Oxidative Stress Contributes to Slit Diaphragm Defects Caused by Disruption of Endocytosis

Xi, Gang; Lamba, Sajan A.; Mysh, Michael; Poulton, John S.

doi:10.1016/j.ekir.2023.11.018

Cited by 2 publications

References 56 publications

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Combined transcriptome and proteome profiling reveal cell-type-specific functions of Drosophila garland and pericardial nephrocytes

Meyer,

Bossen,

Janz

et al. 2024

Commun Biol

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Drosophila nephrocytes are specialised cells that share critical functional, morphological, and molecular features with mammalian podocytes. Accordingly, nephrocytes represent a preferred invertebrate model for human glomerular disease. Here, we established a method for cell-specific isolation of the two types of Drosophila nephrocytes, garland and pericardial cells, from animals of different developmental stages and ages. Mass spectrometry-based proteomics and RNA-Seq-based transcriptomics were applied to characterise the proteome and transcriptome of the respective cells in an integrated and complementary manner. We observed characteristic changes in the proteome and transcriptome due to cellular ageing. Furthermore, functional enrichment analyses suggested that larval and adult nephrocytes, as well as garland and pericardial nephrocytes, fulfil distinct physiological functions. In addition, the pericardial nephrocytes were characterised by transcriptomic and proteomic profiles suggesting an atypical energy metabolism with very low oxidative phosphorylation rates. Moreover, the nephrocytes displayed typical signatures of extensive immune signalling and showed an active antimicrobial response to an infection. Factor-specific comparisons identified novel candidate proteins either expressed and secreted by the nephrocytes or sequestered by them. The data generated in this study represent a valuable basis for a more specific application of the Drosophila model in analysing renal cell function in health and disease.

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Combined transcriptome and proteome profiling reveal cell-type-specific functions of Drosophila garland and pericardial nephrocytes

Meyer,

Bossen,

Janz

et al. 2024

Commun Biol

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Interplay of EGFR, JNK and ROS signalling in soma-germline communication in theDrosophilatestis

Alvarez,

Papagiannouli

2024

Preprint

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Cell communication via signalling exchange plays a pivotal role in multicellular development for building functional tissues and organs. In theDrosophilatestis, a pair of somatic cyst cells (CCs) encapsulate the germline that differentiates through close-range EGFR signalling activation. The conserved Dlg/Scrib/Lgl cortical polarity complex and clathrin-mediated endocytosis attenuate EGFR signalling in CCs and loss of their function leads to EGFR overactivation and non-autonomous death of the neighbouring germ cells. Here we show that EGFR overactivation results in upregulation of JNK and p38 signalling in CCs and ROS levels in the germ cells that are destined to die. Our data uncover a bidirectional feedback between JNK signalling and ROS who regulate each other within the CC-germline microenvironment, while reducing the levels of either JNK or ROS restores germ cell survival. This study provides a framework of how polarity and cellular trafficking regulate the output of multiple signalling responses cell-intrinsically and in adjacent cells, to coordinate tissue-specific responses and maintain homeostasis.

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Oxidative Stress Contributes to Slit Diaphragm Defects Caused by Disruption of Endocytosis

Cited by 2 publications

References 56 publications

Combined transcriptome and proteome profiling reveal cell-type-specific functions of Drosophila garland and pericardial nephrocytes

Combined transcriptome and proteome profiling reveal cell-type-specific functions of Drosophila garland and pericardial nephrocytes

Interplay of EGFR, JNK and ROS signalling in soma-germline communication in theDrosophilatestis

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