Oxidative Stress Biomarkers and Erythrocytes Hemolysis in Well-Trained Equine Athletes Before and After Exercise

Andriichuk, Anastasiia; Tkachenko, Halyna; Tkachova, Irina

doi:10.1016/j.jevs.2015.09.011

Cited by 23 publications

(10 citation statements)

References 30 publications

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“…It was also hypothesised that decreases in seminal concentration could be caused by increases of seminal reactive oxygen species (ROS) following exercise [37,40]. Although ROS damage of spermatozoa with regards to seminal processing has been researched [41][42][43]; limited research investigating the effects of exercise and ROS damage in equine semen is available. In humans, sufficiently elevated levels of ROS to disrupt reproductive functions are seen following exercise [40].…”

Section: Concentrationmentioning

confidence: 99%

A Preliminary Comparison of Semen Quality between Competing and Non-Competing Equine Stallions

Wilson¹,

Twigg-Flesner

2017

J Vet Sci Technol

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Rationale: Artificial insemination allows sport horse stallions within breeding programmes to breed and compete concurrently. The level of exercise of stallions complete during the breeding season is a controversial subject. Daily exercise at low intensities is important for the mental and reproductive well-being of the stallion, however higher intensities of exercise, as seen in competing stallions, may have detrimental effects on seminal quality. The purpose of this study was to gain a greater understanding into the effects of competition and discipline on equid stallion semen through analysis of seminal parameters. The identification of optimal competition management for breeding stallions may lead to increased stallion fertility and economic gain. Methods:This retrospective study evaluated the seminal data of 1130 stallion collections from two UK based stud farms between 2009 and 2015. Seminal volume, concentration and progressive motility were analysed for differences between competing and non-competing stallions, then for differences between stallion disciplines. Results:Competing stallion semen concentration and progressive motility was significantly lower than noncompeting stallions (p<0.05). Semen volume was significantly higher in competing stallions (p<0.05) than noncompeting stallions. Non-competing stallion semen count was significantly higher than that of competing stallions (p<0.05). Conclusion:The difference in semen quality between competing and non-competing stallions, as well as the difference between disciplines suggests endocrinological and physiological changes occur in relation to training intensity and competition. Further research into semen quality considering exercise and competition will allow for contextualisation as to why these differences occurred.

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Section: Concentrationmentioning

confidence: 99%

A Preliminary Comparison of Semen Quality between Competing and Non-Competing Equine Stallions

Wilson¹,

Twigg-Flesner

2017

J Vet Sci Technol

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“…In our study, we successfully validated the measurement of intraerythrocytic ROS in healthy horses with DCFH‐DA using flow cytometry. Assessing intracellular ROS levels in erythrocytes can potentially contribute to understanding the effect of free radical accumulation during exercise 18,19 and disease 20 . Since DCFH‐DA is not cell‐specific, the same technique can be easily adapted to the erythrocytes of other species or any other single‐cell suspension by adjusting the gating.…”

Section: Discussionmentioning

confidence: 99%

Validation of a flow cytometric assay to detect intraerythrocytic reactive oxygen species in horses

Serpa

Woolcock

Taylor

et al. 2021

Veterinary Clinical Pathol

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Background: Oxidative stress refers to the accumulation of reactive oxygen species (ROS). Most assays for ROS detection are costly, laborious, and usually use indirect markers. The use of 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) is a possible alternative. This substance becomes a fluorochrome when oxidized by ROS, with the resultant fluorescence proportional to ROS concentration. Erythrocytes are highly exposed to ROS, resulting in cell damage and consequently impaired oxygen delivery. The effects of this exposure in physiologic and pathologic conditions necessitate an improvement in ROS detection methods. Objective: We aimed to validate intraerythrocytic ROS detection by flow cytometry using DCHF-DA in healthy horses. Methods: Erythrocytes from 31 healthy horses were isolated, incubated with DCFH-DA, and either left unstimulated or stimulated with hydrogen peroxide (H 2 O 2). For specificity, each cellular component of blood was separated and plotted according to its size and complexity. Samples were run in triplicate for intra-assay precision and five consecutive times for inter-assay repeatability. Stability was determined by analyzing the same sample up to 48 hours after blood collection. The acceptable coefficient of variation (CV) was ≤20%. Results: The intra-assay CV was 1.7% and 13.3%, and the inter-assay CV was 4.8% and 17.8% for unstimulated and stimulated samples, respectively. Unstimulated and stimulated samples were stable for up to 48 and 24 hours, respectively. Stimulated samples had greater fluorescence than unstimulated samples (P < .0001). Conclusions: This flow cytometric assay demonstrated adequate specificity, precision, and stability and is, therefore, a promising technique with multiple applications for studying oxidative stress in horses.

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“…Нами была изучена активность ключевых ферментов распада пуриновых нуклеотидов -аде-нозиндезаминазы (АДА) и ксантиноксидазы (КО), которые способны нарабатывать АФК, а также ак-тивности ферментов первой линии антиоксидант-ного звена защиты от АФК: супероксиддисмутазы (СОД) и глутатионпероксидазы (ГПО) [4]. В качестве показателей интенсивности СРО мы исследовали уровень кетондинитрофенилгидразонов (КДНФГ) и альдегиддинитрофенилгидразонов (АДНФГ) ней-трального и основного характера, что в последнее десятилетие признано одним из наиболее ранних и стабильных показателей поражения различных тканей организма при свободно-радикальной окис-лительной модификации белков (ОМБ) [5]. Значения этих показателей в плазме крови мы определяли для изучения статуса ПОС и АОС при РЖ.…”

Section: материалы и методыunclassified

Влияние Старения На Окислительный Потенциал Плазмы Крови У Онкологических Больных

Зуйков¹,

Зинкович²

2017

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Влияние старения на окислительный потенциал плазмы крови у онкологических больныхДонецкий национальный медицинский университет им. М. ГорькогоЦель: изучить состояния про-и антиоксидантной системы, а также показатели свободно-радикального окисления белков плазмы крови у больных раком желудка в зависимости от возраста. Материалы и методы: материалом исследования послужила плазма крови 35 больных раком желудка в возрасте 40-79 лет. Изучили активность ферментов обмена пуриновых нуклеотидов -аденозиндеза-миназу (АДА) и ксантиноксидазу (КО), как показатели прооксидантной системы; антиоксидантную защи-ту -глутатионпероксидазу (ГПО) и супероксиддисмутазу (СОД); показатели окислительной модифика-ции белков (ОМБ) -2,4-динитрофенилгидразоны. Методом изучения активностей ферментов и уровня 2,4-динитрофенилгидразонов была спектрофотометрия. Результаты: установлено, что при старении организма у больных происходит усиление прооксидантного статуса плазмы крови за счет повышения активностей аденозиндезаминазы и ксантиноксидазы, а также уровня 2,4-динитрофенилгидразонов на фоне снижения активностей ферментов антиоксидантной защи-ты -супероксиддисмутазы и глутатионпероксидазы. Выводы: обнаружены разнонаправленные изменения активностей ферментативного звена антиокси-дантной защиты у больных раком желудка.Ключевые слова: возраст, прооксиданты, антиоксиданты, рак желудка, аденозиндезаминаза, ксантинок-сидаза, супероксиддисмутаза, глутатионпероксидаза.

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Oxidative Stress Biomarkers and Erythrocytes Hemolysis in Well-Trained Equine Athletes Before and After Exercise

Cited by 23 publications

References 30 publications

A Preliminary Comparison of Semen Quality between Competing and Non-Competing Equine Stallions

A Preliminary Comparison of Semen Quality between Competing and Non-Competing Equine Stallions

Validation of a flow cytometric assay to detect intraerythrocytic reactive oxygen species in horses

Влияние Старения На Окислительный Потенциал Плазмы Крови У Онкологических Больных

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