Oxidative stress and apoptosis induced by hydroxyapatite nanoparticles in C6 cells

Xu, Jing; Xu, Pengjuan; Li, Zhigui; Huang, Jie; Yang, Zhuo

doi:10.1002/jbm.a.33270

Cited by 64 publications

(42 citation statements)

References 46 publications

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“…However, unlike the latter, HAp has the advantage of showing strong affinity for organic compounds and living organisms, bringing obvious advantages for instance if used for environmental remediation or cancer treatment. [35][36][37] Again, defects and dopants play a central role here. Electron spin resonance (ESR) studies clearly show that the surface reactive species is superoxide O •− 2 .…”

Section: Introductionmentioning

confidence: 99%

Sub-Band Gap Absorption Mechanisms Involving Oxygen Vacancies in Hydroxyapatite

et al. 2019

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Hydroxyapatite (HAp) is a widely used biomaterial for the preparation of bone and dental implants. Despite the relevance of HAp in medicine, exciting applications involving this material as a bio-compatible photocatalyst, depend on how well we understand its fundamental properties. Experimental evidence suggests that oxygen vacancies play a critical role in the production of surface radicals upon exposure of HAp to ultra-violet (UV) light. However, very little is known about the underlying physical and chemical details. We present a hybrid density-functional study of the structural and electronic properties of oxygen vacancies in large HAp supercells within the plane-wave formalism. We find that under equilibrium conditions, vacancies occur either as a simple vacant oxygen site (in the neutral charge state), or as extended structures replacing several crystalline moieties (in the double plus charge state). Large atomic relaxations upon ionization make the oxygen vacancy a negative-U defect, where the single plus charge state is metastable, being only accessible under UV excitation. From inspection of the transition levels, we find that electron promotion from the valence band top to the donor state of the vacancy, involves a zerophonon transition of 3.6-3.9 eV. This mechanism is the most likely explanation to the 3.4-4.0 eV absorption onset for the observation of photocatalysis using HAp under persistent UV illumination.

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Section: Introductionmentioning

confidence: 99%

Sub-Band Gap Absorption Mechanisms Involving Oxygen Vacancies in Hydroxyapatite

et al. 2019

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“…This helps in deceleration of atherosclerotic injury Lately recent research has unveiled several newer domains of metformin's action that produce potential beneficial effects in stressful conditions (Natarelli & Schober, 2015). Metformin improves mitochondrial homeostasis, thereby diminishes apoptosis induced by oxidative stress and prevents cell death (Xu, Xu, Li, Huang, & Yang, 2012). It boosts the antioxidant system by complementing mitochondrial function (Chandwaney, Leichtweis, Leeuwenburgh, & Ji, 1998;Gupta, Chellappan, Mishra, Malipeddi, & Dua, 2018).…”

Section: Introductionmentioning

confidence: 99%

Combinational effect of curcumin and metformin against gentamicin‐induced nephrotoxicity: Involvement of antioxidative, anti‐inflammatory and antiapoptotic pathway

et al. 2019

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Gentamicin (GM) is an antibiotic related to aminoglycoside group that is used in treating Gram‐negative bacterial infections. However, treatment with gentamicin is considered to be limited as it induces an oxidative stress‐mediated apoptosis in kidney which causes a nephrotoxicity. Metformin is a well‐known biguanide that is used for treating diabetes mellitus, especially type 2. Supplement with plant metabolites or natural antioxidants produce a protective activity against many types of diseases in vivo. Curcumin is a main medicinal constituent of Curcuma longa, has reported for number of biological effects, such as antioxidant, anti‐inflammatory, and antitumor. The study aims at evaluating the metformin and curcumin alone or in combination on nephrotoxicity induced by GM. The outcome of the study shows that both metformin and curcumin, when used unaided, were effectively decreasing GM‐induced nephrotoxicity. The two drugs combination was showed synergistic effect in ameliorating a GM‐induced kidney injury, as supported by expressively improved renal dysfunction. Metformin and curcumin showed strong protection against oxidative stress in GM treated animals through decreasing the activities and expression of various antioxidative enzymes. Moreover, combination of two drugs showed an anti‐inflammatory response through reducing a level of pro‐inflammatory cytokines including tumor necrosis factor‐alpha, interleukin 1‐beta, and interleukin 6 in GM intoxicated group of animals. Furthermore, GM agitated apoptosis was affectedly diminished by the combinational treatment of metformin and curcumin via down‐regulating activity of cleaved Caspase‐3 and pro‐apoptotic factor Bax, whereas increasing anti‐apoptotic factor Bcl‐2 signaling pathways. The above results suggested that combinational treatment of metformin and curcumin might be have a synergizing effect and substantial potential against nephrotoxicity induced by GM. Practical applications Curcumin and metformin combination exhibited substantial synergistic effect against GM‐induced nephrotoxicity through reducing oxidative stress, inflammation, as well as apoptosis in kidney cells. Therefore, the method of combination of curcumin and metformin might be functional to treat or inhibit GM prompted nephrotoxicity in future.

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“…Compared with viral vectors, which pose the danger of immunogenicity and pathogenicity, HAP has high osteoconductivity and favorable biocompatibility, and/or osteoinductivity without proinflammatory or immunogenic side effects (16)(17)(18). HAP inhibits tumor cell growth directly (19), and is a useful gene delivery system due to its efficiency, safety and economy (12).…”

Section: Inhibition Of Prostate Cancer Rm1 Cell Growth In Vitro By Hymentioning

confidence: 99%

Inhibition of prostate cancer RM1 cell growth in vitro by hydroxyapatite nanoparticle-delivered short hairpin RNAs against Stat3

Zhu

Wang

Guo

et al. 2017

Molecular Medicine Reports

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The present study investigated the effect of signal transducer and activator of transcription 3 (Stat3) interference on RM1 prostate cancer cell viability in vitro, using plasmid‑based Stat3 specific short hairpin RNA (sh‑Stat3) delivered by hydroxyapatite nanoparticles (HAP). HAP carrying sh‑Stat3 plasmids were transfected into tumor cells. MTT assays were used to measure RM1 cell viability 24 and 48 h following transfection, and the apoptosis rate and cell cycle phase distribution were determined by flow cytometry. Stat3 mRNA expression levels were measured by reverse transcription‑quantitative polymerase chain reaction and Stat3, Cyclin D1, B cell lymphoma 2 apoptosis regulator (Bcl‑2), vascular endothelial growth factor (VEGF), Bcl‑2 associated X apoptosis regulator (Bax) and cleaved‑caspase‑3 protein expression levels were detected using western blot analysis. The results demonstrated that HAP‑delivered sh‑Stat3 significantly decreased RM1 cell viability through the promotion of cell cycle arrest and apoptosis. Stat3 mRNA and protein expression levels were significantly downregulated in RM1 cells. Bcl‑2, VEGF and Cyclin D1 were also significantly downregulated, but cleaved‑caspase‑3 and Bax mRNA and protein expression levels were significantly upregulated. HAP‑delivered sh‑Stat3 decreased RM1 cell viability in vitro, and HAP assisted plasmid‑based delivery of shRNA into tumor cells. The present results suggest that HAP may be a useful method for successful shRNA delivery into tumors.

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Oxidative stress and apoptosis induced by hydroxyapatite nanoparticles in C6 cells

Cited by 64 publications

References 46 publications

Sub-Band Gap Absorption Mechanisms Involving Oxygen Vacancies in Hydroxyapatite

Sub-Band Gap Absorption Mechanisms Involving Oxygen Vacancies in Hydroxyapatite

Combinational effect of curcumin and metformin against gentamicin‐induced nephrotoxicity: Involvement of antioxidative, anti‐inflammatory and antiapoptotic pathway

Inhibition of prostate cancer RM1 cell growth in vitro by hydroxyapatite nanoparticle-delivered short hairpin RNAs against Stat3

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