Oxidative stress in an assisted reproductive techniques setting

Agarwal, Ashok; Said, Tamer M.; Bedaiwy, Mohamed A.; Banerjee, Jashoman; Álvarez, Juan G.

doi:10.1016/j.fertnstert.2006.02.088

Cited by 309 publications

(258 citation statements)

References 102 publications

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“…In contrast, DGC has a great potential in the isolation of mature, leukocyte-free spermatozoa, and MACS is a simple and fast sorting system for the separation of large numbers of cells. The leukocytes and morphologically abnormal spermatozoa can be isolated and excluded from sperm suspensions by means of MACS using paramagnetic microbeads [36]. Removal of abnormal spermatozoa decreases the oxidative stress among vital and healthy cells.…”

Section: Discussionmentioning

confidence: 99%

Clinical outcome of magnetic activated cell sorting of non-apoptotic spermatozoa before density gradient centrifugation for assisted reproduction

Dırıcan¹,

Özgün²,

Akarsu³

et al. 2008

J Assist Reprod Genet

127

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Purpose Magnetic activated cell sorting (MACS) eliminates apoptotic spermatozoa based on the presence of externalized phosphatidylserine residues. We evaluated the outcome of male fertility treatment when intracytoplasmic sperm injection (ICSI) into human oocytes was performed with non-apoptotic MACS-selected spermatozoa. Methods 196 couples were treated by ICSI following spermatozoa preparation by MACS (study group; 122 couples) or density gradient centrifugation (DGC) (control group; 74 couples). Fertilization, cleavage, pregnancy, and implantation rates were analyzed. Results The percentage of sperm with normal morphology after MACS selection was improved. Cleavage and pregnancy rates were higher, respectively, in the study group than in control. A slightly higher implantation rate was also observed in the study group. Conclusions MACS selection of human spermatozoa increased cleavage and pregnancy rates in oligoasthenozoospermic ART cases. This novel method for selecting nonapoptotic spermatozoa for ICSI is safe and reliable, and may improve the assisted reproduction outcome.

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Section: Discussionmentioning

confidence: 99%

Clinical outcome of magnetic activated cell sorting of non-apoptotic spermatozoa before density gradient centrifugation for assisted reproduction

Dırıcan¹,

Özgün²,

Akarsu³

et al. 2008

J Assist Reprod Genet

127

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“…Oxidative stress has been known as excessive production of ROS or imbalance between the production of ROS and antioxidant defense system (i.e., oxidants exceed antioxidant [7,22]). Under physiological conditions, generation of ROS occurs during various cellular metabolic reactions which is equilibrated by antioxidant defense systems of cells in order to neutralize the reactive intermediate [4,6]. In the in vivo condition, enzymatic and non-enzymatic antioxidants provide adequate protection for OS-induced pathological changes and maintain an optimal level of ROS, whereas in the in vitro setup, higher oxygen levels and lack of physiological defense mechanisms against ROS result in OS (Sajal [34,47]).…”

Section: Introductionmentioning

confidence: 99%

The effect of alpha lipoic acid on the developmental competence of mouse isolated preantral follicles

Talebi

Zavareh

Kashani

et al. 2012

J Assist Reprod Genet

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Purpose This study was designed to investigate the effect of alpha-lipoic acid (ALA) on reactive oxygen species (ROS) production, total antioxidant capacity (TAC) and developmental competence of cultured pre-antral follicles derived from mouse ovarian tissue. Methods Pre-antral follicles were isolated from immature mouse ovaries and were cultured in α-minimal essential medium supplemented with different concentrations (0, 50, 100, 250 and 500 uM) of ALA. Follicular growth, oocyte maturation and embryo development were evaluated. Separately, ROS and TAC were measured after 0, 24, 48, 72 and 96 h of culture with spectrofluorometery and ferric reducing/antioxidant power (FRAP) assay, respectively. Results In the presence of 100 uM ALA, developmental rates of follicles, oocytes and embryos were significantly higher than other groups (p<0.05). At 96 h after culture, a decrease in ROS and an increase in TAC were observed in ALA group compared to control group (p<0.05). Conclusion ALA (100 uM) improves the in vitro development of follicles. This effect may be mediated by decreasing ROS concentration and increasing follicular TAC level during the culture period.

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“…Numerous reports have indicated that significantly increased ROS levels may occur in response to repeated cycles of centrifugation involved in conventional sperm preparation techniques used for ARTs [179]. Spermatozoa selected for ART often face OS and a high risk for DNA damage.…”

Section: Carnitinementioning

confidence: 99%

“…The density gradient technique is able to separate leukocytes and immature or damaged spermatozoa from normal spermatozoa, which may be subsequently used in ARTs [77,179,180].…”

Section: Carnitinementioning

confidence: 99%

See 1 more Smart Citation

Physiological and Pathological Roles of Free Radicals in Male Reproduction

Tvrdá¹,

Massányi²,

Lukáč³

2018

Spermatozoa - Facts and Perspectives

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Oxidative stress (OS) is a condition caused by an imbalance between reactive oxygen species (ROS) overgeneration and decreased antioxidant defense mechanisms in the cell. OS has become a prominent factor in male reproductive dysfunction as ROS cause damage to sperm DNA, lipids and proteins, alterations to critical sperm structures and signaling pathways, leading to a decreased sperm activity and fertilizing capacity. At the same time, small amounts of ROS play vital roles in events leading to sperm maturation and acquisition of functional activity, which is why a proper oxidative balance is of paramount importance for a proper male fertility. Understanding the physiological and pathological roles of ROS in male reproduction has become an essential pillar of modern andrology; however, numerous questions related to the controversial behavior of ROS in male reproductive cells and tissues still remain unanswered. This chapter aims to summarize current evidence available on the relationships between free radicals, antioxidants and male reproduction and to trigger more scientific interest, particularly with respect to the design of efficient strategies to diagnose or treat male sub-or infertility associated with OS.

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Oxidative stress in an assisted reproductive techniques setting

Cited by 309 publications

References 102 publications

Clinical outcome of magnetic activated cell sorting of non-apoptotic spermatozoa before density gradient centrifugation for assisted reproduction

Clinical outcome of magnetic activated cell sorting of non-apoptotic spermatozoa before density gradient centrifugation for assisted reproduction

The effect of alpha lipoic acid on the developmental competence of mouse isolated preantral follicles

Physiological and Pathological Roles of Free Radicals in Male Reproduction

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