Oxidative stress and phenotype frequencies of paraoxonase‐1 in teratozoospermia

Abstract

Oxidative stress causes infertility in men by affecting especially sperm morphology.The aim of the study was to examine the frequencies of paraoxonase-1 (PON1) phenotypes and the serum PON1, arylesterase, total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index, catalase and thiol levels in teratozoospermic infertile men and normospermic fertile men. The study included 184 teratozoospermic infertile men and 72 normospermic fertile men. The double substrate method was employed to determine the phenotype distribution of PON1. The evaluation of sperm morphology was made in accordance with the Kruger's criteria. TAS, catalase and thiol levels were determined to be significantly lower in teratozoospermic infertile men compared to normospermic fertile men. A significant change was not observed in the levels of TOS, PON1 and arylesterase. There was a positive correlation between catalase and thiol levels and sperm morphology. While there were significantly more teratozoospermic men with AA phenotypes compared to normospermic men, there were significantly more persons with AB and BB phenotypes in normospermic men than in teratozoospermic men. As far as we know, such a study was conducted for the first time and suggests that PON1 phenotypic distribution may play a significant role in sterile males because of impaired sperm morphology. K E Y W O R D Soxidative stress, paraoxonase 1, PON1 phenotype, teratozoospermia

High-Risk HPV with Multiple Infections Promotes CYP2E1, Lipoperoxidation and Pro-Inflammatory Cytokines in Semen of Asymptomatic Infertile Men

“…As the role of the sperm tail is to produce motility, alterations decrease sperm motility [ 33 ]. However, sperm motility was not reduced in conditions of OE, as has been described in other reports [ 34 ]. One possible explanation is that the subjects explored here were young and probably have better resistance to oxidation, as it has been demonstrated that the percentage of motile spermatozoa decreases with age [ 35 ].…”

“…One possible explanation is that the subjects explored here were young and probably have better resistance to oxidation, as it has been demonstrated that the percentage of motile spermatozoa decreases with age [ 35 ]. Moreover, lifestyle practices linked with antioxidant protection in this population and the existence of additional non-studied mechanisms that connect the activity of OE and motility, such as peroxiredoxins, glucose 6-phosphate dehydrogenase and paraoxonase-1 activity, which also have important roles in antioxidant protection [ 34 , 35 ], could explain this result.…”

Paraoxonase 1 activity in the sperm-rich portion of boar ejaculates is positively associated with sperm quality

“…Such individual variation may reflect the fact that PON1 activity is influenced by genetic polymorphisms, environmental and dietary factors, as recently reviewed by Ponce-Ruiz et al (2020). Also, it was recently demonstrated that phenotypic distribution can affect seminal plasma PON1 activity in men (Ozer et al, 2019).…”

“…Due to its associations with reproductive functions in various species, PON1 has been considered as a potential fertility biomarker. Reduced PON1 activity was observed in the seminal plasma of men presenting teratozoospermia, azoospermia, subfertility and infertility (Verit et al, 2009;Gulum et al, 2017;Ozer et al, 2019), whereas increased PON1 activity was positively associated with sperm concentration (Ozer et al, 2019). In bulls, PON1 activity in the seminal plasma presented positive correlations with a subjective indicator of sperm concentration and with several parameters of sperm quality (Ferreira et al, 2018).…”

Association of PON1-L55M Genetic Variation with Idiopathic Male Infertility in an Iranian Population: A Case-Control Study

“…It has been documented that the ability of HDL to prevent both the oxidation of LDL and the interaction between macrophages and endothelium by inactivation of PON1 increases [27,28]. Several researchers have reported that PON1 is present at various stages of spermatogenesis however the exact role of PON1 in male reproductive system is unknown [21,25]. More than seven polymorphisms in the coding region and ve in promoter region have been identi ed in the PON1 gene.…”