Oxidative Stress Causes Vacuolar Fragmentation in the Human Fungal Pathogen Cryptococcus neoformans

Kim, Donghyeun; Song, Moonyong; Do, Eunsoo; Choi, Yoojeong; Kronstad, James W.; Jung, Woo-Hwan

doi:10.3390/jof7070523

Cited by 5 publications

(1 citation statement)

References 46 publications

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“…In the animal-pathogenic fungus C. neoformans , SOD1 is described as a cytosolic enzyme, although SOD activity is also detected in the lipid rafts of its plasma membrane (Siafakas et al, 2006 ), and vacuolar localization may occur under oxidative stress conditions (Kim et al, 2021 ). In C. neoformans , the Cu-sensing transcription factor Cuf1 regulates expression of SOD encoding genes, and a novel cytosolic isoform of SOD2 under Cu-limitation (Smith et al, 2021 ).…”

Section: Part Ii: Intracellular Rosmentioning

confidence: 99%

Basidiomycota Fungi and ROS: Genomic Perspective on Key Enzymes Involved in Generation and Mitigation of Reactive Oxygen Species

Mattila

Österman-Udd

Mali

et al. 2022

Front. Fungal Biol.

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Our review includes a genomic survey of a multitude of reactive oxygen species (ROS) related intra- and extracellular enzymes and proteins among fungi of Basidiomycota, following their taxonomic classification within the systematic classes and orders, and focusing on different fungal lifestyles (saprobic, symbiotic, pathogenic). Intra- and extracellular ROS metabolism-involved enzymes (49 different protein families, summing 4170 protein models) were searched as protein encoding genes among 63 genomes selected according to current taxonomy. Extracellular and intracellular ROS metabolism and mechanisms in Basidiomycota are illustrated in detail. In brief, it may be concluded that differences between the set of extracellular enzymes activated by ROS, especially by H2O2, and involved in generation of H2O2, follow the differences in fungal lifestyles. The wood and plant biomass degrading white-rot fungi and the litter-decomposing species of Agaricomycetes contain the highest counts for genes encoding various extracellular peroxidases, mono- and peroxygenases, and oxidases. These findings further confirm the necessity of the multigene families of various extracellular oxidoreductases for efficient and complete degradation of wood lignocelluloses by fungi. High variations in the sizes of the extracellular ROS-involved gene families were found, however, among species with mycorrhizal symbiotic lifestyle. In addition, there are some differences among the sets of intracellular thiol-mediation involving proteins, and existence of enzyme mechanisms for quenching of intracellular H2O2 and ROS. In animal- and plant-pathogenic species, extracellular ROS enzymes are absent or rare. In these fungi, intracellular peroxidases are seemingly in minor role than in the independent saprobic, filamentous species of Basidiomycota. Noteworthy is that our genomic survey and review of the literature point to that there are differences both in generation of extracellular ROS as well as in mechanisms of response to oxidative stress and mitigation of ROS between fungi of Basidiomycota and Ascomycota.

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Section: Part Ii: Intracellular Rosmentioning

confidence: 99%

Basidiomycota Fungi and ROS: Genomic Perspective on Key Enzymes Involved in Generation and Mitigation of Reactive Oxygen Species

Mattila

Österman-Udd

Mali

et al. 2022

Front. Fungal Biol.

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Measuring Stress Phenotypes in Cryptococcus neoformans

Upadhya,

Probst,

Alspaugh

et al. 2024

Methods in Molecular Biology

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New insight on tomato seed priming with Anabaena minutissima phycobiliproteins in relation to Rhizoctonia solani root rot resistance and seedling growth promotion

et al. 2023

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Cyanobacteria phycobiliproteins (PBPs) are already exploited in the food industries and for biotechnological applications but not in the agricultural field. Different concentrations (0.6 – 4.8 mg/mL) of Anabaena minutissima PBPs were applied to tomato seed to study their priming effect against the soil-borne fungal pathogen Rhizoctonia solani and in promoting plant growth. PBPs increased seedling emergence and vigour, showed activity against root rot disease (67%), and enhanced plant dry weight, length, and height. Generally, no dose effect has been observed except for dry weight (55% at 4.8 mg/mL). Seed treatment primed seeds and seedlings by leading to the activation of defence responses raising phenol (26% in hypocotyls) and flavonoid (26 and 45% in hypocotyls and epicotyls, respectively) contents and chitinase (4-fold at 2.4 and 4.8 mg/mL in hypocotyls) and β-1,3-D-glucanase (up to about 2-fold at all doses in epicotyls) activities. Micro-Attenuated Total Reflection Fourier Transform Infrared revealed changes in functional groups of primed seeds, hypocotyls and exudates released into the agar because of treatment. Protein extract from PBP-primed seedlings inhibited mycelial growth (67% for epicotyl proteins) and caused morphological alterations in hyphae. This research emphasizes the potential priming role of PBPs applied by seed treatment against soil-borne pathogens.

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Oxidative Stress Causes Vacuolar Fragmentation in the Human Fungal Pathogen Cryptococcus neoformans

Cited by 5 publications

References 46 publications

Basidiomycota Fungi and ROS: Genomic Perspective on Key Enzymes Involved in Generation and Mitigation of Reactive Oxygen Species

Basidiomycota Fungi and ROS: Genomic Perspective on Key Enzymes Involved in Generation and Mitigation of Reactive Oxygen Species

Measuring Stress Phenotypes in Cryptococcus neoformans

New insight on tomato seed priming with Anabaena minutissima phycobiliproteins in relation to Rhizoctonia solani root rot resistance and seedling growth promotion

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