Oxidative Stress in Caffeine Action on the Proliferation and Death of Human Breast Cancer Cells MCF-7 and MDA-MB-231

Machado, Kaliana Larissa; Marinello, Poliana Camila; Silva, Thamara Nishida Xavier; Silva, Cássio Fernando Nunes; Luiz, Rodrigo Cabral; Cecchini, Rúbens; Cecchini, Alessandra Lourenço

doi:10.1080/01635581.2020.1795693

Cited by 16 publications

(8 citation statements)

References 47 publications

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“…Caffeine can potentially increase the antioxidant defenses of breast tumor cells. The same was observed in MCF-7 and MDA-MB-231 cells treated with caffeine [38].…”

Section: The Activity Of the Enzyme Superoxide Dismutase (Sod) Cellul...supporting

confidence: 78%

“…Caffeine can modulate both the innate and adaptive immune response [37]. It affects the cell cycle [38] and acts on protein kinases that play important roles in repairing DNA damage, which induces strain of the cell cycle in phase G1 and signaling apoptosis [39]. It also can increase antioxidant defenses [38], and chronic intake improves oxidative stress [40].…”

Section: Introductionmentioning

confidence: 99%

“…It affects the cell cycle [38] and acts on protein kinases that play important roles in repairing DNA damage, which induces strain of the cell cycle in phase G1 and signaling apoptosis [39]. It also can increase antioxidant defenses [38], and chronic intake improves oxidative stress [40]. Strategies to eliminate tumor cells include modulation of oxidative metabolism and strengthening of antitumor effects of drugs associated with a modified release system [25] [41].…”

Section: Introductionmentioning

confidence: 99%

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Modified Caffeine Release System and Its Immunomodulatory Effects on Breast Tumor Cells and Blood Phagocytes

Soares¹,

Torres²,

Dalcin³

et al. 2023

ABC

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Caffeine is an alkaloid present in a wide variety of plants. Currently the most consumed psychostimulant worldwide, its consumption is associated with several health benefits, including modulation of the innate and adaptive immune response, reduction of oxidative cellular stress, and decreased incidence of some cancers, including breast cancer. Breast cancer is the most common cause of cancer among women and the second leading cause of cancer death in women worldwide. The interaction between biomaterials and drugs has enabled a great advance in science for developing controlled drug delivery systems and has been used to treat numerous pathologies. This work aimed to evaluate the immunomodulatory effects of caffeine associated or not with polyethylene glycol adsorbed in microemulsion (MLP) on MCF-7 cells, phagocytic cells (MN), and coculture. For biological assays, ATCC (American Type Culture Collection, USA) cell lines of breast adenocarcinoma (MCF-7) and phagocytes (MN) obtained from voluntary donors were used. The cells (MN and MCF-7) and coculture were treated with caffeine and MLP and incubated for rheological characterization analyses: flow curve and viscosity, oxidative stress: superoxide anion assay ( 2 O − ), and activity of the enzyme superoxide dismutase (Cu-Zn-SOD). Caffeine and MLP increased viscosity and blood and MCF-7 cells and affected the immunomodulation of oxidative stress metabolism of MN and MCF-7 cells treated with caffeine and associated caffeine to the MLP. These data suggest that caffeine is associated or not with MLP-induced immunomodulatory effects on MN phagocytes and

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“…Caffeine can potentially increase the antioxidant defenses of breast tumor cells. The same was observed in MCF-7 and MDA-MB-231 cells treated with caffeine [38].…”

Section: The Activity Of the Enzyme Superoxide Dismutase (Sod) Cellul...supporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Modified Caffeine Release System and Its Immunomodulatory Effects on Breast Tumor Cells and Blood Phagocytes

Soares¹,

Torres²,

Dalcin³

et al. 2023

ABC

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“…The administration of CAF in biological systems has been related to cell death [ 9 , 83 , 84 , 85 ] cell proliferation [ 86 ], and the prevention of apoptotic processes [ 11 , 87 ]. It seems ambiguous, but the effect of CAF on cell responses depends on its concentration, the type of cell, and the relevant experimental conditions [ 88 ].…”

Section: Resultsmentioning

confidence: 99%

Caffeine Release from Magneto-Responsive Hydrogels Controlled by External Magnetic Field and Calcium Ions and Its Effect on the Viability of Neuronal Cells

et al. 2023

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Caffeine (CAF) is a psychostimulant present in many beverages and with rapid bioabsorption. For this reason, matrices that effectuate the sustained release of a low amount of CAF would help reduce the intake frequency and side effects caused by high doses of this stimulant. Thus, in this study, CAF was loaded into magnetic gelatin/alginate (Gel/Alg/MNP) hydrogels at 18.5 mg/ghydrogel. The in vitro release of CAF was evaluated in the absence and presence of an external magnetic field (EMF) and Ca2+. In all cases, the presence of Ca2+ (0.002 M) retarded the release of CAF due to favorable interactions between them. Remarkably, the release of CAF from Gel/Alg/MNP in PBS/CaCl2 (0.002 M) at 37 °C under an EMF was more sustained due to synergic effects. In PBS/CaCl2 (0.002 M) and at 37 °C, the amounts of CAF released after 45 min from Gel/Alg and Gel/Alg/MNP/EMF were 8.3 ± 0.2 mg/ghydrogel and 6.1 ± 0.8 mg/ghydrogel, respectively. The concentration of CAF released from Gel/Alg and Gel/Alg/MNP hydrogels amounted to ~0.35 mM, thereby promoting an increase in cell viability for 48 h. Gel/Alg and Gel/Alg/MNP hydrogels can be applied as reservoirs to release CAF at suitable concentrations, thus forestalling possible side effects and improving the viability of SH-SY5Y cells.

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“…Buranrat et al reported that the combination of DOX and SIM increased cytochrome c protein expression and caspase-3 activity compared to each drug alone, suggesting that SIM sensitizes MCF-7 breast tumor cells, potentiating the action of DOX [ 47 ]. Furthermore, Machado and coworkers suggested that MCF-7 cells are more resistant to oxidative damage caused by ROS compared to MDA-MB-231 cells, thus suffering less apoptosis [ 48 ]. This fact could explain the results obtained herein, in which IC50 values for MCF-7 were higher than MDA-MB-231 cells, especially after treatment with liposomal formulations.…”

Section: Discussionmentioning

confidence: 99%

Co-Encapsulation of Simvastatin and Doxorubicin into pH-Sensitive Liposomes Enhances Antitumoral Activity in Breast Cancer Cell Lines

et al. 2023

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Doxorubicin (DOX) is a potent chemotherapeutic drug used as the first line in breast cancer treatment; however, cardiotoxicity is the main drawback of the therapy. Preclinical studies evidenced that the association of simvastatin (SIM) with DOX leads to a better prognosis with reduced side effects and deaths. In this work, a novel pH-sensitive liposomal formulation capable of co-encapsulating DOX and SIM at different molar ratios was investigated for its potential in breast tumor treatment. Studies on physicochemical characterization of the liposomal formulations were carried out. The cytotoxic effects of DOX, SIM, and their combinations at different molar ratios (1:1; 1:2 and 2:1), free or co-encapsulated into pH-sensitive liposomes, were evaluated against three human breast cancer cell lines (MDA-MB-231, MCF-7, and SK-BR-3). Experimental protocols included cell viability, combination index, nuclear morphological changes, and migration capacity. The formulations showed a mean diameter of less than 200 nm, with a polydispersity index lower than 0.3. The encapsulation content was ~100% and ~70% for DOX and SIM, respectively. A more pronounced inhibitory effect on breast cancer cell lines was observed at a DOX:SIM molar ratio of 2:1 in both free and encapsulated drugs. Furthermore, the 2:1 ratio showed synergistic combination rates for all concentrations of cell inhibition analyzed (50, 75, and 90%). The results demonstrated the promising potential of the co-encapsulated liposome for breast tumor treatment.

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Oxidative Stress in Caffeine Action on the Proliferation and Death of Human Breast Cancer Cells MCF-7 and MDA-MB-231

Cited by 16 publications

References 47 publications

Modified Caffeine Release System and Its Immunomodulatory Effects on Breast Tumor Cells and Blood Phagocytes

Modified Caffeine Release System and Its Immunomodulatory Effects on Breast Tumor Cells and Blood Phagocytes

Caffeine Release from Magneto-Responsive Hydrogels Controlled by External Magnetic Field and Calcium Ions and Its Effect on the Viability of Neuronal Cells

Co-Encapsulation of Simvastatin and Doxorubicin into pH-Sensitive Liposomes Enhances Antitumoral Activity in Breast Cancer Cell Lines

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