Oxidative stress, apoptosis and histopathological alterations in brain stem and diencephalon induced by subacute exposure to fipronil in albino rats

Awad, Mohamed A.; Ahmed, Zainab Sabry Othman; AbuBakr, Huda O.; Elbargeesy, Gehad Abd El‐Fattah Hassan; Moussa, Moukhtar Hanafi Gad

doi:10.1007/s11356-021-15537-3

Cited by 7 publications

(4 citation statements)

References 54 publications

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“…This increase may result from oxidative degradation of polyunsaturated fatty acids due to lipid peroxidation that disturbs the membrane structurefunction [36], induces oxidative modification of membrane-bound proteins, and increases the level of protein carbonyl [37]. This hypothesis is in a line with the study of Awad et al [38], who showed an increase in MDA and protein carbonyl levels in the brain tissues after intoxication with FPN. Additionally, FPN might elevate the brain activities of SOD, GPx, and CAT due to the protective effect of these enzymes.…”

Section: Discussionsupporting

confidence: 74%

Garlic and allopurinol alleviate the apoptotic pathway in rats’ brain following exposure to fipronil insecticide

Bakr

Desouky

Ibrahim

2022

Environ Anal Health Toxicol

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Fipronil can cause oxidative tissue damage and apoptosis. Our goal is to evaluate the antiapoptotic impact of garlic or allopurinol against fipronil neurotoxicity. Thirty-six mature male albino rats were separated into control, garlic aqueous extract (500 mg/kg), allopurinol (150 mg/L in their drinking water), fipronil (13.277 mg/kg), garlic+fipronil, and allopurinol+fipronil. Our results revealed that fipronil induced a significant increase in brain malondialdehyde, protein carbonyl levels as well as enzymatic antioxidant activities (superoxide dismutase, catalase, glutathione peroxidase, and xanthine oxidase), but glutathione-S-transferase recorded a significant decrease as compared to the control. In addition, fipronil significantly up-regulated the brain pro-apoptotic (Bax) and caspase -3 mRNA gene expression and induced DNA fragmentation but caused down-regulation in anti-apoptotic (Bcl-2) mRNA genes expression. Interestingly, co-administration with garlic or allopurinol improved the lipid peroxidation, antioxidant disturbance, and apoptosis induced by fipronil in the brain tissues. In conclusion, garlic or allopurinol reduced fipronil-induced apoptosis and reduced oxidative tissue damage, most likely through enhancing the tissue antioxidant defense system.

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Section: Discussionsupporting

confidence: 74%

Garlic and allopurinol alleviate the apoptotic pathway in rats’ brain following exposure to fipronil insecticide

Bakr

Desouky

Ibrahim

2022

Environ Anal Health Toxicol

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“…Fipronil is a fairly new phenylpyrazole insecticide that is widely used in many fields such as agriculture, veterinary medicine, and public health. 31 Fipronil or its metabolites cause neurotoxicity by suppressing the inhibitory effect of gamma aminobutyric acid (GABA) by targeting chloride channels regulated by GABAA. 32 In addition to this, fipronil causes toxicity in many tissues and organs through oxidative stress because of excessive accumulation of reactive oxygen species (ROS) in cells and disruption of antioxidant and oxidant system balance.…”

Section: Discussionmentioning

confidence: 99%

The ameliorative effects of quercetin and curcumin against subacute nephrotoxicity of fipronil induced in Wistar rats

Uzunhisarcıklı

Apaydın

Baş

et al. 2023

Toxicology Research

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Fipronil is a phenylpyrazole insecticide that is widely used in agricultural, veterinary, and public health fields for controlling a wide variety of insect species and it is an environmentally potent toxic substance. Curcumin and quercetin, which are well-known natural antioxidants, are widely used to prevent the harmful effects of free radicals on biological systems. The present study aimed to determine the potential ameliorative effects of quercetin and/or curcumin on fipronil-induced nephrotoxicity in rats. Curcumin (100 mg/kg of body weight), quercetin (50 mg/kg of body weight), and fipronil (3.88 mg/kg of body weight) were administered to male rats by intragastric gavage for 28 consecutive days. In the present study, body weight, kidney weight, the renal function markers (blood urea nitrogen, creatinine, and uric acid levels) in the blood, antioxidant enzyme activities, and malondialdehyde level as markers of oxidative stress, and histological changes of the renal tissue were evaluated. The levels of serum blood urea nitrogen, creatinine, and uric acid were significantly increased in fipronil-treated animals. Additionally, while superoxide dismutase, catalase, glutathione-S-transferase, and glutathione peroxidase activities were decreased in the kidney tissue of rats treated with fipronil, malondialdehyde level was significantly increased. Histopathological analyses showed that the glomerular and tubular injury occurred in the renal tissue of fipronil-treated animals. Also, the supplementation of quercetin and/or curcumin with fipronil significantly improved fipronil-induced alterations in renal function markers, antioxidant enzyme activities, malondialdehyde levels, and histological features of renal tissue.

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“…The most enriched GOs were categorized into biological processes (BP), cellular components (CC), and molecular functions (MF), which mainly included cell growth, proliferation, differentiation, apoptosis, stress response, and activation of genes involved in gene transcription ( Figure 1C ). Apoptosis and stress response lead to infiltration of immune cells in the body ( 16 ). And cell growth, proliferation and apoptosis all exhibit circadian rhythms ( 17 ).…”

Section: Resultsmentioning

confidence: 99%

“…Meanwhile, we used two machine learning algorithms to further define circadian rhythm-related signature genes. Next, given that IH is one of the most important pathophysiology of OSA disease as well as most of the basic studies of OSA disease currently use the IH model (14,15), we chose to use IH rats to simulate OSA disease. qPCR experiments verified the expression of key genes in OSA, which were all of good value for the diagnosis of OSA and closely associated with immune cell infiltration in OSA.…”

Section: Introductionmentioning

confidence: 99%

New insights from integrated bioinformatics analysis: the role of circadian rhythm disruption and immune infiltration in obstructive sleep apnea disease

Zhang,

Wang,

Pan

et al. 2023

Front. Immunol.

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BackgroundCircadian rhythm disruption and immune infiltration are both closely associated with the development of Obstructive sleep apnea (OSA) disease and a variety of cardiovascular and neurological complications, but their interactions with OSA disease are not clear. In this study, we used bioinformatics to investigate the roles of circadian rhythm disruption and immune microenvironments in OSA.MethodsWe analyzed differential genes and their associated functional pathways in the circadian rhythm-associated OSA dataset, then regrouped OSA samples using the differential genes and explored differences in immune cell infiltration between the two different subgroups. Meanwhile, we used two machine learning algorithms to further define circadian rhythm-related signature genes and to explore the relationship between key genes and immune cell infiltration. Finally, we searched for the transcription factors of the key differential gene JUN.ResultsWe screened 15 circadian rhythm-related differential genes in the OSA-related dataset and further defined 3 signature genes by machine learning algorithms. Immunoassays showed a significant increase in resting mast cell infiltration and a decrease in monocyte infiltration in the OSA group. The results of our animal experiments also confirmed that the expression of these 3 key genes, as well as the immune cell infiltration, showed a trend consistent with the results of the bioinformatics analysis.ConclusionsIn conclusion, this study reveals the interaction between circadian rhythm disruption and immune infiltration in OSA, providing new insights into the potential pathogenesis of OSA.

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Oxidative stress, apoptosis and histopathological alterations in brain stem and diencephalon induced by subacute exposure to fipronil in albino rats

Cited by 7 publications

References 54 publications

Garlic and allopurinol alleviate the apoptotic pathway in rats’ brain following exposure to fipronil insecticide

Garlic and allopurinol alleviate the apoptotic pathway in rats’ brain following exposure to fipronil insecticide

The ameliorative effects of quercetin and curcumin against subacute nephrotoxicity of fipronil induced in Wistar rats

New insights from integrated bioinformatics analysis: the role of circadian rhythm disruption and immune infiltration in obstructive sleep apnea disease

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