Oxidative stress and mitochondrial impairment mediated apoptotic cell death induced by terpinolene in <i>Schizosaccharomyces pombe</i>

Ağuş, Hızlan Hıncal; Sarp, Cemaynur; Cemiloglu, Meryem

doi:10.1039/c8tx00100f

Cited by 25 publications

(16 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Upon analysis of the composition of the extract, Terpinolene was found to be the major compound. Terpinolene is one of the most abundant monoterpenes, which is known for its sedative [8] , antifungal [49] , anticancer, antioxidant [6], apoptotic [50] activities, as well anti-inflammatory and anti-nociceptive activities in association with diclofenac [7]. Interestingly, terpinolene, which is a main constituent of the essential oil of Protium heptaphyllum , exhibited an anti-mutagenic activity, suggesting its potential use as a chemo-preventive agent for cancer [51].…”

Section: Discussionmentioning

confidence: 99%

The pro-apoptotic effect of a Terpene-rich Annona cherimola leaf extract on leukemic cell lines

Ammoury

Younes

Khoury

et al. 2019

BMC Complement Altern Med

View full text Add to dashboard Cite

BackgroundThe edible fruit Annona cherimola has previously shown many nutritional and medicinal properties. The current study evaluates the anti-cancer and anti-proliferative properties of Annona cherimola ethanolic leaf extract (AELE) on Acute Myeloid Leukemia (AML) cell lines cultured in vitro (Monomac-1 and KG-1).MethodsThe anti-proliferative effect of A. cherimola ethanolic leaf extract was evaluated via cell viability assay. Its pro-apoptotic effect was assessed through Cell Death ELISA and dual Annexin V/PI staining. To further investigate the molecular mechanism by which the extract promoted apoptosis and inhibited the proliferation of the AML cells used, apoptotic protein expression was determined through western blots. Extract composition was elucidated by Gas Chromatography-Mass Spectrometry (GC-MS).ResultsOur results showed that the treatment with A. cherimola ethanolic leaf extract exhibited an inhibitory effect on the proliferation of both cancer cell lines used in a dose- and time-dependent manner, with no toxic effects on normal mononuclear cells (MNCs) isolated from human bone marrow. This effect was mediated by DNA fragmentation and apoptosis, as revealed by Cell Death ELISA and dual Annexin V/PI staining. Western blot analysis revealed a Bax/Bcl2 dependent mechanism of apoptosis, as well as PARP cleavage, confirming the apoptotic results observed previously. These effects may be attributed to the presence of terpenes which constitute a large component of the leafy extract, as revealed via GC-MS.ConclusionAll the data presented in our study show that the terpene-rich A. cherimola ethanolic leaf extract exhibits an anti-proliferative and pro-apoptotic effect on the AML cell lines used.

show abstract

Section: Discussionmentioning

confidence: 99%

The pro-apoptotic effect of a Terpene-rich Annona cherimola leaf extract on leukemic cell lines

Ammoury

Younes

Khoury

et al. 2019

BMC Complement Altern Med

View full text Add to dashboard Cite

show abstract

“…However, the molecular basis has not been explained clearly and comprehensively. Responses of several phytopathogens to the specific antifungals have been investigated by several molecular genetics approaches such as the gene expression analysis, the oxidative stress and the apoptosis determination (Agus et al, 2018;Yörük et al, 2018a). However, those investigations present only limited data for antifungal resistance.…”

Section: Discussionmentioning

confidence: 99%

Tetraconazole Leads to Alterations in Fusarium Graminearum at Different Molecular Levels

Yörük¹

2018

Appl. Ecol. Env. Res.

View full text Add to dashboard Cite

The alterations in F. graminearum due to tetraconazole (TCZ) have been investigated in this study. The minimum inhibitory concentration (MIC), and inhibitory concentrations 50% and 25% (IC 50 and IC 25 ) were obtained by adding different concentrations of TCZ to potato dextrose agar (PDA). MIC, IC 50 and IC 25 values were detected as 32, 16 and 8 µg/mL TCZ. Epigenetic changes have been evaluated via genomic template stability (GTS) by the RAPD and CRED-RA methods. GTS values were recorded as 86.73% and 85.71% in IC 50 and IC 25 sets. A total of 157 bands were obtained via RAPD. The average % polymorphism values of HapII and MspI digested samples were detected as 5.72 and 5.37 with 4.68 and 2.6% for IC 25 and IC 50 groups, respectively. The expression levels of genes related to apoptosis (Hog1), cell stability (Mgv1), oxidative stress (POD) and deoxynivalenol production (tri5) in the control and the experiment sets were investigated by qPCR. Increased concentrations of TCZ lead to upregulation in Hog1, Mgv1 and POD genes whereas down regulation was recorded in tri5 expression. The late apoptosis and oxidative stress were detected via the acridine orange-ethidium bromide (AoEb) and the DCF-DA staining assays. The findings showed that TCZ could lead to damage on phytopathogenic fungi at genomic, epigenetics, transcriptomics and apoptotic levels.

show abstract

“…When acridine orange and ethidium bromide are used at the same time, fragmented bright red-orange cell nuclei will indicate (dead) late apoptotic cells, whereas live cells are only stained by acridine orange and seen as brightgreen cells. This experiment was performed as indicated previously (Pajaniradje et al, 2014;Agus et al, 2018). Briefly, cells were mixed with 5 µL of acridine orange/ ethidium bromide solution (60 µg/mL of AO:100 µg/mL of EB, dissolved in PBS).…”

Section: Acridine Orange/ethidium Bromide (Ao/eb) Dual Assaymentioning

confidence: 99%

Crosstalk between autophagy and apoptosis induced by camphor in Schizosaccharomyces pombe

Ağuş¹,

Yilmaz²,

Şengöz³

2019

Turk J Biol

Self Cite

View full text Add to dashboard Cite

Introduction Terpenes and terpenoids are herbal extracts extracted from resins of medicinal and aromatic plants including Citrus, Lamiaceae, and Pinaceae, or produced from marine organisms such as Spongia (Gershenzon and Dudareva, 2007). Many terpenes are defined as harmful chemicals having prooxidant, cytotoxic, genotoxic, and neurotoxic effects (Chueca et al., 2014; Sharma et al., 2019), whereas some other studies (Bastaki et al., 2018; Askari and Shafiee-Nick, 2019) showed antioxidant, antiinflammatory, and cytoprotective effects. Camphor (1,7,7-trimethylbicyclo[2.2.1]heptan-2-one), which is classified among the bicyclic monoterpenes constituted of 2 isoprene units and 10 carbon atoms, is a white-colored powder and found in the camphor tree and other aromatic and medicinal plants (Frizzo et al., 2000). Camphor was reported to show cytotoxicity characterized by oxidative stress, mitochondrial disruption, apoptosis and/or necrosis, and suppression of cell proliferation at high concentrations (>50 µg/L) in rat thymocytes (Cherneva et al., 2012), human colon cancer cell lines (Itani et al., 2008), and fungi (Agus et al., 2019). In addition, glutathione S-transferase, cytochrome b5, and aryl hydrocarbon hydroxylase activities were increased in response to a 300 mg/kg camphor/body weight dose in mouse liver (Banerjee et al., 1995). Although the dose-dependent and tissue-specific cytotoxic and/or cytoprotective effects of camphor were defined in different organisms and various types of mammalian cell lines (Jeon et al., 2014; Sedaghat and Torshizi, 2017; Sokolova et al., 2018), potential anticancer activities, together with mechanisms of action, are under debate and should be unraveled. The characteristics of fission yeast (Schizosaccharomyces pombe) that are analogous to mammals (Lin and Austriaco, 2014; Koyama et al., 2017, including mitochondrial biogenesis, cell cycle control, and evolutionarily conserved programmed/regulated cell death, in addition to a small and easily manipulated genome (Wood et al., 2002), make this yeast species an excellent model organism for molecular biology, biochemistry, and genetics studies (Hagan et al., 2016; Gerganova et al., 2019). Besides, fission yeast provides a valuable opportunity to be used in cancer research, for its high proliferation rate resembles the Crabtree and Warburg effect (reprogrammed energy metabolism of cancer cells) (Flores et al., 2000; Carmona

show abstract

Oxidative stress and mitochondrial impairment mediated apoptotic cell death induced by terpinolene in Schizosaccharomyces pombe

Abstract: Terpinolene induces apoptotic cell death via oxidative stress and mitochondrial impairment.

Cited by 25 publications

References 54 publications

The pro-apoptotic effect of a Terpene-rich Annona cherimola leaf extract on leukemic cell lines

The pro-apoptotic effect of a Terpene-rich Annona cherimola leaf extract on leukemic cell lines

Tetraconazole Leads to Alterations in Fusarium Graminearum at Different Molecular Levels

Crosstalk between autophagy and apoptosis induced by camphor in Schizosaccharomyces pombe

Contact Info

Product

Resources

About