Oxidative stress and band 3 protein function in Liza aurata and Salmo irideus erythrocytes: effect of different aquatic conditions

Abstract

The aim of this study was to assess the effect of the different aquatic conditions on anion transport of fish erythrocytes through the measurement of the sulphate uptake operating from band 3, the determination of reduced glutathione (GSH) and oxidized glutathione (GSSG). To this purpose, blood samples of 30 Liza aurata and 30 Salmo irideus fishes were collected via caudal vein and washed with physiological buffer. Successively, erythrocytes suspended at 3% haematocrit were used to measure the SO(4) (=) influx by atomic absorption spectrophotometry at 425 nm wavelength and the GSH concentration using an immunodiagnostic assay intended for the quantitative determination of glutathione in ethylenediaminetetraacetic acid in blood. All results were analyzed using unpaired Student's t-test and a P < 0.05 was considered statistically significant. The results of this study showed statistically significant differences about the sulphate uptake and the glutathione levels in S. irideus with respect to L. aurata (P < 0.0001). In conclusion, the different aquatic conditions play an important role on anion transport in fish erythrocytes, showing that environmental differences induce perturbations in erythrocyte membrane and should be evaluated to prevent physiological damages in fishes.

Effect of bisphenol A toxicity on growth performance, biochemical variables, and oxidative stress biomarkers of Nile tilapia,Oreochromis niloticus(L.)

“…Ozaydin et al () found that BPA significantly decreased the levels of GSH, SOD, GPx and CAT, and increased the levels of MDA in plasma of Wistar albino rats. In general, whenever Nile tilapia exposed to water pollution, it exerts metabolic mechanisms that help them for adaptation (Casella et al, ; Faggio et al, ; Fazio et al, ; Stephensen et al, ), detoxification (Pagano et al, ; Sheehan, Meade, Foley, & others, ;), and/or oxidative defense protection (Abdel‐Tawwab & Wafeek, ; Abdel‐Tawwab, El‐Sayed, Monier, et al, ; Geret & Bebianno, ).…”

Interactive effects of coffee bean supplementation and waterborne zinc toxicity on growth performance, biochemical variables, antioxidant activity and zinc bioaccumulation in whole body of common carp, Cyprinus carpio L.

“…Fish are able to live in different aquatic ecosystems including metal‐contaminated environments and they exert metabolic mechanisms that help them for adaptation (Casella et al., ; Faggio, Fedele, Arfuso, Panzera, & Fazio, ; Fazio et al., ; Stephensen et al., ), for detoxification (Pagano et al., ; Sheehan, Meade, Foley, & Dowd, ) and for the antioxidant protection (Geret & Bebianno, ). SOD is an important defence enzyme that catalyses the dismutation of O 2 , which produces H 2 O 2 that is converted to H 2 O and molecular oxygen by CAT (Qu et al., ).…”

Sulfate influx on band 3 protein of equine erythrocyte membrane (Equus caballus) using different experimental temperatures and buffer solutions

“…Hence, because plasma membrane anion exchange is a nearly ubiquitous function in vertebrate cells, it has been studied in greatest detail in the human erythrocyte . However, several reviewers have also focused on band 3 in fish erythrocyte membranes and on the effects of different aquatic conditions on anion transport of fish erythrocytes through the measurement of the sulfate uptake operating from band 3 . Other authors also studied the erythrocyte anion transport showing the difference between nucleated and anucleated red blood cells (RBCs) .…”