Oxidative stress-dependent p66Shc phosphorylation in skin fibroblasts of children with mitochondrial disorders

Lebiedzińska, Magdalena; Karkucińska-Więckowska, Agnieszka; Giorgi, Carlotta; Karczmarewicz, Elżbieta; Pronicka, Ewa; Pinton, Paolo; Duszyński, Jerzy; Pronicki, Maciej; Wiêckowski, Mariusz R.

doi:10.1016/j.bbabio.2010.04.186

Cited by 10 publications

(15 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Numerous studies suggest positive correlation between ΔΨ m and ROS production . However, in cases of mitochondrial disorders associated with dysfunction of the ETC, lower ΔΨ m with reduced respiration can be observed with simultaneous increase in ROS production . We show that despite reduced maximal respiration capacity in response to stress (FCCP), we observed increased basal ROS production in osteocytes from young and aged GHRKO mice; these data could be interpreted in two ways: 1) increased ROS in GHRKO osteocytes leads to a secondary increase in stress defense mechanisms, which protect the cells against further damage, or 2) increased ROS increases the sensitivity of GHRKO osteocytes to stress and, thereby, severely compromises their ability to resist further stress.…”

Section: Discussionmentioning

confidence: 48%

Mitochondrial Function Is Compromised in Cortical Bone Osteocytes of Long-Lived Growth Hormone Receptor Null Mice

Liu

Solesio

Schaffler

et al. 2018

Journal of Bone and Mineral Research

View full text Add to dashboard Cite

Despite increased longevity and resistance to multiple stressors, growth hormone receptor null (GHRKO) mice exhibit severe skeletal impairment. The role of GHR in maintaining osteocyte mitochondrial function is unknown. We found that GHR ablation was detrimental to osteocyte mitochondrial function. In vivo multiphoton microscopy revealed significant reductions of >10% in mitochondrial membrane potential (MMP) in GHRKO osteocytes and reduced mitochondrial volumetric density. Reductions in MMP were accompanied by reductions in glucose transporter-1 levels, steady state ATP, NADH redox index, oxygen consumption rate, and mitochondrial reserve capacity in GHRKO osteocytes. Glycolytic capacity did not differ between control and GHRKO males' osteocytes. However, osteocytes from aged female GHRKO mice exhibited reductions in glycolytic parameters, indicating impairments in glucose metabolism, which may be sex dependent. GHRKO osteocytes exhibited increased levels of cytoplasmic reactive oxygen species (ROS) (both basal and in response to high glucose), insulin-like growth factor-1 (IGF-1), and insulin. Mitochondrial ROS levels were increased and correlated with reduced glutathione in GHRKO osteocytes. Overall, the compromised osteocyte mitochondrial function and responses to metabolic insults strongly correlated with skeletal impairments, suggesting that despite increased life span of the GHRKO mice, skeletal health span is decreased. © 2018 American Society for Bone and Mineral Research.

show abstract

Section: Discussionmentioning

confidence: 48%

Mitochondrial Function Is Compromised in Cortical Bone Osteocytes of Long-Lived Growth Hormone Receptor Null Mice

Liu

Solesio

Schaffler

et al. 2018

Journal of Bone and Mineral Research

View full text Add to dashboard Cite

show abstract

“…Importantly, Cu-induced MMP dissipation was inhibited by NAC and vitamin E suggesting involvement of ROS in this response. Note that while a strong positive correlation between MMP and ROS generation has repeatedly been observed (Suski et al, 2012) increased ROS production at low MMP has also been reported (Zamzami et al, 1995;Nicholls and Ward, 2000;Murphy, 2009;Lebiedzinska et al, 2010).…”

Section: Effect Of Cu and Temperature On Membrane Potentialmentioning

confidence: 75%

Interactions of copper and thermal stress on mitochondrial bioenergetics in rainbow trout, Oncorhynchus mykiss

et al. 2014

View full text Add to dashboard Cite

“…Previous data indicates mitochondria with reduced respiratory chain activity and subsequent membrane potential are associated with increased ROS formation [35] though this seems to be the exception rather than the rule, as increased mitochondrial membrane potential is more often associated with increased ROS production [36]. Interestingly, micromolar concentrations of cannabinoids inhibit activity of respiratory chain complexes II, III, and IV [37].…”

Section: Discussionmentioning

confidence: 96%

Chronic Marijuana Smoking Does Not Negatively Impact Select Blood Oxidative Stress Biomarkers in Young, Physically Active Men and Women

Butawan¹,

Smith²

2018

Health

View full text Add to dashboard Cite

Background: The smoking of Cannabis sativa, the marijuana plant, is increasing in popularity among young adults, even those who may be engaged in regular exercise (i.e., athletes). Research has shown the plant to have antioxidant and analgesic properties, but the effects on oxidative stress are conflicting. The purpose of this study was to measure blood oxidative stress and cardio-metabolic parameters in physically active men and women who regularly smoke marijuana. Methods: A total of 43 marijuana smokers (23 ± 4 years) and 22 non-smokers (24 ± 7 years), who did not smoke tobacco products, participated in this study. Both smokers and non-smokers engaged in regularly exercise, totaling several hours per week (6.4 ± 4.0 and 6.8 ± 4.4, respectively). Smokers reported using marijuana frequently during the week (4.5 ± 2.3 sessions) for a minimum of three consecutive months prior to participating in the study. Blood samples were collected from participants following a 12-hour fast (all food, drink [except water] and smoking) and analyzed for malondialdehyde, advanced oxidation protein products, glucose, cholesterol, and triglycerides. Heart rate and blood pressure was also measured and recorded. Results: No differences of statistical significance were noted for any variable (p > 0.05), with very similar values noted between smokers and non-smokers. Conclusions: In a sample of young, physically active men and women, regular marijuana smoking is not associated with untoward effects on select biomarkers of oxidative stress and cardio-metabolic health. These findings do not suggest that marijuana smoking can be done without harm, as limitations of this study need to be considered.

show abstract

Oxidative stress-dependent p66Shc phosphorylation in skin fibroblasts of children with mitochondrial disorders

Cited by 10 publications

References 0 publications

Mitochondrial Function Is Compromised in Cortical Bone Osteocytes of Long-Lived Growth Hormone Receptor Null Mice

Mitochondrial Function Is Compromised in Cortical Bone Osteocytes of Long-Lived Growth Hormone Receptor Null Mice

Interactions of copper and thermal stress on mitochondrial bioenergetics in rainbow trout, Oncorhynchus mykiss

Chronic Marijuana Smoking Does Not Negatively Impact Select Blood Oxidative Stress Biomarkers in Young, Physically Active Men and Women

Contact Info

Product

Resources

About