Oxidative stress, antioxidant status and DNA damage in patients with impaired glucose regulation and newly diagnosed Type 2 diabetes

Song, Fang; Jia, Wenbo; Yao, Ying; Hu, Yafei; Lei, Lin; Lin, Jie; Sun, Xiufa; Liu, Liegang

doi:10.1042/cs20060323

Cited by 160 publications

(127 citation statements)

References 42 publications

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“…19 Our results showed an inverse correlation of FBS with TAC. These results are in consistent with Akinosun et al 20 and Song et al 21 in poorly controlled Type 2 diabetics associated with a defect in antioxidant defense of the body against oxidative stress.…”

Section: 18supporting

confidence: 82%

Type 2 Diabetes Mellitus; Homocysteine, Total Anti-Oxidant Levels and Vitamins in Patients of Different Duration

Khawaja¹,

Sarwar²,

Bloach

et al. 2016

TPMJ

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Out of 120 selected subjects, 30 were assigned as control, (group 1) and 90 of DMT2. Based on duration, patients of DMT2 were divided into; group 2 (DMT2 <5 years), 3 (DMT2 = 5-10 years) and group 4 (DMT2 >10 years). Smokers, renal failure, coronary artery disease, thyroid disease and patients on antioxidant treatment were excluded from the study. DMT2 was diagnosed according to American Diabetes Association standards. The fasting plasma glucose levels were measured by glucose oxidase method; HbA1c by automated kit, TAC by calorimetric TAC Assay Kit (BioVision), Vitamin C and E by ELISA Kit (HUMAN) while homocysteine measured by AXSYM HCY assay kit (ABBOTT). Cut off values for HbA1c was taken as ≤6%; FBS ≤110 mg/dl; TAC ≥1.16 mmol/L; Vitamin C ≥2 mg/dl; Vitamin E ≥ 9.5nmol/ml and homocysteine was < 6.3 μmol/L. Results: As the duration of DMT2 increases, levels of vitamin C and TAC fall significantly (p <0.05) in all groups except between groups 1 & 2; however, vitamin E, decreased significantly in all the groups with increased DMT2 duration. A significantly increased level of HbA1c was noticed in groups 2, 3 and 4 compared to group 1 with increased DMT2 duration. The fasting blood sugar increased significantly in all the groups except between group 3 and 4. ANOVA showed significant differences (p <0.05) between each group and within the groups when Hb1Ac, vitamin E, vitamin C, & TAC were compared. A positive significant correlation was observed when HbA1c was correlated with FBS; TAC correlated with vitamin C and E and between vitamin C and vitamin E. Conclusions: The levels of TAC, vitamin C and E gradually decrease with increased DMT2 duration; so should be supplemented in diabetics. TAC status can be taken as early marker to detect complications while homocysteine levels to prevent diabetic complications. Key words:Homocysteine, Total Anti-Oxidant, Vitamins, Diabetes Mellitus Diabetes Mellitus type-2, Homocystein, Anti-oxidants Article Citation: Khawaja R, Sarwar M, Bloach MH, Ahmad I, Munir TA. Type 2 diabetes mellitus; homocysteine, total anti-oxidant levels and vitamins in patients of different duration. Professional Med J 2016;23(9)

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Section: 18supporting

confidence: 82%

Type 2 Diabetes Mellitus; Homocysteine, Total Anti-Oxidant Levels and Vitamins in Patients of Different Duration

Khawaja¹,

Sarwar²,

Bloach

et al. 2016

TPMJ

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“…Increased oxidative stress is also a major feature of Type II diabetes, a major risk factor for atherosclerosis [13]. For example, circulating mononuclear cells from patients with type II diabetes exhibit increased production of reactive oxygen species [14] and enhance lipid peroxidation [15] resulting in DNA damage [16] and mitochondrial dysfunction [17].…”

Section: Oxidative Stress (Reactive Oxygen Species)mentioning

confidence: 99%

Role of DNA damage in atherosclerosis—Bystander or participant?

Gray

Bennett

2011

Biochemical Pharmacology

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“…Studies conducted among different populations including Ghanaian, Nigerian, Chinese, Italian, and Indian have also reported a decrease in TAS in Type 2 diabetes mellitus patients when compared to controls [24][25][26][27][28]. However, there also certain contradictory results suggesting an increase in TAS in patients with diabetes [29].…”

Section: Association Between Tas and Type 2 Diabetes Mellitusmentioning

confidence: 99%

Assessment of Dna Strand Breaks and Total Antioxidant Status in Type 2 Diabetic Patients With and Without Complications- A Case-Control Study

Nithya

Isabel²,

Angeline³

et al. 2017

Asian J Pharm Clin Res

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Objective: To evaluate the total antioxidant status (TAS) and the extent of DNA strand breaks (damage) as a measure of oxidative stress biomarkers in patients with Type 2 diabetes (with and without complications) and controls.Methods: Blood samples were collected from 200 patients with Type 2 diabetes mellitus (n=100, with complications and n=100, without complications) and 100 healthy individuals. Oxidative DNA damage was evaluated using alkaline single cell gel electrophoresis (comet assay). TAS was assessed by ferric reducing ability of plasma assay.Results: TAS was found to be significantly lower in patients with Type 2 diabetes (with and without complications) compared to controls (p<0.001). Similarly, patients with complications of Type 2 diabetes mellitus had significantly lower TAS when compared to patients with diabetes (p=0.007). DNA damage analysis showed that the extent of damage was high in patients with diabetes mellitus (with and without complications) compared to controls (p<0.001). Fasting glucose and glycosylated hemoglobin level (HbA 1C ) were found to be significantly higher in patients with diabetes than controls (p<0.05). Correlation analysis showed that there is no association between age, duration, sugar level, HbA 1C , TAS, and DNA damage in patients with Type 2 diabetes mellitus. Conclusion:Alterations in TAS and the extent of DNA damage were observed in patients with complications of diabetes mellitus indicate that oxidative stress is more in patients with complications when compared to patients without complications and healthy individuals. Therefore, further DNA damage and onset of complications in Type 2 diabetes mellitus could be prevented by counteracting the oxidative stress by therapeutic interventions using appropriate antioxidants.

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Oxidative stress, antioxidant status and DNA damage in patients with impaired glucose regulation and newly diagnosed Type 2 diabetes

Cited by 160 publications

References 42 publications

Type 2 Diabetes Mellitus; Homocysteine, Total Anti-Oxidant Levels and Vitamins in Patients of Different Duration

Type 2 Diabetes Mellitus; Homocysteine, Total Anti-Oxidant Levels and Vitamins in Patients of Different Duration

Role of DNA damage in atherosclerosis—Bystander or participant?

Assessment of Dna Strand Breaks and Total Antioxidant Status in Type 2 Diabetic Patients With and Without Complications- A Case-Control Study

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