Oxidative stress and enhanced paracellular permeability in the small intestine of methotrexate-treated rats

Maeda, Tomoko; Miyazono, Yuko; Ito, Kousei; Hamada, Kazuma; Sekine, Satoshi; Horie, Toshiharu

doi:10.1007/s00280-009-1119-1

Cited by 61 publications

(36 citation statements)

References 30 publications

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“…However, many factors such as pathogens, polyunsaturated fatty acids, and mildewed or oxidized feed can induce the oxidative stress (Slater, 1984;Halliwell and Chirico, 1993;Moreto and Perez-Bosque, 2009), especially in neonatal piglets (Chamulitrat et al, 1996;Aw, 1999;Lackeyram et al, 2012). These "stressors" induce intestinal injury and dysfunction by producing excessive reactive oxygen species (ROS) and destroy the antioxidant capacity in neonatal piglets (Moreto and Perez-Bosque, 2009;Maeda et al, 2010). Also, a series of pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α) and interleukins-1α, -1β, -6 (IL-1α, IL-1β, IL-6) also are produced (Kelley, 2001).…”

Section: Introductionmentioning

confidence: 99%

Effects of soybean isoflavone on intestinal antioxidant capacity and cytokines in young piglets fed oxidized fish oil

Huang

Jiang

et al. 2016

J. Zhejiang Univ. Sci. B

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Abstract:To investigate the effect of glycitein, a synthetic soybean isoflavone (ISF), on the intestinal antioxidant capacity, morphology, and cytokine content in young piglets fed oxidized fish oil, 72 4-d-old male piglets were assigned to three treatments. The control group was fed a basal diet containing fresh fish oil, and the other two groups received the same diet except for the substitution with the same dosage of oxidized fish oil alone or with ISF (oxidized fish oil plus ISF). After 21 d of feeding, supplementation of oxidized fish oil increased the levels of malondialdehyde (MDA), oxidized glutathione (GSSG), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), interleukin-2 (IL-2), nuclear factor κ B (NF-κB), inducible nitric oxide synthase (iNOS), NO, and Caspase-3 in jejunal mucosa, and decreased the villous height in duodenum and the levels of secretory immunoglobulin A (sIgA) and IL-4 in the jejunal mucosa compared with supplementation with fresh oil. The addition of oxidized fish oil plus ISF partially alleviated this negative effect. The addition of oxidized fish oil plus ISF increased the villous height and levels of sIgA and IL-4 in jejunal mucosa, but decreased the levels of IL-1β and IL-2 in jejunal mucosa (P<0.05) compared with oxidized fish oil. Collectively, these results show that dietary supplementation of ISF could partly alleviate the negative effect of oxidized fish oil by improving the intestinal morphology as well as the antioxidant capacity and immune function in young piglets.

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Section: Introductionmentioning

confidence: 99%

Effects of soybean isoflavone on intestinal antioxidant capacity and cytokines in young piglets fed oxidized fish oil

Huang

Jiang

et al. 2016

J. Zhejiang Univ. Sci. B

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“…In order to minimize side-effects of MTX in patients, the studies focused on antioxidants. [28][29][30][31] One of them, GE, was mostly emphasized on its antioxidant properties in a number of in vitro and in vivo studies. 17,32,33 Various studies also presented that GE has been tried in the repair of MTX-induced intestinal injury.…”

Section: Discussionmentioning

confidence: 99%

The Effectiveness of Grape Extract in Rats with Methotrexate Induced Intestinal Mucositis

Yılmaz¹,

Dağlıoğlu²,

Bayram³

et al. 2012

Turkiye Klinikleri J Med Sci

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A AB BS ST TR RA AC CT T O Ob bj je ec ct ti iv ve e: : To investigate histopathologically whether grape extract (Vitis Vinifera L), a potent free radical scavenger and can ameliorate the methotrexate -induced intestinal mucositis. M Ma at te er ri ia al l a an nd d M Me et th ho od ds s: : Wistar albino male rats were assigned to 3 groups: Group 1 (n=7) control group: An equal volume of distilled water that used for grape extract was given throughout the 15 days and physiological saline was administered intraperitoneally on the 10th day. Group 2 (n=10) methotrexate group: Rats received an equal volume of distilled water. A single dose of 20 mg/kg methotrexate was given intraperitoneally on the 10th day. Group 3 (n=10) methotrexate-grape extract group: The same dose of methotrexate was applied intraperitoneally on the 10 th day. For milliliters grape extract solution was administered for 15 consecutive days. Complete blood counts were analyzed from each rat. R Re es su ul lt ts s: : The control group showed normal intestinal structure. Group 2 had villus shortening and fusion with variable degrees of epithelial atrophy (p=0.001). The number of crypt cells was decreased and crypt loss was prominent (p=0.007). Inflammatory infiltration and decreased goblet cell number in both villi and crypts were distinctive (p<0.001). Villous and crypt injury, and inflammatory infiltration was less in group 3. In addition, goblet cells were increased. The difference was significant between group 2 and 3 for the jejunum damage (p=0.021). Hemoglobin levels increased in group 3. C Co on nc cl lu us si io on n: : Present study suggested that grape extract could be useful in the ameliorating intestinal damage caused by methotrexate. Its effect could be attributed to its antioxidant properties. Grape extract may have a clinical application in cancer chemotherapy.K Ke ey y W Wo or rd ds s: : Methotrexate; mucositis; grape seed extract; intestinal diseases Ö ÖZ ZE ET T A Am ma aç ç: : Potansiyel bir serbest radikal yok edici olan üzüm ekstresinin (Vitis Vinifera L) metotreksat ile oluşturulmuş ince bağırsak mukozitini histopatolojik olarak iyileştirip iyileştirmediğini araştırmak. G Ge er re eç ç v ve e Y Yö ön nt te em ml le er r: : Wistar albino erkek sıçanlar 3 gruba ayrıldı. Grup 1 (n=7) kontrol grup: Üzüm ekstresi ile aynı miktarda olan distile su 15 gün boyunca kullanıldı ve 10. günde serum fizyolojik intraperitoneal olarak verildi. Grup 2 (n=10) metotreksat grubu: Sıçanlar aynı miktar distile su aldılar. Tek doz 20 mg/kg metotreksat 10. günde verildi. Grup 3 (n=10) Metotreksatüzüm ekstresi grubu: Aynı doz metotreksat 10. günde intraperitoneal olarak uygulandı. Dört mililitre üzüm ekstresi solüsyonu 15 gün boyunca alındı. Tam kan sayımı her bir sıçandan analiz edildi. B Bu ul lg gu ul la ar r: : Kontrol grup normal ince bağırsak yapısını gösterdi. Grup 2 değişik derecelerde epitel atrofisi ile birlikte villüs kısalması ve füzyonuna sahipti (p=0.001). Kript hücrelerinin sayısı azalmış ve kript kaybı belirgindi (p=0.007). Hem villüs...

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“…In this respect, the present study has shown treatment of the differentiated epithelial monolayer with the mercurial forms to result in an increased transport of LY, and thus in an increase in paracellular permeability. Some studies have shown oxidative and nitrosative stress of the intestinal mucosa to precede the increase in paracellular permeability (Maeda et al, 2010) and thus the loss of barrier function. Moreover, cytokine TNFα was found to be overexpressed in many treatments in our study, and has also been described as the cause of increases in intestinal permeability (Ma et al, 2004).…”

Section: Tablementioning

confidence: 99%

In vitro evaluation of inorganic mercury and methylmercury effects on the intestinal epithelium permeability

Vázquez

Vélez

Devesa

2014

Food and Chemical Toxicology

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Oxidative stress and enhanced paracellular permeability in the small intestine of methotrexate-treated rats

Abstract: NAC protected the small intestine of rats from MTX-induced change in paracellular permeability, suggesting that ROS played an important role in the enhanced paracellular permeability.

Cited by 61 publications

References 30 publications

Effects of soybean isoflavone on intestinal antioxidant capacity and cytokines in young piglets fed oxidized fish oil

Effects of soybean isoflavone on intestinal antioxidant capacity and cytokines in young piglets fed oxidized fish oil

The Effectiveness of Grape Extract in Rats with Methotrexate Induced Intestinal Mucositis

In vitro evaluation of inorganic mercury and methylmercury effects on the intestinal epithelium permeability

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