Oxidative stress, d-ROMs test, and ceruloplasmin

Colombini, Francesco; Carratelli, M; Alberti, Angelo

doi:10.3109/10715762.2015.1136063

Cited by 31 publications

(23 citation statements)

References 29 publications

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“…Ceruloplasmin (CP) activity may interfere with the detection of accurate concentrations of hydroperoxides [26]. CP interferes with the d-ROMs test, and previous studies have established the usefulness of this test for evaluating the global oxidative status of a biological sample [27]. Measuring oxidative stress using the d-ROMs test is controversial; however GH deficiency induces oxidative damage, interfering with normal endothelial function via ROS overproduction [6].…”

Section: Discussionmentioning

confidence: 99%

Effect of growth hormone replacement therapy on plasma diacron-reactive oxygen metabolites and endothelial function in Japanese patients: The GREAT clinical study

et al. 2018

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Abstract. Patients with growth hormone deficiency (GHD) have an increased risk of atherosclerosis and vascular mortality. Evidence suggests that endothelial dysfunction is involved in all stages of atherogenesis. This study examined the effect of growth hormone (GH) replacement therapy on diacron-reactive oxygen metabolites (d-ROMs) and endothelial function in Japanese patients with GHD, using peripheral arterial tonometry. This was an open-label, prospective, case-control study. Nine patients with GHD who had not previously received any GH replacement therapy were enrolled. The following parameters were evaluated at baseline (before treatment), and after 24 weeks of GH replacement therapy: endothelial function using the reactive hyperemia index (RHI; EndoPAT ® system), d-ROMs, blood pressure, and fasting lipid levels. Plasma GH and insulin-like growth factor-1 (IGF-1) levels were measured at baseline and after 24 weeks of GH replacement therapy. We also enrolled eight controls with pituitary disease but no GH deficiency. Over 24 weeks of GH replacement therapy, the serum IGF-1 levels normalized with significant improvement in the RHI (from 1.65 ± 0.33 to 1.92 ± 0.26, p < 0.05) and decreased d-ROM levels (from 356.8 ± 64.1 to 303.1 ± 43.3 U.CARR, p < 0.05). There were no significant improvements in the RHI or d-ROM levels in controls. GH replacement therapy in Japanese patients with GHD may be mediated by the reduced oxidative stress and the d-ROMs associated with the treatment.

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Section: Discussionmentioning

confidence: 99%

Effect of growth hormone replacement therapy on plasma diacron-reactive oxygen metabolites and endothelial function in Japanese patients: The GREAT clinical study

et al. 2018

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“…Whereas flying 200 km or responding to an immune challenge can increase ROMs by approximately 0.27 mmol l −1 H 2 O 2 equivalents (Costantini et al, 2008b) and 0.74 mmol l −1 H 2 O 2 equivalents (van de Crommenacker et al, 2010), respectively, we detected an eatinginduced increase of over 1.25 mmol l −1 H 2 O 2 equivalents. This large increase is likely driven by increases in ROMs, rather than changes in ceruloplasmin or albumin levels, which can interfere with the kit's activity (Kilk et al, 2014; but see Colombini et al, 2016), for two reasons. First, increases in albumin concentration inhibit ROM activity (Kilk et al, 2014); while we quantified circulating protein levels rather than albumin levels per se, we detected greater (not reduced) ROM levels despite increased circulating protein concentration.…”

Section: Discussionmentioning

confidence: 99%

Eating increases oxidative damage in a reptile

Butler

Lutz

Fokidis

et al. 2016

Journal of Experimental Biology

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While eating has substantial benefits in terms of both nutrient and energy acquisition, there are physiological costs associated with digesting and metabolizing a meal. Frequently, these costs have been documented in the context of energy expenditure while other physiological costs have been relatively unexplored. Here, we tested whether the seemingly innocuous act of eating affects either systemic pro-oxidant (reactive oxygen metabolite, ROM) levels or antioxidant capacity of corn snakes (Pantherophis guttatus) by collecting plasma during absorptive ( peak increase in metabolic rate due to digestion of a meal) and non-absorptive (baseline) states. When individuals were digesting a meal, there was a minimal increase in antioxidant capacity relative to baseline (4%), but a substantial increase in ROMs (nearly 155%), even when controlling for circulating nutrient levels. We report an oxidative cost of eating that is much greater than that due to long distance flight or mounting an immune response in other taxa. This result demonstrates the importance of investigating non-energetic costs associated with meal processing, and it begs future work to identify the mechanism(s) driving this increase in ROM levels. Because energetic costs associated with eating are taxonomically widespread, identifying the taxonomic breadth of eating-induced ROM increases may provide insights into the interplay between oxidative damage and life history theory.

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“…dROMs are hydroperoxydes, meaning reactive oxygen metabolites that allow to directly evaluate oxidative stress levels [61,62]. SOD and GPX are antioxidant enzymes whose activity measurement indirectly allows to quantifying the response to oxidative stress [63,64].…”

Section: Discussionmentioning

confidence: 99%

Inflammatory and oxidative status in European captive black rhinoceroses: a link with Iron Overload Disorder?

Pouillevet

Soetart

Boucher

et al. 2020

Preprint

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17Iron Overload Disorder (IOD) is a syndrome developed by captive browsing 18 rhinoceroses like black rhinoceroses (Diceros bicornis) in which hemosiderosis settles in vital 19 organs while free iron accumulates in the body, potentially predisposing to various secondary 20 diseases. Captive grazing species like white rhinoceroses (Ceratotherium simum) do not seem 21 to be affected. The pro-oxidant and pro-inflammatory properties of iron, associated with the 22 poor antioxidant capacities of black rhinoceroses, could enhance high levels of inflammation 23 and oxidative stress leading to rapid ageing and promoting diseases. In this prospective study, 24 15 black (BR) and 29 white rhinoceroses (WR) originating from 22 European zoos were blood-25 sampled and compared for their iron status (serum iron), liver/muscle biochemical parameters 26 (AST, GGT, cholesterol), inflammatory status (total proteins, protein electrophoresis) and 27 oxidative stress markers (SOD, GPX, dROMs). Results showed higher serum iron and liver 28 enzyme levels in black rhinoceroses (P<0.01), as well as higher GPX (P<0.05) and dROM 29 (P<0.01) levels. The albumin/globulin ratio was lower in black rhinoceroses (P<0.05) due to 30 higher  2 -globulin levels (P<0.001). The present study suggests a higher inflammatory and 31 oxidative profile in captive BR than in WR, possibly in relation to iron status. This could be 32 either a consequence or a cause of iron accumulation, potentially explaining rapid ageing and 33 various diseases. Further investigations are needed to assess the prognostic value of the 34 inflammatory and oxidative markers in captive black rhinoceroses, particularly for evaluating 35 the impact of reduced-iron and antioxidant-supplemented diets. 36

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Oxidative stress, d-ROMs test, and ceruloplasmin

Cited by 31 publications

References 29 publications

Effect of growth hormone replacement therapy on plasma diacron-reactive oxygen metabolites and endothelial function in Japanese patients: The GREAT clinical study

Effect of growth hormone replacement therapy on plasma diacron-reactive oxygen metabolites and endothelial function in Japanese patients: The GREAT clinical study

Eating increases oxidative damage in a reptile

Inflammatory and oxidative status in European captive black rhinoceroses: a link with Iron Overload Disorder?

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