Oxidative stress and breast cancer biomarkers: the case of the cytochrome P450 2E1

Singh, Subir; Rajendran, Ramkumar; Kuroda, Kensuke; Isogai, Emiko; Krstic‐Demonacos, Marija; Demonacos, Constantinos

doi:10.20517/2394-4722.2016.42

Cited by 12 publications

(6 citation statements)

References 57 publications

(78 reference statements)

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“…1 mM H2O2, the MCF7 cells showed a 4-fold increase in SOH signal whereas for the MDA-MB-231 we recorded a 1.5-fold increase, relative to their starting basal levels. These data support previous findings showing variable basal levels of SOHs in different cells [38][39][40], which may be related to differences in the redox state of each specific cell line [41][42][43]. Noteworthy, we observed a distinct response between the two cell lines to the oxidative stress caused by treatment with H2O2, with the MCF7 being 2.6 times more susceptible than MDA-MB-231 in increasing the amount of SOHs following the addition of H2O2.…”

Section: Cellular Localization and Levels Of Protein Sulfenylation Insupporting

confidence: 90%

Small-molecule optical probes for cell imaging of protein sulfenylation and their application to monitor cisplatin induced protein oxidation

Cilibrizzi

Terenghi

Fedorova

et al. 2017

Sensors and Actuators B: Chemical

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Reactive oxygen species (ROS) are considered versatile second messengers mediating fundamental biological functions. A molecular pathway by which ROS determine functional diversity is the selective oxidation of cysteine residues to form sulfenic acid (SOH) products, known as sulfenylation or S-hydroxylation. This crucial post-translational modification is responsible for the alteration of protein stability, function and signalling. Despite considerable advances on the identification of sulfenic residues on individual proteins, improved methods are needed for direct visualization and accurate quantification of the extent of total protein sulfenylation. Herein we present the synthesis of two new cell-permeable fluorescent probes containing dimedone (a cyclic β-diketone with high specificity for sulfenic acids), and apply them to study oxidation processes in individual cells via microscopy. The low cytotoxicity, cell permeability and optical features of the probes allowed us to visualize and quantify the oxidation of cysteine residues in live cells during H2O2-mediated oxidative burst (i.e. exogenously administered H2O2). We present preliminary cellular imaging studies with these probes to analyse the oxidation process in cells treated with the anticancer drug cisplatin

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Section: Cellular Localization and Levels Of Protein Sulfenylation Insupporting

confidence: 90%

Small-molecule optical probes for cell imaging of protein sulfenylation and their application to monitor cisplatin induced protein oxidation

Cilibrizzi

Terenghi

Fedorova

et al. 2017

Sensors and Actuators B: Chemical

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“…In addition, antioxidant biomarker FRAP was associated with higher diet quality index score at baseline. Previous studies had investigated FRAP in women with breast cancer [ 42 , 43 , 44 ]. Evidence has shown that FRAP values were proportional to the reducing power of the main nonenzymatic antioxidants in plasma [ 27 ]; thus, this assay was selected to assess the antioxidant status of women with breast cancer in the present study.…”

Section: Discussionmentioning

confidence: 99%

“…Sateesh et al [ 42 ] found values of FRAP in women with breast cancer lower than the ones recorded for healthy women; this finding indicates that the disease leads to decreased nonenzymatic antioxidant defenses. Singh et al [ 44 ] found lower FRAP values after neoadjuvant chemotherapy application in women with breast cancer, which evidenced an even greater reduction in nonenzymatic antioxidant defense caused by the treatment. According to the present investigation, it seems that patients’ diet can enhance the nonenzymatic antioxidant defenses reduced by the disease, and it likely has a protective effect against oxidative damage in the case of lack of treatment.…”

Section: Discussionmentioning

confidence: 99%

Diet Quality Is Associated with Serum Antioxidant Capacity in Women with Breast Cancer: A Cross Sectional Study

et al. 2020

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Oxidative stress produced by adjuvant treatments is associated with cell injury; however, a healthy diet can help mitigate it. The aim of this study is to investigate the association between diet quality and oxidative stress parameters in women subjected to adjuvant treatment for breast cancer. The sample comprised 70 women. Oxidative stress biomarkers and diet quality parameters based on the Brazilian Healthy Eating Index—Revised (BHEI-R)—were evaluated at baseline (p0) and after adjuvant treatment (p1). Ferric reducing antioxidant potential (FRAP) was associated with diet quality at p0. BHEI-R scores were not different between p0 and p1; however, scores from total vegetables, total fruits, milk and dairy products, and meat, eggs and legumes were lower during treatment. On the other hand, lower sodium and saturated fat intake observed at p1 counterbalanced the BHEI-R score. Oxidative stress parameters have increased at p1, but they were not associated with diet quality; thus, changes in component intake were not enough to promote changes in oxidative stress during treatment. It appears that diet can enhance patients’ antioxidant defense before treatment, which could lead to better outcomes in the long term. Further investigations may help to clarify the association between diet and oxidative stress in women with breast cancer.

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“…Hormonal factors are relevant in the development of breast tumors. These factors are strongly related to oxidative stress (OS) through the generation of reactive oxygen species (ROS) as Subir Singh, Ramkumar Rajendran, et al [38] demonstrated in their study. They did a comparative study on two groups, namely one group with positive hormonal breast cancer receptors and another with negative hormonal receptors.…”

Section: Figure 5 Fras For the Studied Group Accordingmentioning

confidence: 99%

Production of Reactive Oxygen Species, Unbalanced by Endogenous Antioxidant Capture Mechanisms Monitored in Surgically Treated Breast Cancer

Rusu-Moldovan¹,

Gruia²,

Mirza³

et al. 2020

Rev. Chim.

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Oxidative stress is defined as an imbalance between the production of oxygen reactive species (ROS) and their endogenous capacity of capturing them. Oxidative stress activates multiple effects generated by the non-specific growth of the ROS and by the decrease of antioxidant concentrations and neutralization mechanisms. The most accurate understanding of the mechanisms underlying oxidative stress and the deepening of its implications in different cancers has become very important in recent decades. Cancer cells are usually subjected to high levels of ROS, which continually activates the malignant phenotype by stimulating cell proliferation � by increasing the mitotic rate, angiogenesis, invasiveness and metastasis rates. The role of the ROS in the etiology and progression of breast cancer is in continuous processing. However, less attention has been paid to the development of redox-targeted breast cancer therapy strategies. Excess ROS production is detrimental for the survival and proliferation of neoplastic cells, through the oxidative-destructive mechanisms of biomolecules essential for life. This study departs from the hypothesis that the tumor is an inductive factor of oxidative stress, and an antioxidant treatment prior to surgery may influence the response to surgical treatment in breast cancer patients. This response is highlighted by determining certain essential compounds of the redox balance, after initiating the antioxidant therapy. Moreover, we have tried to assess the possible biochemical changes given by age, tumor size and asymmetry/laterality of the neoplasm in the investigated patients, and to determine the prognostic or predictive factors of these changes.

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Oxidative stress and breast cancer biomarkers: the case of the cytochrome P450 2E1

Cited by 12 publications

References 57 publications

Small-molecule optical probes for cell imaging of protein sulfenylation and their application to monitor cisplatin induced protein oxidation

Small-molecule optical probes for cell imaging of protein sulfenylation and their application to monitor cisplatin induced protein oxidation

Diet Quality Is Associated with Serum Antioxidant Capacity in Women with Breast Cancer: A Cross Sectional Study

Production of Reactive Oxygen Species, Unbalanced by Endogenous Antioxidant Capture Mechanisms Monitored in Surgically Treated Breast Cancer

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