Oxidative stress and fish immune system: phagocytosis and leukocyte respiratory burst activity

Biller, Jaqueline Dalbello; Takahashi, Leonardo Susumu

doi:10.1590/0001-3765201820170730

Cited by 210 publications

(92 citation statements)

References 84 publications

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“…These results suggest that organic acids had no effect on the haematopoietic system. Leucocyte plays an important role in the cellular immunity and disease resistance of fish, so they are important indicators of non‐specific immune response (Biller & Takahashi, 2018). In the current study, a diet of 0.2% MA + 0.2% CA increased the total white blood cell count.…”

Section: Discussionmentioning

confidence: 99%

Effects of malic acid and citric acid on growth performance, antioxidant capacity, haematology and immune response of Carassius auratus gibelio

Zhang

Xia³

et al. 2020

Aquac Res

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The aim of this study was to investigate the effects of citric acid (CA) and malic acid (MA) on growth performance, antioxidant capacity, haematology and immune response of Carassius auratus gibelio. Carassius auratus gibelio were randomly divided into six groups, with three replicates in each group and 20 fish in each replicate.The six groups were fed a basic diet with organic acid supplementation (control group, 0.2%MA, 0.2%MA + 0.1%CA, 0.2%MA + 0.2%CA, 0.2%MA + 0.4%CA and 0.2%MA + 0.6%CA) for 8 weeks respectively. The results showed that compared with the control group, the weight gain rate and specific growth rate significantly increased in organic acid-fed groups (p < .05). The highest value was in the 0.2%MA + 0.2%CA group. However, there were no significant differences in survival rate, red blood cells or white blood cells between groups. The contents of ALT, AST and LDH in Carassius auratus gibelio fed with organic acids increased, especially in 0.2%MA + 0.4%CA and 0.2%MA + 0.6%CA groups. However, there was no significant difference between the 0.2%MA + 0.2%CA group and the control group. In addition, the highest values of GSH and T-AOC were found in the 0.2%MA + 0.6%CA group. Compared with the control group, there were no significant differences in the expression of HSP70 or LZM (p > .05), but the expression of Il-1β, IL-10 and TNF-α was up-regulated in the 0.2%MA + 0.4%CA and 0.2%MA + 0.6%CA groups. The results showed that the growth performance, antioxidant capacity, haematological parameters and expression of immune-related genes were significantly improved by adding organic acids to the feed of Carassius auratus gibelio. However, excessive addition of organic acids may cause liver injury, and the optimal addition amount in this experiment was 0.2%MA + 0.2%CA. K E Y W O R D S antioxidant capacity, citric acid, growth performance, haematological parameters, immune response, malic acid| 2767 ZHANG et Al. Ash 7.12% 7.12% 7.12% 7.12% 7.12% 7.12%

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Section: Discussionmentioning

confidence: 99%

Effects of malic acid and citric acid on growth performance, antioxidant capacity, haematology and immune response of Carassius auratus gibelio

Zhang

Xia³

et al. 2020

Aquac Res

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“…SOD, GPx and CAT are important antioxidant enzymes (Biller & Takahashi, 2018;Ighodaro & Akinloye, 2018). SOD catalyses superoxide radicals and produces O 2 and hydrogen peroxide (H 2 O 2 ).…”

Section: Discussionmentioning

confidence: 99%

Effect of dietary carob (Ceratonia siliqua) syrup on blood parameters, gene expression responses and ammonia resistance in tilapia (Oreochromis niloticus)

Yılmaz

2020

Aquac Res

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In this study, carob (Ceratonia siliqua) syrup was supplemented in the diet of tilapia (Oreochromis niloticus), and its effects on growth performance, immunological and serum biochemical parameters, gene expression responses and ammonia resistance of the fish were investigated. Carob syrup was added to the fish diet in ratios of 0%, 5%, 2.5%, 1.25% and 0.625%, such that five different diets were prepared: control, Cerat5000, Cerat2500, Cerat1250 and Cerat625 respectively. The fish were fed with experimental diets for 60 days. The survival rate of fish fed with 5% carob syrup decreased compared with all other experimental groups. Thus, this study found that feeding tilapia fish with 1.25% carob syrup for 60 days increased gene expression responses, some innate immune parameters and ammonia resistance of the fish without negative effects on growth performance or serum biochemical variables.

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“…Chemiluminescent activity of monocytes characterizes the state of their respiratory burst [3,30]. We used two chemiluminescent indicators: lucigenin and luminol.…”

Section: Discussionmentioning

confidence: 99%

Dependence of phenotype and chemiluminescent activity of monocytes on the Tregulatory cells content in patients with kidney cancer

et al. 2020

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The aim of this work was to reveal the interrelations between the number of T regulatory cells (Tregs) in patients with kidney cancer (KC) and phenotype of peripheral blood monocytes and their capacities to produce ROS. Patients with KC (T3N0M0, clear cell type) were examined prior to surgical treatment. Tregs phenotype and blood monocytes were identified by flow cytometry. ROS production of purified monocytes was carried out through the determination of lucigenin- and luminol-dependent spontaneous and zymosan-induced chemiluminescence activity. It has been found that the relative number of Tregs within total lymphocyte subset in KC patients was increased if compared to control values (in KC patients — Me = 6.3%). Then the patients were divided into two groups according to the median of Tregs number (less and more than 6.3%). The most pronounced changes in the phenotype of monocytes and their chemiluminescent activity were found in KC patients with the Tregs count of less than 6.3%. Our findings suggest that low frequency of Tregs in the periphery was associated with increased relative numbers of “intermediate” and “non-classical” (“pro-inflammatory”) monocytes as it was shown on the samples from patients with KC with a low level of Tregs. According to our data, both groups of KC patients had low levels of HLA-DR expression when comparing to control group. Furthermore, both groups of patients had decreased rates of HLA-DR and CD64 co-expressing cells. Changes in the phenotype of monocytes in patients with KC were closely linked with imbalance in ROS production. Thus, the monocytes spontaneous superoxide radical (primary ROS) synthesis in KC patients with a low Treg numbers were characterized by redused NADPH-oxidase activation time and increased level of its activity if compared to patients with a high Treg rates in peripheral blood. Next, the activation index for lucigenin-dependent chemiluminescence in KC patients was reduced, as well as it was independent of circulating Tregs rates and was determined apparently by the insufficiency of metabolic reserves. Similarly, spontaneous secondary ROS production by the monocytes in KC patients was lower then in healthy controls and was also independent of circulating Tregs rates. Finally, the induced secondary ROS synthesis and activation index for their synthesis in monocytes were reduced only in patients with KC with a low number of Tregs in the blood. In general, the characteristics of the chemiluminescent reaction of monocytes in patients with KC determined the imbalance in peripheral blood monocytes primary and secondary ROS production. Monocytes in patients with KC with a low number of Tregs in the blood were characterized by more pro-inflammatory activity due to the rapid activation and intensity of the synthesis of primary ROS.

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Oxidative stress and fish immune system: phagocytosis and leukocyte respiratory burst activity

Cited by 210 publications

References 84 publications

Effects of malic acid and citric acid on growth performance, antioxidant capacity, haematology and immune response of Carassius auratus gibelio

Effects of malic acid and citric acid on growth performance, antioxidant capacity, haematology and immune response of Carassius auratus gibelio

Effect of dietary carob (Ceratonia siliqua) syrup on blood parameters, gene expression responses and ammonia resistance in tilapia (Oreochromis niloticus)

Dependence of phenotype and chemiluminescent activity of monocytes on the Tregulatory cells content in patients with kidney cancer

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