Oxidative stress and DNA damage induced by gamma irradiation in Korean lawngrass (Zoysia japonica Steud.)

Lee, Hyo-Jeong; Kim, Ye-Sol; YeongDeuk, Jo; BoKeun, Ha; Kim, Dong-Sub; Kim, Jin-Baek; Kang, Si-Yong; Kim, Sang-Hoon

doi:10.17660/ejhs.2016/81.6.3

Cited by 5 publications

(5 citation statements)

References 33 publications

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“…These results suggest that high dose rates induce stronger damage compared with low dose rates of chronic irradiation. It is generally acknowledged that higher irradiation doses lead to more severe biological damage [10,16,18]; thus, the results of the current study are consistent with those of previous studies.…”

Section: Discussionsupporting

confidence: 92%

“…The alkaline comet assay was performed as described previously [18]. Briefly, the first layer of base slides was prepared with 1% normal melting agarose.…”

Section: Comet Assaymentioning

confidence: 99%

“…Absorbance values were measured at 570 nm using an Infinite 200 Pro microplate reader (Tecan, Mannendorf, Switzerland) and converted into concentrations based on standard curve data. The level of lipid peroxidation was determined by measuring the MDA content using the thiobarbituric acid test, as described previously [18]. Absorbance values were measured at 450, 532, and 600 nm and converted to concentrations (nM g −1 fresh weight) using the following equation:…”

Section: H2o2 and Malonedialdehyde (Mda) Contentmentioning

confidence: 99%

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Effects of Acute and Chronic Gamma Irradiation on the Cell Biology and Physiology of Rice Plants

Choi

Han

et al. 2021

Plants

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The response to gamma irradiation varies among plant species and is affected by the total irradiation dose and dose rate. In this study, we examined the immediate and ensuing responses to acute and chronic gamma irradiation in rice (Oryza sativa L.). Rice plants at the tillering stage were exposed to gamma rays for 8 h (acute irradiation) or 10 days (chronic irradiation), with a total irradiation dose of 100, 200, or 300 Gy. Plants exposed to gamma irradiation were then analyzed for DNA damage, oxidative stress indicators including free radical content and lipid peroxidation, radical scavenging, and antioxidant activity. The results showed that all stress indices increased immediately after exposure to both acute and chronic irradiation in a dose-dependent manner, and acute irradiation had a greater effect on plants than chronic irradiation. The photosynthetic efficiency and growth of plants measured at 10, 20, and 30 days post-irradiation decreased in irradiated plants, i.e., these two parameters were more severely affected by acute irradiation than by chronic irradiation. In contrast, acutely irradiated plants produced seeds with dramatically decreased fertility rate, and chronically irradiated plants failed to produce fertile seeds, i.e., reproduction was more severely affected by chronic irradiation than by acute irradiation. Overall, our findings suggest that acute gamma irradiation causes instantaneous and greater damage to plant physiology, whereas chronic gamma irradiation causes long-term damage, leading to reproductive failure.

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Section: Discussionsupporting

confidence: 92%

“…The alkaline comet assay was performed as described previously [18]. Briefly, the first layer of base slides was prepared with 1% normal melting agarose.…”

Section: Comet Assaymentioning

confidence: 99%

Section: H2o2 and Malonedialdehyde (Mda) Contentmentioning

confidence: 99%

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Effects of Acute and Chronic Gamma Irradiation on the Cell Biology and Physiology of Rice Plants

Choi

Han

et al. 2021

Plants

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“…Figure 3 displayed that all control plants and wild type are negative toward this marker used together with 2J3 and 15L1 mutant lines, another 18 mutant lines shown two alleles from those of mutant lines detected, 85% of mutant lines were polymorphic to the marker used. Gamma rays is a physical mutagenic, producing free radicals from water radiolysis, free radicals is an unstable spur which would attack the chemical bonds randomly including chromosome and DNA of plants (Lee et al, 2016;Tanaka et al, 2010). The existence of genetic differences between the parents with their mutant can be assumed that the plants have been mutated.…”

Section: Resultsmentioning

confidence: 99%

Drought Tolerance and Evaluation of Genetic Changes in Rice Mutant Lines

Amsal¹,

Ishak-ishak²

2018

American Journal of Applied Sciences

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The genetic improvement of rice through mutation induction to obtain drought resistance is essential for stable and adequate rice production would enable us to anticipate the rice yield-reducing effects of global warming. The aim of this research was to evaluate the rice mutant lines showing tolerance to drought condition and their genetic relationship using SSR markers. Mutant lines were obtained by irradiation of Mira-1 rice variety at primordial state by 25 and 50 Gy doses. Twenty numbers of M4 rice mutant lines, cv. Mira-1 as wild type, cv. Gajah mungkur as positive control and cv. IR64 as negative control were chosen in this study were planted in PVC cylinders in the green house. The result showed that the agronomic trait of rice mutant lines were better than their wild type. Root length of 17 number of mutant lines are longer than their wild type and Gajah mungkur, the longest root obtain was 71.5 cm on 7J4 rice mutant line compared to Mira-1 only which was 25 cm. The number of lateral roots and seeds content per panicle higher than their wild type and positive control. Based on SSR marker linked to root depth, DRO1 showed that 12 numbers of mutant lines polymorphic and changing of genetic of mutant lines which was indicating the mutant lines were tolerant to drought compared to their wild type. The molecular information of rice mutant lines is essential for further development of a new superior variety of rice, which would be an improvement of the variety already existed.

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“…Therefore, it can penetrate deeply into the plant cells and possibly creates changes both at the levels of gene and chromosome. Based on research on several plants such as Triticum aestivum L. (Borzouei et al, 2010), rice (Haris et al, 2013), soybeans (Hanafiah et al, 2010), Korean lawngrass (Lee et al, 2016), Ciherang rice (Sulistyo et al, 2016), Zea mays L. (Rafiuddin et al, 2013), Glicine max L. (Mudibu et al, 2012), and sorghum (Surya & Soeranto, 2009) showed that gamma radiation at doses of 100 to 300 Gy had an effect on the genetic variety. Genetic veriety of mutant lines can be measured using morphological and molecular characterizations.…”

Section: Introductionmentioning

confidence: 99%

Growth and Production of Brachiaria brizantha cv. MG5 in Three Difference Regrowth Phase Treated by Gamma Radiation Dose

Respati

Umami

Hanim

2018

Trop. Anim. Sci. J.

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This study was aimed to determine the effect of dose of gamma radiation of seeds on plant growth and plant production of Brachiaria brizantha cv. MG5 during 3 different growth phases. The variables measured were plant growth parameters (height and length of plant, number of leaves and tillers) and production (dry matter and organic matter productions). Data were analyzed in a 5 x 3 factorial design. The first factor was dose of gamma radiation consisted of 5 levels i.e., 0, 100, 200, 300, and 400 Gy. The second factor was regrowth phase consisted of 3 levels i.e., growth phase 1, growth phase 2, and growth phase 3. The difference between means was analyzed using Duncan's Multiple Range Test (DMRT). The results showed that dose of gamma radiation influenced the plant growth and production (P<0.05) on different growth phases. The dose of 100 Gy gamma radiation showed the highest height and length, as well as dry matter and organic matter productions. The dose of 200 Gy gamma radiation showed the highest number of leaves and tillers. During regrowth phase 2, the grasses showed the highest of height, number of leaves and tillers, and organic matter production, while during regrowth phase 3, the grasses showed the highest length (P<0.05). There was an interaction between the dose of gamma radiation of seeds and regrowth phases on the growth and production of B. brizantha cv. MG5 (P<0.05). The dose of 100 Gy radiation of seeds and regrowth phase 2 gave the best interaction. In conclusion, the dose of 100 Gy radiation of seeds gave the best growth and production of B. brizantha cv. MG5 during regrowth phase 2.

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Oxidative stress and DNA damage induced by gamma irradiation in Korean lawngrass (Zoysia japonica Steud.)

Cited by 5 publications

References 33 publications

Effects of Acute and Chronic Gamma Irradiation on the Cell Biology and Physiology of Rice Plants

Effects of Acute and Chronic Gamma Irradiation on the Cell Biology and Physiology of Rice Plants

Drought Tolerance and Evaluation of Genetic Changes in Rice Mutant Lines

Growth and Production of Brachiaria brizantha cv. MG5 in Three Difference Regrowth Phase Treated by Gamma Radiation Dose

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