Oxidative stress and apoptosis in Asian honey bees (A. cerana) exposed to multiple pesticides in intensive agricultural landscape

Kumar, Debaditya; Banerjee, Debdas; Chakrabarti, Priyadarshini; Sarkar, Sagartirtha; Basu, Prasenjit

doi:10.1007/s13592-022-00929-2

Cited by 11 publications

(5 citation statements)

References 95 publications

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“…A diferencia de las otras sustancias analizadas, el Limoneno no tiene la molécula de oxígeno en su composición, que es capaz de desencadenar radicales libres dañinos para el cuerpo, lo que hace que el individuo sea susceptible al estrés oxidativo (Kumar et al, 2022). Además, ya hay un informe de que los monoterpenos pueden tener propiedades antioxidantes (Yu et al, 2017).…”

Section: Discussionunclassified

Inmunotoxicidad comparativa de abejas, Apis mellifera (Hymenoptera: Apidae), expuestas a xenobióticos naturales y sintéticos

Melo,

Teixeira,

Camara

et al. 2024

Revista Científica Multidisciplinar Núcleo do Conhecimento

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El objetivo de este estudio fue comparar los efectos de los pesticidas químicos naturales y sintéticos, examinando la supuesta selectividad de estos compuestos naturales sobre las abejas Apis mellifera. Los valores de LC50 utilizados en los bioensayos se obtuvieron de la investigación de Souza et al. (2023) y son los siguientes: Karate® (13,4 µL/100 mL), compuesto de limoneno (1.440 µL/100 mL) y Roundup® (712.290 µL/100 mL). Sin embargo, según informaron estos autores, se utilizó una concentración de 250 µL/100 mL para Azamax®. Estos valores de LC50, junto con una concentración de 250 µL/100 mL de Azamax®, se emplearon en los análisis inmunohistoquímicos utilizando el método TUNEL y PCNA en el intestino medio de las abejas. También se realizaron evaluaciones inmunológicas (óxido nítrico, fenoloxidasa y marcadores de estrés oxidativo TBARS y GSH) en abejas adultas obreras. Los tratamientos con xenobióticos no revelaron apoptosis o proliferación celular. Sin embargo, observamos degeneración epitelial, marcada por la presencia de células vacuoladas, lo que sugiere un proceso necrótico. Excepto por el compuesto de limoneno, todas las sustancias indujeron estrés oxidativo, lo que llevó a niveles elevados de TBARS. Aunque no hubo diferencias en los niveles de GSH, observamos alteraciones en el sistema inmunológico de estos insectos, caracterizadas por un aumento de la actividad de la fenoloxidasa y los niveles de NO2. Basándonos en los resultados obtenidos, es posible concluir que se debe tener precaución al utilizar pesticidas químicos en la agricultura, ya sean de origen sintético o natural, ya que tienen el potencial de causar daños histopatológicos e inmunológicos irreversibles. Este estudio también subraya la importancia de realizar investigaciones más exhaustivas sobre el impacto de los productos naturales en la fisiología de los insectos polinizadores.

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Section: Discussionunclassified

Inmunotoxicidad comparativa de abejas, Apis mellifera (Hymenoptera: Apidae), expuestas a xenobióticos naturales y sintéticos

Melo,

Teixeira,

Camara

et al. 2024

Revista Científica Multidisciplinar Núcleo do Conhecimento

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“…If the stress intensity exceeds a certain threshold or persists for an extended period, ER stress will ultimately result in cell apoptosis [35]. Additionally, XBP1s has been shown to enhance the synthesis of the CHOP transcription factor [36-38], thereby exacerbating honeybee cell apoptosis [39]. FBXO32 encodes a ubiquitin-E3 ligase that plays a role in regulating cellular autophagy and maintaining endoplasmic reticulum homeostasis [40].…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptomics uncovers apoptosis in brain impairment in newly pupated honeybee worker under cold stress

Zhu,

Cao,

et al. 2024

Preprint

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Background Honeybees have a much narrower developmental temperature range compared to other insects, making it an ideal model species for studying temperature effect on insect metamorphosis. Cold exposure during pupal stage resulted in impaired learning, memory, and foraging ability in adults. So far, the understanding of molecular mechanisms and physiological processes underlying the impaired brain development of honeybee pupae under cold exposure is unclear. Result The transcriptome data used in this study were collected from the heads of white-eyed honeybee pupae between control group and cold-treated groups. A total of 736 significantly differentially expressed genes (DEGs) were found to be shared by the different durations of cold treatment, and three gene clusters with significant expression patterns were identified using gene expression trend analysis, and RT-qPCR verification was performed on six genes. Enrichment analyses of each cluster using GO and KEGG database showed that upregulated DEGs followed by a plateau were significantly enriched in apoptosis, axon regeneration and signalling pathways regulating pluripotency of stem cells genes, consistently upregulated DEGs were significantly enriched in MAPK signalling pathway, and the downregulated DEGs followed by a plateau were related to insect ecdysteroids synthesis (phm and spookiest), epigenetic regulation of genes associated with brain development (LSD1), endoplasmic reticulum-associated protein degradation (RNF5 and SVIP) and regulation of endoplasmic reticulum stress-associated cell apoptosis (FBXO32, PET191 and UBL5). Conclusion Our study suggests that cold stress will inhibit the synthesis of ecdysteroids, disrupt gene epigenetic regulation, and intensify endoplasmic reticulum stress-associated brain cell apoptosis, thus hinder brain development in new pupal heads.

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“…Unlike the other analyzed substances, Limonene does not have the oxygen molecule in its composition, which is capable of triggering free radicals harmful to the body, making the individual susceptible to oxidative stress (Kumar et al, 2022). Furthermore, there is already a report that monoterpenes may have antioxidant properties (Yu et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Comparative immunotoxicity of bees, apis mellifera (hymenoptera: apidae), exposed to natural and synthetic xenobiotics

Melo,

Teixeira,

Camara

et al. 2024

Revista Científica Multidisciplinar Núcleo do Conhecimento

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The objective of this study was to compare the effects of natural and synthetic chemical pesticides, thereby examining the supposed selectivity of these natural compounds on Apis mellifera bees. The LC50 values used in the bioassays were obtained from the research by Souza et al. (2023) and are as follows: Karate® (13.4 µL/100 mL), Limonene compound (1,440 µL/100 mL), and Roundup® (712,290 µL/100 mL). However, as reported by these authors, a concentration of 250 µL/100 ml was used for Azamax®. These LC50 values, along with a concentration of 250 µL/100 mL of Azamax®, were employed in immunohistochemical analyzes using the TUNEL method and PCNA in the midgut of bees. Immunological assessments (nitric oxide, phenoloxidase, and oxidative stress markers TBARS and GSH) were also conducted on adult worker bees. The xenobiotic treatments did not reveal apoptosis or cell proliferation. Nonetheless, we observed epithelial degeneration, marked by the presence of vacuolated cells, suggesting a necrotic process. Except for the Limonene compound, all substances induced oxidative stress, leading to increased levels of TBARS. Although there were no differences in GSH levels, we observed alterations in the immune system of these insects, characterized by increased phenoloxidase activity and NO2 levels. Based on the acquired results, it is possible to conclude that caution should be exercised when using chemical pesticides in agriculture, whether they are of synthetic or natural origin, as they have the potential to cause irreversible histopathological and immunological damage. This study also underscores the importance of conducting more comprehensive investigations into the impact of natural products on the physiology of pollinator insects.

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Oxidative stress and apoptosis in Asian honey bees (A. cerana) exposed to multiple pesticides in intensive agricultural landscape

Cited by 11 publications

References 95 publications

Inmunotoxicidad comparativa de abejas, Apis mellifera (Hymenoptera: Apidae), expuestas a xenobióticos naturales y sintéticos

Inmunotoxicidad comparativa de abejas, Apis mellifera (Hymenoptera: Apidae), expuestas a xenobióticos naturales y sintéticos

Comparative transcriptomics uncovers apoptosis in brain impairment in newly pupated honeybee worker under cold stress

Comparative immunotoxicity of bees, apis mellifera (hymenoptera: apidae), exposed to natural and synthetic xenobiotics

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