Oxidative stress and interleukin-6 secretion during the progression of type 1 diabetes

Abstract

Objective: To evaluate inflammatory, oxidizing, and reducing responses during the progression of type 1 diabetes mellitus (T1DM) in patients without chronic complications. Subjects and methods: Plasma antioxidant status, reactive oxygen species (ROS), and interleukin-6 (IL-6) were measured in 42 patients with T1DM and in 24 healthy subjects. Results: Significant increases were detected in the median values of ROS and IL-6 in patients with T1DM compared with healthy subjects (ROS ~ 4,836 vs. 2,036 RLU/min, respectively; P < .05: IL-6 ~ 14.2 vs. 9.7 pg/mL, respectively; P = .002). No significant between-group differences (P > 0.05) were observed in oxidizing responses or in IL-6 concentrations when diabetic patients were grouped according to time after diagnosis (0 -10, 10 -20 and > 20 years). Plasma antioxidant responses were similar in patients with T1DM and in healthy subjects. Conclusions: Our results demonstrate that oxidizing and inflammatory responses are increased at the onset of T1DM, but remain unchanged during disease progression. These findings suggest that functional changes involved in diabetic complications may commence in the first years after diagnosis. Arq Bras Endocrinol Metab.2012;56(7):441-8 Keywords Type 1 diabetes; disease progression; oxidative stress; plasma antioxidant status RESUMO Objetivo: Avaliar as respostas inflamatória, oxidativa e redutora na progressão do diabetes melito tipo 1 (DM1) em pacientes sem complicações crônicas. Sujeitos e métodos: Capacidade antioxidante do plasma, espécies reativas de oxigênio (ROS) e interleucina-6 (IL-6) foram avaliadas em 42 pacientes com DM1 e 24 indivíduos saudáveis. Resultados: Aumentos significativos foram detectados nas medianas de ROS e IL-6 em pacientes com DM1 comparados com indiví-duos saudáveis (ROS ~ 4.836 vs. 2.036 RLU/min, respectivamente, P < 0,05: IL-6 ~ 14,2 vs. 9,7 pg/ mL, respectivamente, P = 0,002). Diferenças não significativas (P > 0,05) foram observadas na resposta oxidante e IL-6 quando os diabéticos foram agrupados de acordo com o tempo após o diagnóstico (0-10, 10-20 e > 20 anos). A resposta antioxidante do plasma foi semelhante em pacientes com DM1 e em indivíduos saudáveis. Conclusões: Nossos resultados demonstram que as respostas oxidante e inflamatória estão aumentadas desde o início do DM1, mas mantêm-se inalteradas durante a progressão da doença, sugerindo que as mudanças funcionais envolvidas nas complicações diabéticas podem começar nos primeiros anos após o diagnóstico. Arq Bras Endocrinol Metab. 2012;56(7):441-8 Descritores Diabetes tipo 1; progressão da doença; estresse oxidativo; capacidade oxidante do plasma

Type 1 diabetes mellitus: Complex interplay of oxidative stress, cytokines, gastrointestinal motility and small intestinal bacterial overgrowth

“…Controversial results are available in literature for this aspect as in one study none of the variables of lipid peroxidation showed any significant difference between T1DM patients and healthy controls while other studies in literature support the above findings. In the study by Reis et al, 2012 significant increase was detected in ROS and IL‐6 in patients with T1DM as compared to healthy subjects. In another study, children with type 1 diabetes demonstrated increased oxidative stress (lower SOD and GSH) when compared with healthy children) but their oxidative DNA damage (measured as DNA strand breaks) was not substantially altered compared with normal.…”

“…Results of present study are in similar trend as seen in the literature. One study by Reis et al, 2012 showed higher levels of IL‐6 in 42 T1DM patients than in 35 controls. In another study by He et al, 2014, the authors studied the levels of IL‐6, IL‐10 and TNF‐α in 35 children of T1DM and in 30 controls.…”

Diabetic myopathy: impact of diabetes mellitus on skeletal muscle progenitor cells

“…Chronic elevations of circulating Interleukin-6 (IL-6) are observed in T1DM and T2DM (Pradhan et al, 2001; Reis et al, 2012). While transient increases in IL-6 are associated with SC proliferation (Toth et al, 2011), chronically elevated IL-6 is correlated with significant decrements in muscle health (e.g., cancer cachexia; Roubenoff, 1997; McKay et al, 2013).…”

Elevated IFN-alpha/beta levels in a streptozotocin-induced type I diabetic mouse model promote oxidative stress and mediate depletion of spleen-homing CD8+ T cells by apoptosis through impaired CCL21/CCR7 axis and IL-7/CD127 signaling

“…ACCEPTED MANUSCRIPT 21 immune complications [59][60][61]. Therefore, diabetes is accompanied by high free radical levels in the blood and spleen (Fig.…”