Oxidative stress and genotoxicity biomarker responses in tilapia (Oreochromis niloticus) exposed to environmental concentration of 1-nitropyrene

Bacolod, Eugene T.; Uno, Seiichi; Villamor, Shiela S.; Koyama, Jiro

doi:10.1016/j.marpolbul.2017.01.077

Cited by 18 publications

(7 citation statements)

References 37 publications

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“…In vitro studies utilizing the rainbow trout liver cell line RTLW1, revealed that the exposure to the PAHs carbazole, acridine and dibenzothiophene, resulted in increased MN frequency, suggesting genotoxic impact (Brinkmann et al 2014). In Oreochromis niloticus exposed to nitrated PAHs, MN frequencies were signi cantly higher in the exposure groups after 7 and 14 days (Bacolod et al 2017). Similarly, two anuran species (Physalaemus cuvieri and Leptodactylus fuscus) exposed to 2mg/kg BaP had increased frequency of MN after 7 days (Fanali et al 2018).…”

Section: Discussionmentioning

confidence: 96%

Enzymatic, morphological, and genotoxic effects of benzo[a]pyrene in rainbow trout (Oncorhynchus mykiss)

Fanali

Oliveira

Sturve

2021

Environ Sci Pollut Res

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Fish have defense systems that are capable of repairing damages caused by xenobiotics like benzo[a]pyrene (BaP), so the aims of this study were to identify BaP toxicity in melanomacrophages (MMs) cytoskeleton, evaluate the melanin area in MMs and analyze genotoxicity. Rainbow trout juveniles (n = 24) were split in 48h and 7d treatments that received 2 mg/kg of BaP. After the experiment, blood samples were collected and liver was removed, to proceed with the analysis: EROD activity, MMs melanin area quanti cation, melanosomes movements, and a genotoxicity test. The results revealed increased in EROD activity after 48h and 7d BaP exposure. The group 7d displayed a reduction in MMs pigmented area, melanosomes aggregation, in addition to an increased frequency of micronuclei. By means of the EROD assay, it was possible to con rm the activation of BaP biotransformation system. The impairment of the melanosomes' movements possibly by an inactivation of the protein responsible for the pigment dispersion consequently affects the melanin area and thus might negatively impact the MMs detoxi cation capacity. In addition to this cytotoxicity, the increased frequency of micronucleus might also indicate the genotoxicity of BaP in this important sh species.

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Section: Discussionmentioning

confidence: 96%

Enzymatic, morphological, and genotoxic effects of benzo[a]pyrene in rainbow trout (Oncorhynchus mykiss)

Fanali

Oliveira

Sturve

2021

Environ Sci Pollut Res

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“…However, previous studies pointed out the adverse effects of water chemical pollutants particularly herbicides in different fish species (Nwani et al, 2011). Oreochromis niloticus was selected as an indicator for water pollution (Bacolod et al, 2017). Additionally, fish represent different adverse effects from thiobencarb herbicides (Bailey, 1993;Fernández-Vega et al, 1999;Saka, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…Genotoxic and mutagenic effects of herbicides have been previously evaluated in several model organisms (Sanderson et al, 2001;Hladik et al, 2008;Benfeito et al, 2014) using several biomarkers for genotoxicity assessment, such as DNA adducts, chromosomal aberrations, DNA strands breaks, micronuclei formation and cell death induction (Stein et al, 1993;Sikka et al, 1990;Bombail et al, 2001). Oreochromis niloticus fish have been used in several studies as a model for genotoxicity investigation of various environmental pollutants (Lima et al, 2006;Kandiel et al, 2014;Bacolod et al, 2017). Several studies have been conducted implementing relatively simple methods to verify the mutagenic effects of the herbicides.…”

Section: Introductionmentioning

confidence: 99%

S-Methyl Cysteine Protective Effects in Oreochromis Niloticus Fish Contaminated by Thiobencarb Herbicide

Elmadawy¹,

Abdo²,

Omar³

et al. 2020

WVJ

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Thiobencarb which is a carbamate herbicide is used for managing undesirable weeds during rice cultivation in Egypt. This study was designed to investigate the adverse effects of a field dose of thiobencarb on Nile tilapia and ameliorating the role of the low dose of S-methyl cysteine (SMC). Experimental fishes were divided into four groups; first group was reared without any treatments and served as a control group; the second group was exposed to thiobencarb (36µg/L); the third group was fed on a commercial feed containing 200 mg of SMC/Kg in conjunction with thiobencarb added to aquarium (36µg/L) while, the fourth group was fed on a feed containing 200 mg of SMC/Kg only. Fishes were sacrificed at the end of the experimental course (two months) and sampling was carried out. Catalase, Glutathione S Transferase activities, Glutathione reduced, and Malondialdhyde levels were assayed. Genotoxic effect of thiobencarb and SMC on treated fish was investigated in erythrocytes, gills, and liver tissues using micronucleus and comet assay. Histopathological examination of livers, gills, and brain was also carried out. The results indicated that fish exposed to thiobencarb indicated herbicide dependent oxidative stress and genotoxic effect justified by a significant difference in antioxidant biomarkers as well as nuclear abnormalities and comet parameters compared to control values. Moreover, histopathological findings were in line with other results. SMC ameliorated the adverse effects which were effective in the improvement of DNA and oxidative damage in thiobencarb intoxicated fish.

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“…In a setup by Bacolod, et. al., detection limit (LOD) of 1-NP was 2 ng/kg in fish and 0.002 ng/L in water with recoveries from 95 to 98% [11].…”

Section: Analysis Of 1-npmentioning

confidence: 99%

1-Nitropyrene Exposure as Genotoxicity and Oxidative Stress Biomarker

Abrea¹

2020

Preprint

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Oxidative stress and genotoxicity biomarker responses in tilapia (Oreochromis niloticus) exposed to environmental concentration of 1-nitropyrene

Cited by 18 publications

References 37 publications

Enzymatic, morphological, and genotoxic effects of benzo[a]pyrene in rainbow trout (Oncorhynchus mykiss)

Enzymatic, morphological, and genotoxic effects of benzo[a]pyrene in rainbow trout (Oncorhynchus mykiss)

S-Methyl Cysteine Protective Effects in Oreochromis Niloticus Fish Contaminated by Thiobencarb Herbicide

1-Nitropyrene Exposure as Genotoxicity and Oxidative Stress Biomarker

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