schistosomiasis

Flamme

Patton

Bauman

et al. 2001

The Journal of Immunology

View full text Add to dashboard Cite

Liver enlargement and hepatocyte proliferation, normal responses in wild-type (WT) mice infected with the parasitic helminth Schistosoma mansoni, were found to be severely impaired in infected IL-4−/− mice. Compared with WT mice, increased levels of O2−, NO, and the more highly reactive ONOO− were detected in the liver and produced by lesional cells isolated from liver granulomas of infected IL-4−/− mice. Concurrently, antioxidant defenses in the liver, specifically catalase levels, diminished dramatically during the course of infection in these animals. This contrasted to the situation in infected WT mice, where catalase levels remained as high as those in normal mice. Actual levels of reactive oxygen and nitrogen intermediates in the livers of infected IL-4−/− animals are thus likely to be considerably higher than those in the livers of infected WT mice. To determine whether these changes contributed to the development of the more severe disease that characterizes infection in the IL-4−/− animals, we treated infected IL-4−/− mice with uric acid, a potent scavenger of ONOO−. This resulted in significantly increased hepatocyte proliferation, decreased morbidity, and prolonged survival. Taken together, these data indicate that IL-4 is playing a protective role during schistosomiasis by controlling the tight regulation of the generation of reactive oxygen and nitrogen intermediates in the liver.

show abstract

Section: Reactive Oxygen and Nitrogen Intermediates (Roi/rni) Productmentioning

confidence: 99%

IL-4 Plays a Crucial Role in Regulating Oxidative Damage in the Liver During Schistosomiasis

Flamme

Patton

Bauman

et al. 2001

The Journal of Immunology

View full text Add to dashboard Cite

show abstract

“…eicosapentaenoic acid (EPA), 20 : 5(n-3) and docosahexaenoic acid (DHA), 22 : 6(n-3)) of fish oils are extremely susceptible to lipid peroxidation, even in the presence of added dietary antioxidants (Gonzalez et al, 1992). The data evaluating the effect of fish oil n-3 PUFA on oxidative stress and lipid peroxidation markers in vivo vary with both animal and clinical studies reporting increased oxidation (Ibrahim et al, 1997), no effect on oxidation (Nenseter et al, 1992) and even some indication of decreased susceptibility to induced oxidation (Yuan and Kitts, 2002).…”

mentioning

confidence: 99%

Ovarian follicular development, lipid peroxidation, antioxidative status and immune response in laying hens fed fish oil-supplemented diets to produce n-3-enriched eggs

Ebeid

Eid

Saleh

et al. 2008

Animal

View full text Add to dashboard Cite

The objective of the present study was to research the effect of feeding laying hens fish oil-supplemented diets to produce n-3-enriched eggs on their ovarian follicular development, serum lipid peroxidation, antioxidative status and immune response. A total of 105 white Bovens hens at 24 weeks of age were housed in cages in an open-sided building under a 16 h light : 8 h dark lighting schedule. Birds were randomly divided into five treatments and were fed, ad libitum, diets containing 0% (control), 1.25%, 2.5%, 3.5% or 5.0% fish oil from 24 to 36 weeks of age. Egg production and weight were recorded. By weeks 35 and 36 of age 15 eggs were taken at random from each treatment to determine the yolk lipid profile and cholesterol content. At the end of the experimental period, 10 females from each treatment were randomly chosen, anaesthetised and killed by decapitation. Ovary and oviduct samples were immediately weighted and ovarian follicles were classified. Serum thiobarbituric acid-reactive substance (TBARS), hepatic TBARS and hepatic glutathione peroxidase (GSH-Px) activity were measured. No clear trend was observed concerning egg production and egg yolk cholesterol. As dietary fish oil levels increased, n-3-polyunsaturated fatty acids (n-3 PUFA) increased, whereas n-6 PUFA tended to decrease in yolk lipids. No negative effects were detected in ovary and oviduct weights, expressed in both absolute terms and relative to body weight. The numbers and total weights of large yellow follicles (LYF) in the ovary were not significantly affected by fish oil supplementation. Low levels (1.25% to 2.5%) of fish oil reduced both plasma and hepatic TBARS and enhanced GSH-Px activity. It is also interesting to note that inclusion of 2.5% fish oil in laying hen diets enhanced the antibody titre in laying hens. Therefore, it could be concluded that inclusion of fish oil in laying hen diets at moderate levels increased the n-3 fatty acids content in eggs, improved antioxidative status, enhanced the antibody response and did not have a negative influence on the different reproductive morphology parameters in laying hens.

show abstract

“…Fish are the major source of EPA ϩ DHA, but fish oil supplements produce a decreased oxidative stability of plasma LDL (15,16). In contrast, consumption of fish itself may be protective against type 2 diabetes (17).…”

mentioning

confidence: 99%

Including Walnuts in a Low-Fat/Modified-Fat Diet Improves HDL Cholesterol-to-Total Cholesterol Ratios in Patients With Type 2 Diabetes

et al. 2004

View full text Add to dashboard Cite

OBJECTIVE -The aim of this study was to examine the effect of a moderate-fat diet inclusive of walnuts on blood lipid profiles in patients with type 2 diabetes.RESEARCH DESIGN AND METHODS -This was a parallel randomized controlled trial comparing three dietary advice groups each with 30% energy as fat: low fat, modified low fat, and modified low fat inclusive of 30 g of walnuts per day. Fifty-eight men and women, mean age 59.3 Ϯ 8.1 years, started the trial. Dietary advice was given at baseline with monthly follow-up and fortnightly phone calls for support. Body weight, percent body fat, blood lipids, HbA 1c , total antioxidant capacity, and erythrocyte fatty acid levels were measured at 0, 3, and 6 months. Data were assessed by repeated-measures ANOVA with an intention-to-treat model. RESULTS -The walnut group achieved a significantly greater increase in HDL cholesterolto-total cholesterol ratio (P ϭ 0.049) and HDL (P ϭ 0.046) than the two other treatment groups. A 10% reduction in LDL cholesterol was also achieved in the walnut group, reflecting a significant effect by group (P ϭ 0.032) and time (P ϭ 0.036). There were no significant differences between groups for changes in body weight, percent body fat, total antioxidant capacity, or HbA 1c levels. The higher dietary polyunsaturated fat-to-saturated fat ratio and intakes of -3 fatty acids in the walnut group were confirmed by erythrocyte biomarkers of dietary intake.CONCLUSIONS -Structured "whole of diet" advice that included 30 g of walnuts/day delivering substantial amounts of polyunsaturated fatty acid improved the lipid profile of patients with type 2 diabetes. The literature on recommendations varies to a minor degree, and cultural differences may have an influence (9), but a total fat level of Յ30% energy (10) and Ͻ10% saturated fat is reasonably well accepted (11,12). There is some concern that PUFAs are more susceptible to oxidation and therefore may be more atherogenic (11), so a level of Ͻ10% energy for PUFAs is seen with greater flexibility in the proportions of carbohydrate and MUFA (13). Within the PUFA fraction of the diet, the ratio of -6 to -3 fatty acids is also considered (9) with daily recommendations for a 2,000-kcal diet of 0.65 g for the long-chain -3s (EPA ϩ DHA) and 2.22 g for ALA (14) and the -6 -to--3 ratio reduced to substantially Ͻ10 (14a). Diabetes CareFish are the major source of EPA ϩ DHA, but fish oil supplements produce a decreased oxidative stability of plasma LDL (15,16). In contrast, consumption of fish itself may be protective against type 2 diabetes (17). Nuts provide substantial amounts of dietary PUFA, and nut consumption is inversely associated with the risk of type 2 diabetes in women (18). There is no net effect on glucose homeostasis from nut supplementation, but more interestingly, no net weight gain when nuts were used as a replacement food (19). In subjects with dyslipidemia (total cholesterol Ͼ5.17; LDL Ͼ3.36; triglycerides Ͼ2.26 mmol/l), a low-fat diet supplemented with walnuts was found to reduce total choles...

show abstract

Oxidative Stress and Antioxidant Status in Mouse Liver: Effects of Dietary Lipid, Vitamin E and Iron ,

Cited by 119 publications

References 34 publications

IL-4 Plays a Crucial Role in Regulating Oxidative Damage in the Liver During Schistosomiasis

IL-4 Plays a Crucial Role in Regulating Oxidative Damage in the Liver During Schistosomiasis

Ovarian follicular development, lipid peroxidation, antioxidative status and immune response in laying hens fed fish oil-supplemented diets to produce n-3-enriched eggs

Including Walnuts in a Low-Fat/Modified-Fat Diet Improves HDL Cholesterol-to-Total Cholesterol Ratios in Patients With Type 2 Diabetes

Contact Info

Product

Resources

About