Oxidative stress as a component of chromium-induced cytotoxicity in rat calvarial osteoblasts

Fu, Jun; Lü, Xing; Chen, Yue; Tang, Li; Zhang, Qinghong; Dong, Qiang

doi:10.1007/s10565-007-9029-7

Cited by 26 publications

(10 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, treatment with 100 μM LA significantly reduced ROS compared to 50 μM LA (Table 1). According to previous research, LA seem to be an ideal antioxidant because of several of its properties, including (a) the ability to scavenge reactive oxygen species, including OH ⋅ , O 2 ⋅− , alkoxy radicals, and peroxide radicals; (b) the ability to regenerate other antioxidants such as vitamins E and C and GSH from their radical or inactive forms; and (c) metal chelating activity (Fu et al 2008;Biewenga et al 1997). The above might be possible reasons for reduction of ROS in the LA-treated irradiated murine fibroblasts.…”

Section: Discussionmentioning

confidence: 99%

Radioprotective effect of dl-α-lipoic acid on mice skin fibroblasts

et al. 2008

View full text Add to dashboard Cite

During the course of cancer radiation treatment, normal skin invariably suffers from the cytotoxic effects of gamma-radiation and reactive oxygen species (ROS), which are generated from the interaction between radiation and the water molecules in cells. The present study was designed to investigate the radioprotective role of alpha-lipoic acid (LA), an antioxidant on murine skin fibroblasts exposed to a single dose of 2, 4, 6, or 8Gy gamma-radiation. Irradiation of fibroblasts significantly increased ROS, nitric oxide, and lipid peroxidation (P < 0.001); all of these factors substantially decreased with 100 microM LA treatment. Hydroxyl radical (OH(.)) production from 8Gy irradiated fibroblasts was measured directly by electron spin resonance using spin-trapping techniques. LA was found to inhibit OH(.) production at 100-microM concentrations. Dose-dependent depletion of antioxidants, such as catalase and glutathione reductase, was observed in irradiated fibroblasts (P < 0.001), along with increased superoxide dismutase (P < 0.001). LA treatment restored antioxidant levels. Concentration of the pro-inflammatory cytokine IL-1beta was significantly reduced in irradiated fibroblasts when treated with LA. MTT and lactate dehydrogenase assays demonstrated that LA treatment reduced cell injury and protected cells against irradiation-induced cytotoxicity. Thus, we conclude that results are encouraging and need further experiments to demonstrate a possible benefit in cancer patients and the reduction of harmful effects of radiation therapy.

show abstract

Section: Discussionmentioning

confidence: 99%

Radioprotective effect of dl-α-lipoic acid on mice skin fibroblasts

et al. 2008

View full text Add to dashboard Cite

show abstract

“…ALP activity on the 9th day after osteogenic induction culture was measured by using a colorimetric endpoint assay method [22]. Total cellular ALP activity was measured using an ALP activity assay kit and was expressed as absorbance value at 520 nm.…”

Section: Methodsmentioning

confidence: 99%

The Importance of the Prenyl Group in the Activities of Osthole in Enhancing Bone Formation and Inhibiting Bone ResorptionIn Vitro

Zhai

Pan

Niu

et al. 2014

International Journal of Endocrinology

View full text Add to dashboard Cite

Osteoporosis treatment always aimed at keeping the balance of bone formation and bone resorption. Recently, prenyl group in natural products has been proposed as an active group to enhance the osteogenesis process. Osthole has both the prenyl group and bone-protective activities, but the relationship is still unknown. In this study we found that osthole exerted a potent ability to promote proliferation and osteogenic function of rat bone marrow stromal cells and osteoblasts, including improved cell viability, alkaline phosphatase activity, enhanced secretion of collagen-I, bone morphogenetic protein-2, osteocalcin and osteopontin, stimulated mRNA expression of insulin-like growth factor-1, runt-related transcription factor-2, osterix, OPG (osteoprotegerin), RANKL (receptor activator for nuclear factor-κB ligand), and the ratio of OPG/RANKL, as well as increasing the formation of mineralized nodules. However, 7-methoxycoumarin had no obvious effects. Osthole also inhibited osteoclastic bone resorption to a greater extent than 7-methoxycoumarin, as shown by a lower tartrate-resistant acid phosphatase activity and lower number and smaller area of resorption pits. Our findings demonstrate that osthole could be a potential agent to stimulate bone formation and inhibit bone resorption, and the prenyl group plays an important role in these bone-protective effects.

show abstract

“…In the first instance, intracellular ROS concentrations were determined using 2′,7′-dichlorofluorescein (DCFH) according to a protocol described elsewhere [17]. Once inside the cell, this molecule is cleaved by endogenous esterases and can no longer pass out of the cell membrane.…”

Section: Isolated Adipocytes Measurementsmentioning

confidence: 99%

Glucose and insulin modify thrombospondin 1 expression and secretion in primary adipocytes from diet-induced obese rats

et al. 2011

View full text Add to dashboard Cite

Thrombospondin 1 (TSP-1), an anti-angiogenic factor and TGF-β activity regulator, has been recently recognized as an adipokine that correlates with obesity, inflammation and insulin-resistance processes. In the present study, epididymal adipocytes of rats that were fed a chow (C) or a high fat diet (HFD) for 50 days, were isolated and incubated (24-72 h) in low (LG; 5.6 mM) or high (HG; 25 mM) glucose, in presence or absence of 1.6 nM insulin. Rats fed the HF diet showed an established obesity state. Serum TSP-1 levels and TSP-1 mRNA basal expression of adipocytes from HFD rats were higher than those from controls. Adipocytes from HFD animals presented an insulin-resistance state, as suggested by the lower insulin-stimulated glucose uptake as compared to controls. TSP-1 expression in culture was higher in adipocytes from obese animals at 24 h, but when the adipocytes were treated with HG, these expression levels dropped dramatically. Later at 72 h, TSP-1 expression was lower in adipocytes from HFD rats, and no effects of the other treatments were observed. Surprisingly, the secretion levels of this protein at 72 h were increased significantly by the HG treatment in both types of adipocytes, although they were even higher in adipocytes from obese animals. Finally, cell viability was significantly reduced by HG treatment in both types of adipocytes. In summary, TSP-1 expression/secretion was modulated in an in vitro model of insulinresistant adipocytes. The difference between expression and secretion patterns suggests a post-transcriptional regulation. The present study confirms that TPS-1 is closely associated with obesity-related mechanisms.

show abstract

Oxidative stress as a component of chromium-induced cytotoxicity in rat calvarial osteoblasts

Cited by 26 publications

References 60 publications

Radioprotective effect of dl-α-lipoic acid on mice skin fibroblasts

Radioprotective effect of dl-α-lipoic acid on mice skin fibroblasts

The Importance of the Prenyl Group in the Activities of Osthole in Enhancing Bone Formation and Inhibiting Bone ResorptionIn Vitro

Glucose and insulin modify thrombospondin 1 expression and secretion in primary adipocytes from diet-induced obese rats

Contact Info

Product

Resources

About