Oxidative Stress in Human Thyroid Gland Under Iodine Deficiency Nodular Goiter: From Harmlessness to Hazard Depending on Copper and Iodine Subcellular Distribution

Falfushynska, Halina; Gnatyshyna, Lesya; Shul’gai, A. G.; Shidlovski, V.; Stoliar, Оksana

doi:10.11603/ijmmr.2413-6077.2015.1.2822

Cited by 3 publications

(4 citation statements)

References 28 publications

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“…[3] and are accu mulated under an imbalance between their production and elimination by protective mechanisms, referred to as antioxidants. ROS are overproduced a long time, then, may cause DNA, protein, and/or lipid damage [7]. In our case the ovary cancer is also said to be under oxidative stress (IOS less than zero) associates with the most prominent upraise of oxidative lesions (triple of them, TBARS, protein carbonyls and oxyradicals).…”

Section: Resultsmentioning

confidence: 81%

“…One of the most common mechanisms of diseases development and aggressiveness of pathology which is on the focus of biomedical research has been linked to overexpression of free radicals and oxidative stress initiation [3,4,5,6,7]. The damage that reactive oxygen species (ROS) can cause to the cell not only depends on their internal concentration but also on the equilibrium between the ROS and the endogenous antioxidants normally protect cells against oxidative stress [4,6].…”

Section: Introductionmentioning

confidence: 99%

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Detection of Oxidative Stress, Apoptosis and Molecular Lesions in Human Ovarian Cancer Cells

Falfushynska¹

2016

IJMMR

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Background. Ovarian cancer has the highest mortality rate of gynaecological cancers. This is partly due to the lack of effective screening markers. Indices of oxidative stress are well-recognized prognostic criteria for tumorous transformation of tissue, but their value depends on the type of tumor and the stage of its development. Objective. The aim of this study is to clarify the relationship between antioxidant/pro-oxidant ratio and the signs of molecular lesions and apoptosis rate in blood of ovarian cancer patients and non-cancer ones. Results. The ovarian cancer group is marked by antioxidant/prooxidant balance shifting to oxidative damage in blood as the consequence of overexpression of oxyradicals (by 300%). Higher level of glutathione (by 366%), lower level of metallothioneins (by 65%) as well as higher level of lipid peroxidation (by 174%) and protein carbonyls (by 186%) in blood of ovarian cancer patients compared to the normal ovarian group have been observed. The signs of cytotoxicity are determined in blood of ovarian cancer patients: an increased (compared to control) level of DNA fragmentation (by 160%), choline esterase (up to twice), higher rate of both caspase dependent and caspase independent lysosomal mediated apoptosis. Conclusions. Cathepsin D activity both total and free, choline esterase activity, TBA-reactive substance and protein carbonyls level in blood could be used as the predictive markers of worse prognosis and the signs of human ovarian cancer. KEY WORDS: ovarian cancer, oxidative stress, apoptosis, caspase-3, cathepsin D, choline esterase, metallothionein.

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Section: Resultsmentioning

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Detection of Oxidative Stress, Apoptosis and Molecular Lesions in Human Ovarian Cancer Cells

Falfushynska¹

2016

IJMMR

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“…It is generally known that iodine deficiency develop in pathologically affected immune system. We suspected that hyperplastic thyroid epithelium under iodine deficiency sacrificed its ability for iodine organification and therefore increased of level of inorganic iodine [26].…”

Section: Discussionmentioning

confidence: 99%

“…These changes lead to gradual hyperplasia of the epithelium of thyroid cells. Which lose the ability to organize all iodine in the gland and bring to excessive accumulation of inorganic iodine in organs and tissues, blood serum, hair, urine [26]. As a result, there is a shortage of "building material" for thyroid hormones and the development of hypothyroxinemia.…”

Section: Discussionmentioning

confidence: 99%

Oxidative Stress Manifestation for Community-Acqiured Pneumonia in Children Living in Endemic Area of Iodine Deficiency

Pavlyshyn¹,

Kibar²,

Slyva³

2018

Acta Medica

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Objective: We aimed to evaluate the state of oxidative stress, level of molecular damage in relationship with the subcellular distribution of iodine in patients with community-acquired pneumonia from the iodine deficiency region. Materials and Methods: Our study involved 70 children, 4-14 years old, with CAP (moderate severity in 35 children (Group 1); severe - in 35 (Group 2)) and 35 healthy children of the control group (Group 3). The state of organificated/inorganic, antioxidant defense system, stress-dependent systems, apoptosis and the level of molecular damage were assessed in serum samples. Results: Lower concentration of organificated iodine (12% and 59%, respectively) and high concentration of inorganic iodine (21% and 48%, respectively) were found in Groups 1 and 2. Patients of Group 2 had an inverse relationship between the levels of iodine distribution for the organic and inorganic iodine (r = - 0.645, p<0.05). In patients of Group 1, this relationship tends to decrease. The oxidative stress indicators correlated with disease severity. ROS generation indices in patients of Group 1 is 28.1 (24.2; 32.1) RFU/mg; (p < 0.05); Group 2 – 40.2 (34.2; 42.5) RFU/mg (p < 0.05); Caspase-3 is 25.77 (21.45–32.16) pmol/mg Group 1 and 39.42 (32.41–44.21) pmol/mg Group 2, (respectively, p < 0.05). We found that children with a severe pneumonia have a prooxidant activation, manifested by a significant increase of oxidation-modified proteins [2.21 (1.88–2.53) nmol/mg)] and DNA fragmentation [13.42 (10.3–15.46) %], (p < 0.05). The extent of DNA damage correlates with the extent of apoptosis. Conclusions: Increase in the concentration of inorganic iodine with a simultaneous decrease in the level of organic iodine in the blood, which leads to a more severe course of pneumonia. Fluctuations in the indices of oxidative stress, the level of molecular damage and the intensity of apoptosis in children with pneumonia correlate with the severity of the disease.

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Iodine Status and Patterns of Oxidative Metabolism Disorders in Children With Community-Acqiured Pneumonia From the Iodine Deficiency Region

Павлишин¹,

Kibar²

2018

Neonatol. hìr. perinat. med.

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Oxidative Stress in Human Thyroid Gland Under Iodine Deficiency Nodular Goiter: From Harmlessness to Hazard Depending on Copper and Iodine Subcellular Distribution

Cited by 3 publications

References 28 publications

Detection of Oxidative Stress, Apoptosis and Molecular Lesions in Human Ovarian Cancer Cells

Detection of Oxidative Stress, Apoptosis and Molecular Lesions in Human Ovarian Cancer Cells

Oxidative Stress Manifestation for Community-Acqiured Pneumonia in Children Living in Endemic Area of Iodine Deficiency

Iodine Status and Patterns of Oxidative Metabolism Disorders in Children With Community-Acqiured Pneumonia From the Iodine Deficiency Region

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