Oxidative stress and DNA damage induced by cadmium in the human keratinocyte HaCaT cell line: Role of glutathione in the resistance to cadmium

Nzengue, Yves; Steiman, R.; Garrel, Catherine; Lefebvre, Emmanuel; Guiraud, Pascal

doi:10.1016/j.tox.2007.10.005

Cited by 105 publications

(53 citation statements)

References 78 publications

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“…Experimental evidence for induced synthesis of glutathione by oxidative stress has been reported by Nzengue and coworkers (32). They found that when cultured human cells were treated with cadmium, the GSSG level increased and the ratio of the GSH to GSSG level decreased with concomitant increases in oxidative damage to proteins, lipids and DNA.…”

Section: Discussionmentioning

confidence: 79%

Exposure to Cigarette Smoke Increases Urate Level and Decreases Glutathione Level in Larval Drosophila melanogaster

Fujiwara

Hamatake

Arimoto

et al. 2011

Genes and Environment

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Recently, we reported experimental evidence to support the notion that in Drosophila melanogaster, urate is involved in defense against toxic eŠects of environmental cigarette smoke (ECS). To obtain further information pertaining to the defense mechanisms involving urate and other antioxidants, the present study measured the levels of urate, its precursors and glutathione, and SOD activity in larval ‰ies of wild-type strains (Oregon-R and Canton-S) and two urate-null mutant strains (ma-l and ry 1 ) following exposure to ECS for various durations. In both wild type strains, unlike the case in either of the mutant strains, the urate level signiˆcantly increased above the basal level in a manner dependent on the duration of ECS exposure. Similar increases in the level of urate precursors were found in Canton-S and in both of the urate-null strains. There was a slight increase in glutathione level above the control level following ECS exposure for a short time, followed by an exposure-dependent decrease to less than 60% of the control level within the exposure range used in all of the four strains. On the other hand, no appreciable change was found in the SOD activity prior to or following ECS exposure, irrespective of the strain examined. In terms of the survival of treated larvae to adulthood under the conditions used for the measurements of urate and others, it was found that wild-type strain Canton-S was as sensitive as the urate-null mutant strains and clearly more sensitive than wild-type strains Oregon-R and Hikone-R. This was so despite the fact that, compared with Oregon-R, Canton-S contained urate at relatively higher levels prior to and following ECS exposure, and that the glutathione levels in Canton-S prior to and following treatment were comparable with those in other strains. These results are discussed with respect to the involvement of urate and glutathione in defense against the toxicity of ECS and the possible existence of another defense mechanism which is deˆcient in the Canton-S strain.

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Section: Discussionmentioning

confidence: 79%

Exposure to Cigarette Smoke Increases Urate Level and Decreases Glutathione Level in Larval Drosophila melanogaster

Fujiwara

Hamatake

Arimoto

et al. 2011

Genes and Environment

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“…One unit of SOD activity is defined as the amount of the enzyme required to inhibit the rate of pyrogallol auto-oxidation by 50%. CAT activity was determined as described previously (Nzengue et al 2008). Activity was assayed by determining the rate of decomposition of H 2 O 2 by CAT in 10 mM potassium phosphate buffer (pH 7).…”

Section: Antioxidant Enzyme Activity Assaysmentioning

confidence: 99%

Developmental changes in antioxidant enzymatic defences against oxidative stress in sheep placentomes

Garrel¹,

Fowler²,

Al-Gubory³

2010

Journal of Endocrinology

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Early pregnancy is susceptible to oxidative stress, and thus characterisation of antioxidant systems and pro-and antiapoptotic pathways would improve understanding of placental development and function. We aimed, therefore, to determine the activities of the antioxidant enzymes, copper/zinc-superoxide dismutase (SOD1), manganese-SOD (SOD2), catalase (CAT), glutathione (GSH) peroxidase (GPX) and GSH reductase (GSR); and to quantify the expression of BAX and MCL1 proteins in relation to the developmental changes in antioxidant defences in sheep placentomes sampled on days 35, 55 and 80 of pregnancy. Placentome progesterone content was analyzed to determine steroidogenic capacity. Malondialdehyde (MDA) and protein carbonyl were quantified in placentomes as biomarkers of lipid peroxidation and protein damage respectively. Placentome tissues demonstrated significantly increased content of progesterone and MDA at day 80 of pregnancy and protein carbonyl as early as day 50 of pregnancy. Progesterone and MDA contents were not different between days 35 and 55 of pregnancy. While SOD1 and CAT activities did not alter significantly, SOD2 activity decreased from days 35 to 55. GPX activity increased from days 35 to 55 and increased further to day 80 of pregnancy. GSR activity increased from days 35 to 55 of pregnancy. BAX protein expression decreased, while MCL1 increased from days 35 to 55 and 80 of pregnancy. The increased GPX activity was associated with a decrease in the BAX/MCL1 protein expression ratio. Changes in the antioxidant enzymatic defences could be a part of placentome adaptation to reactive oxygen speciesinduced oxidative stress at specific early developmental stages of pregnancy.

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“…A deficiency in GSH may account for some of the observed increase in ROS in Cd-treated cells (Liu et al, 2009). It is known that GSH depletion can lead to increased Cd sensitivity (Nzengue et al, 2008), and that artificial elevation of intracellular GSH levels can protect against Cd toxicity (Aiba et al, 2008). However chronic exposure to Cd can lead to an elevation in intracellular GSH levels (Kamiyama et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Differential sensitivity and responsiveness of three human cell lines HepG2, 1321N1 and HEK 293 to cadmium

Lawal

Ellis

2010

J. Toxicol. Sci.

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-Cadmium (Cd) is a toxic heavy metal with no uniform mechanism of toxicity. In this the present study, the toxic effect of 5, 10 and 50 μM of Cd chloride was compared in three human cell lines; a human hepatoma cell line HepG2, a human astrocytoma cell line 1321N1, and a human embryonic kidney cell HEK 293. The results indicate a decrease in the viability of all three cell lines following exposure to Cd with HepG2 cells (IC 50 = 13.96 μM) showing the most sensitivity when measured using the MTT assay. There was significant increase in lactate dehydrogenase leakage, DNA damage, malondialdeyde and antioxidant enzymes activities in all three cell lines especially at 50 μM Cd. Significant decreases in ATP production were also observed at all Cd concentrations in HepG2 and HEK 293 cell lines. ROS levels significantly increase and GSH/GSSG ratio significantly decrease in all the three cell lines after Cd exposure, but these effects were attenuated by the presence of N-acetylcysteine (NAC). The present study therefore shows that ROS production and glutathione (GSH) depletion may play a role in Cd-induced toxicity in all the three cell lines. The endogenous levels of protective enzymes as well as their responsiveness are likely to determine a cell's susceptibility to Cd.

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Oxidative stress and DNA damage induced by cadmium in the human keratinocyte HaCaT cell line: Role of glutathione in the resistance to cadmium

Cited by 105 publications

References 78 publications

Exposure to Cigarette Smoke Increases Urate Level and Decreases Glutathione Level in Larval Drosophila melanogaster

Exposure to Cigarette Smoke Increases Urate Level and Decreases Glutathione Level in Larval Drosophila melanogaster

Developmental changes in antioxidant enzymatic defences against oxidative stress in sheep placentomes

Differential sensitivity and responsiveness of three human cell lines HepG2, 1321N1 and HEK 293 to cadmium

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