Oxidative stress biomarkers in the perinatal period: Diagnostic and prognostic value

Perrone, Serafina; Laschi, Elisa; Buonocore, Giuseppe

doi:10.1016/j.siny.2020.101087

Cited by 29 publications

(33 citation statements)

References 68 publications

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“…Awareness of these oxidative stress markers could improve our understanding of their environment and their implications for possible neonatal and perinatal pathologies [ 29 ]. Additionally, some studies suggest there is a prognostic value to these elevated oxidative stress markers, relating them to ultrasound and cardiotocographic alterations at the time of delivery [ 30 , 31 ]. In the present work, gene and protein expression was studied using real-time PCR (RT-qPCR) and immunohistochemistry of NOX-1, NOX-2 and iNOS and the presence of hypoxia markers (HIF-1α) in the umbilical cords of newborns of pregnant women with CVD.…”

Section: Introductionmentioning

confidence: 99%

Newborns of Mothers with Venous Disease during Pregnancy Show Increased Levels of Lipid Peroxidation and Markers of Oxidative Stress and Hypoxia in the Umbilical Cord

et al. 2021

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Chronic venous disease (CVD) encompasses a set of disorders of the venous system that have a high prevalence in Western societies and are associated with significant sociohealth costs. Pregnancy is a period in which different hormonal and haemodynamic changes occur that lead to significant changes in the cardiovascular system, increasing the risk of developing venous problems, especially during the third trimester of gestation. In turn, CVD involves a series of local and systemic alterations that can have negative repercussions in pregnancy. In this context, the role of oxidative stress in the pathophysiology of this condition has been shown to significantly affect other vascular structures during pregnancy, such as the placenta. However, the effects of oxidative stress on the umbilical cord in women with CVD have not yet been fully elucidated. Thus, the objective of this study was to analyse the gene and protein expression of the enzymes NOX-1, NOX-2 and iNOS, which are involved in the production of reactive oxygen and nitrogen species, respectively. Similarly, the presence of hypoxia-inducible factor 1-alpha (HIF-1α) in the umbilical cord in women with CVD was compared to that of pregnant control women, and the levels of the lipid peroxidation marker malonyldialdehyde (MDA) in cord tissue and blood was also analysed. Our results support a significant increase in the enzymes NOX-1, NOX-2 and iNOS and HIF-1α and MDA in the umbilical cord tissue and blood of women with CVD. For the first time, our work demonstrates an increase in oxidative stress and cellular damage in the umbilical cords of pregnant women who develop this condition, deepening the understanding of the consequences of CVD during pregnancy.

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Section: Introductionmentioning

confidence: 99%

Newborns of Mothers with Venous Disease during Pregnancy Show Increased Levels of Lipid Peroxidation and Markers of Oxidative Stress and Hypoxia in the Umbilical Cord

et al. 2021

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“…Repeated, brief IH episodes with resolution in either room air or hyperoxia, is frequently experienced by ELGANs. IH is associated with oxidative stress [39] and poor neonatal outcomes [40][41][42][43]. F-isoprostanes are a family of prostaglandin-like compounds that are stable biomarkers of lipid peroxidation originating from oxidation of arachidonic acid and docosahexaenoic acid [44,45], independent of the cyclooxygenase (COX) enzyme [46].…”

Section: Discussionmentioning

confidence: 99%

Dose Response of Bumetanide on Aquaporins and Angiogenesis Biomarkers in Human Retinal Endothelial Cells Exposed to Intermittent Hypoxia

et al. 2021

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Aquaporins (AQPs) are important for regulating cellular water, solute transport, and balance. Recently, AQPs have also been recognized as playing a key role in cell migration and angiogenesis. In the retina, hypoxia induces vascular endothelial growth factor (VEGF), a potent angiogenic and vascular permeability factor, resulting in retinal edema, which is facilitated by AQPs. Bumetanide is a diuretic agent and AQP 1–4 blocker. We tested the hypothesis that bumetanide suppression of AQPs ameliorates intermittent hypoxia (IH)-induced angiogenesis and oxidative stress in human microvascular retinal endothelial cells (HMRECs). HMRECs were treated with a low-dose (0.05 µg/mL) or high-dose (0.2 µg/mL) of bumetanide and were exposed to normoxia (Nx), hyperoxia (50% O2), or IH (50% O2 with brief hypoxia 5% O2) for 24, 48, and 72 h. Angiogenesis and oxidative stress biomarkers were determined in the culture media, and the cells were assessed for tube formation capacity and AQP-1 and -4 expression. Both doses of bumetanide significantly decreased oxidative stress and angiogenesis biomarkers. This response was reflected by reductions in tube formation capacity and AQP expression. These findings confirm the role of AQPs in retinal angiogenesis. Therapeutic targeting of AQPs with bumetanide may be advantageous for IH-induced aberrant retinal development.

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“…In addition, stress response proteins, ROS-forming enzymes (e.g., xanthine oxidase (XO), uncoupled nitric oxide synthases (NOS), and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX)), and the activity of antioxidant enzymes (e.g., superoxide dismutase (SOD), catalase, and glutathione peroxidase (GPX)) can be used as OS biomarkers. [ 47 ] To better understand the mechanisms underlying oxidative stress, antioxidant defense, and redox signaling, it is essential to assess the protein thiol redox state. However, this state is seldom assessed immunologically because of the inability to distinguish reduced and reversibly oxidized thiols by Western blotting [ 48 , 49 , 50 , 51 , 52 , 53 , 54 ].…”

Section: Discussionmentioning

confidence: 99%

“…These are non-enzymatic products from the oxidation of amino acid-free radicals and other PUFAs, and are chemically stable. These compounds have been detected electively in lipid-rich tissues, such as brain tissue, which, being rich in polyunsaturated lipids, is highly sensitive to oxidative stress [ 16 , 17 , 18 , 19 , 20 , 21 , 25 , 29 , 30 , 33 , 34 , 35 , 40 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 61 , 65 , 66 , 67 ].…”

Section: Discussionmentioning

confidence: 99%

Oxidative Stress in Preterm Infants: Overview of Current Evidence and Future Prospects

et al. 2020

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Preterm birth (PTB), defined as parturition prior to 37 weeks of gestation, is the leading cause of morbidity and mortality in the neonatal population. The incidence and severity of complications of prematurity increase with decreasing gestational age and birthweight. The aim of this review study is to select the most current evidence on the role of oxidative stress in the onset of preterm complication prevention strategies and treatment options with pre-clinical and clinical trials. We also provide a literature review of primary and secondary studies on the role of oxidative stress in preterm infants and its eventual treatment in prematurity diseases. We conducted a systematic literature search of the Medline (Pubmed), Scholar, and ClinicalTrials.gov databases, retroactively, over a 7-year period. From an initial 777 articles identified, 25 articles were identified that met the inclusion and exclusion criteria. Of these, there were 11 literature reviews: one prospective cohort study, one experimental study, three case-control studies, three pre-clinical trials, and six clinical trials. Several biomarkers were identified as particularly promising, such as the products of the peroxidation of polyunsaturated fatty acids, those of the oxidation of phenylalanine, and the hydroxyl radicals that can attack the DNA chain. Among the most promising drugs, there are those for the prevention of neurological damage, such as melatonin, retinoid lactoferrin, and vitamin E. The microbiome also has an important role in oxidative stress. In conclusion, the most recent studies show that a strong relationship between oxidative stress and prematurity exists and that, unfortunately, there is still little therapeutic evidence reported in the literature.

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Oxidative stress biomarkers in the perinatal period: Diagnostic and prognostic value

Cited by 29 publications

References 68 publications

Newborns of Mothers with Venous Disease during Pregnancy Show Increased Levels of Lipid Peroxidation and Markers of Oxidative Stress and Hypoxia in the Umbilical Cord

Newborns of Mothers with Venous Disease during Pregnancy Show Increased Levels of Lipid Peroxidation and Markers of Oxidative Stress and Hypoxia in the Umbilical Cord

Dose Response of Bumetanide on Aquaporins and Angiogenesis Biomarkers in Human Retinal Endothelial Cells Exposed to Intermittent Hypoxia

Oxidative Stress in Preterm Infants: Overview of Current Evidence and Future Prospects

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