Oxidative stress in liver cell culture from mullet, Liza klunzingeri, induced by short-term exposure to benzo[a]pyrene and nonylphenol

Salamat, Negin; Derakhshesh, Negin

doi:10.1007/s10695-020-00783-y

Cited by 12 publications

(10 citation statements)

References 59 publications

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“…In the present study, SOD activities were signi cantly increased with all of the BPF concentrations when compared to the control group, presumably in response to enhanced cellular superoxide anion radicals. In accordance with the present study, in vitro studies using sh hepatocytes have also reported increased SOD activities after exposure to different types of environmental chemicals, such as BPA [35], per uorinated organic compounds [29], di(2ethylhexyl) phthalate [36], and benzo[a]pyrene and nonylphenol [37]. On the other hand, no signi cant changes were seen in the SOD activity of juvenile common carp liver (Cyprinus carpio) samples after long-term (60 days) waterborne exposure to BPF [38].…”

Section: Discussionsupporting

confidence: 91%

“…Similar to these ndings, Maćczak et al found decreased levels of CAT in human erythrocytes treated with BPF for different periods of time (4 h and 24 h) [15]. Decreased CAT activity was also found in juvenile common carp [37], and the larvae of zebra sh after waterborne exposure to BPF [7]. Ullah et al, also reported that CAT activity was inhibited in the testicular tissues of rats exposed to BPF via drinking water [5].…”

Section: Discussionmentioning

confidence: 56%

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In vitro effects of bisphenol F on antioxidant system indicators in the isolated hepatocytes of rainbow trout (Oncorhyncus mykiss)

Aykut

Kaptaner

2021

Mol Biol Rep

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Bisphenol F (BPF) has been used frequently in the plastics industry and the production of daily consumer products as an alternative to bisphenol A (BPA). It was aimed herein to determine the cytotoxic effects of BPF on hepatocytes isolated from the liver of rainbow trout (Oncorhyncus mykiss) using lactate dehydrogenase (LDH) assay and antioxidant defence system indicators. The cultured hepatocytes were exposed to seven concentrations (0, 15.63, 31.25, 62.50, 125, 250, and 500 µM) of BPF for 24 h. According to the LDH assay, the percentage of cytotoxicity was increased dose dependently in the cells.The malondialdehyde content, which is indicative of lipid peroxidation, was increased signi cantly at BPF concentrations between 15.63 and 250 µM, whereas it remained unchanged with a concentration of 500 µM. The activities of superoxide dismutase were increased, while those of catalase were decreased with all of the BPF concentrations. Elevated levels of reduced glutathione content were determined with BPF concentrations between 15.63 and 250 µM, but decreased signi cantly with a concentration of 500 µM.Signi cant increases in the activities of the glutathione peroxidase were found in hepatocytes treated with BPF at concentrations of 31.25 to 500 µM. GST activity was only signi cantly increased with a BPF concentration of 250 µM. The results showed that the toxic mechanism of BPF was mainly based on cell membrane damage and oxidative stress, which have an in uence on antioxidant defences. Therefore, BPF was reconsidered as a safe alternative instead of BPA in the manufacturing of industrial or daily products.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 56%

In vitro effects of bisphenol F on antioxidant system indicators in the isolated hepatocytes of rainbow trout (Oncorhyncus mykiss)

Aykut

Kaptaner

2021

Mol Biol Rep

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“…This is particularly worrisome as their mechanisms of action may coincide with that of APAP. Among these chemicals are the polycyclic aromatic hydrocarbons (PAHs), in particular benzo[a]pyrene (B[a]P), known for their capacity to induce oxidative stress [30][31][32]. Benzo[a]pyrene is considered a high-priority substance of concern for human exposure (8th/275), according to the Agency for Toxic Substances and Disease Registry [33].…”

Section: Introductionmentioning

confidence: 99%

“…Due to its lipophilicity, B[a]P is readily absorbed through biological membranes and undergoes bioactivation to reactive metabolites mediated by enzymes from the Cytochrome P450 (CYP) superfamily, including cytochrome P450 1A1 (CYP1A1), cytochrome P450 1A2 (CYP1A2), and cytochrome P450 1B1 (CYP1B1), resulting in the production of ROS and metabolites such as phenol forms, epoxides, dihydrodiols, dihydrodiol epoxides, and anti-7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydro-B[a]P [30,32,35,38,44,45]. It has been demonstrated that the production of B[a]P-7,8-epoxide, mediated by CYP1A1, and its subsequent transformation to B[a]P-trans-7,8-dihydrodiol (B[a]P-7,8-DHD) in the presence of epoxide hydrolase represent dangerous reactions, as B[a]P-7,8-DHD is converted to the carcinogenic metabolite 7β,8α-dihydroxy-9α,10α-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE), which creates DNA adducts, causing mutations and malignant transformations [35,46].…”

Section: Introductionmentioning

confidence: 99%

“…The cellular antioxidant system, including key enzymes such as superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), and non-enzymatic components like glutathione, plays a crucial role in eliminating or regulating the elevated levels of ROS produced in the body [30]. Despite this, some studies have demonstrated disturbances in antioxidant responses in the liver, lungs, kidneys, stomach, and brain induced by acute B[a]P treatment in a murine model [30,32].…”

Section: Introductionmentioning

confidence: 99%

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Exposure to Benzo(a)pyrene Enhances Acetaminophen-Induced Liver Injury in Mice at Non-Hepatotoxic Doses

Montero-Pérez,

Olivero-Verbel

2024

Sci. Pharm.

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Acetaminophen (APAP) is a widely used analgesic, especially for children. Its primary mechanism involves inhibiting cyclooxygenase enzymes and activating the endocannabinoid and TRPV1 systems. Though its toxicity is low, it can harm the liver in a dose-dependent manner. Low APAP doses can also increase pollutant-induced liver damage. Little is known about interactions between APAP and benzo[a]pyrene (B[a]P). This study aimed to assess if co-exposure to non-hepatotoxic doses of B[a]P and APAP causes liver injury in mice, exploring the underlying mechanisms. Female ICR mice received 50 mg/kg B[a]P or a vehicle for three days, followed by 200 mg/kg APAP or a vehicle. Liver injury was assessed through histopathological examination, serum transaminase activity, and gene expression analysis. In the B[a]P/APAP group, several histology changes were observed, including ballooning injury, steatosis, necrosis, inflammation, and apoptosis. Transaminase levels correlated with histopathological scores, and there was an increase in hepatic cytochrome P450 family 1 subfamily a member 1 (Cyp1a1) mRNA levels and a decrease in aryl hydrocarbon receptor (Ahr), cytochrome P450 family 2 subfamily e polypeptide 1 (Cyp2e1), superoxide dismutase 1 (Sod1), peroxisome proliferator activated receptor gamma (Ppar-γ), and caspase 3 (Casp3). This suggests that prior exposure to B[a]P makes mice more susceptible to APAP-induced liver injury, involving changes in gene expression related to metabolism, redox balance, and cell proliferation. Therefore, using therapeutic APAP doses after exposure to B[a]P could lead to liver injury.

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Biomarker responses and histological damage in the gill, liver, and gonad of Cyprinus carpio with benzo(a)pyrene exposure

Sha

Cai

Liu

et al. 2021

Environ Sci Pollut Res

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Oxidative stress in liver cell culture from mullet, Liza klunzingeri, induced by short-term exposure to benzo[a]pyrene and nonylphenol

Cited by 12 publications

References 59 publications

In vitro effects of bisphenol F on antioxidant system indicators in the isolated hepatocytes of rainbow trout (Oncorhyncus mykiss)

In vitro effects of bisphenol F on antioxidant system indicators in the isolated hepatocytes of rainbow trout (Oncorhyncus mykiss)

Exposure to Benzo(a)pyrene Enhances Acetaminophen-Induced Liver Injury in Mice at Non-Hepatotoxic Doses

Biomarker responses and histological damage in the gill, liver, and gonad of Cyprinus carpio with benzo(a)pyrene exposure

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