Oxidative stress in patients with Graves' ophthalmopathy: relationship between oxidative DNA damage and clinical evolution

Tsai, Chieh Chih; Cheng, Ching‐Yu; Liu, C. Y.; Kao, Sung‐Shuo; Kau, Hui-Chuan; Hsu, Wen-Ming; Wei, Yau‐Huei

doi:10.1038/eye.2008.310

Cited by 62 publications

(58 citation statements)

References 40 publications

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“…Therefore, the oxidative DNA damage in cultured orbital fibroblasts is more reliable and specific than that in fibroadipose tissues, and the increased oxidative DNA damage in cultured orbital fibroblasts is also in accordance with our previous findings in urinary levels of 8-OHdG. 20 Oxidative DNA damage and lipid peroxidation were increased in GO orbital fibroblasts as compared with normal fibroblasts in this study implies that oxidative stress in orbital fibroblasts may have an important role in the pathogenesis of GO. This distinct phenotype exhibited by orbital fibroblasts may underlie the susceptibility of the orbit to manifestations of GO.…”

Section: Discussionsupporting

confidence: 80%

“…In our previous study, we showed that the urinary 8-hydroxy 2 0 -deoxyguanosine (8-OHdG) level, an important biomarker of oxidative DNA damage, is increased in patients with GO and is correlated with the clinical evolution of GO, especially the disease activity. 20 Furthermore, this biomarker of oxidative DNA damage could be reduced after treatment with systemic steroid in patients with active GO. 21 In this investigation, we determined the oxidative DNA damage in orbital fibroadipose tissues and cultured orbital fibroblasts from patients with GO and compared them with the controls.…”

Section: Introductionmentioning

confidence: 99%

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Increased oxidative DNA damage, lipid peroxidation, and reactive oxygen species in cultured orbital fibroblasts from patients with Graves’ ophthalmopathy: evidence that oxidative stress has a role in this disorder

Tsai

Wu²,

Cheng

et al. 2010

Eye

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Section: Discussionsupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Increased oxidative DNA damage, lipid peroxidation, and reactive oxygen species in cultured orbital fibroblasts from patients with Graves’ ophthalmopathy: evidence that oxidative stress has a role in this disorder

Tsai

Wu²,

Cheng

et al. 2010

Eye

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“…Oxidative stress appears to be involved in the development of several diseases such as endocrine and inflammatory diseases [Tsai et al 2009]. Moreover, several studies have reported that the oxidative stress was associated with autoimmune thyroid diseases such as GD and HT Aslan et al 2011;Žarković, 2012].…”

Section: Discussionmentioning

confidence: 99%

“…Oxidative stress arises when the production of ROS exceeds cellular ability to remove them and repair cellular damage, and ultimately leads to the widespread oxidative damage to macromolecules, including lipid, proteins and DNA [Tsai et al 2009, Marcocci et al 2012. Oxidative stress appears to be involved in the development of several diseases such as endocrine and inflammatory diseases [Tsai et al 2009].…”

Section: Discussionmentioning

confidence: 99%

Lipid peroxidation, proteins modifications, anti-oxidant enzymes activities and selenium deficiency in the plasma of hashitoxicosis patients

Mseddi

Mansour

Mnif

et al. 2015

Therapeutic Advances in Endocrinology

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Objectives: The aim of this study was to explore the oxidative stress profile in hashitoxicosis (HTX) and to compare it with that of healthy subjects. Patients and methods: Spectrophotometric methods were used to evaluate the oxidative stress markers. The selenium level was investigated by atomic absorption. Results: High levels of thiobarbituric acid reactive species (TBARS) and conjugated dienes were found in HTX patients (p = 0.034 and p = 0.043, respectively) compared with healthy controls. For antioxidant enzymes, superoxide dismutase (SOD) and catalase activities increased, whereas that of glutathione peroxidase (GPx) decreased (p = 0.000, p = 0.014, p = 0.000, respectively) compared with controls. A reduction in the level of selenium (p = 0.029) and thiol groups (p = 0.008) were shown in patients; however, levels of carbonyl group and malondialdehyde (MDA) protein adducts decreased (p = 0.000) compared with controls. Positive correlation was shown between levels of free thyroxine (FT4) and TBARS (r = 0.711, p = 0.048) and between FT4 level and SOD activity (r = 0.713, p = 0.047). Conversely, GPx activity presented a negative correlation with FT4 and free triiodothyronine (FT3) levels (r = -0.934, p = 0.001; r = -0.993, p = 0.000, respectively). In addition, GPx activity showed positive correlation with selenium level (r = 0.981, p = 0.019) and the FT3 level correlated negatively with the level of thiol groups (r = -0.892, p = 0.017). Conclusions: This study shows the presence of an oxidative stress and selenium deficiency in HTX patients and suggests that the hyperthyroid state is strongly implicated in the establishment of this disturbed oxidative profile.

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“…There is growing evidence that oxidative stress plays an important role in the pathogenesis of GO (Bartalena et al 2003, Bednarek et al 2005, Tsai et al 2009). Superoxide radicals stimulate OFs to proliferate and to produce glycosaminoglycan (Burch et al 1997), and H 2 O 2 is known to induce the expression of HLA-DR and heat shock protein 72 (Heufelder et al 1992).…”

Section: Discussionmentioning

confidence: 99%

Cigarette smoke extract-induced adipogenesis in Graves' orbital fibroblasts is inhibited by quercetin via reduction in oxidative stress

Yoon¹,

Lee²,

Chae³

et al. 2012

Journal of Endocrinology

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Cigarette smoking is known to aggravate Graves' orbitopathy (GO) severity by enhancing adipogenesis. We investigated the effect of quercetin, an antioxidant, on adipocyte differentiation induced by cigarette smoke extract (CSE) in primary cultured orbital fibroblasts (OFs) from GO patients. Freshly prepared CSE was added to the cells and H 2 O 2 was used as a positive control. Intracellular reactive oxygen species (ROS) generation and adipogenesis were measured. The expressions of proteins peroxisome proliferator-activated receptor (PPAR) g, CCAAT-enhancer-binding proteins (C/EBP) a and b, and heme oxygenase-1 (HO-1), an antioxidant enzyme, were examined during adipogenic differentiation. In result, CSE and H 2 O 2 dose-dependently stimulated intracellular ROS production in normal and Graves' OFs. The effect of 2% CSE was similar to that of 10 mM H 2 O 2 ; both concentrations were noncytotoxic and were used throughout the experiment. Quercetin pretreatment reduced the ROS generation stimulated by either CSE or H 2 O 2 in preadipocyte OFs. CSE and H 2 O 2 stimulated adipocyte differentiation in cultured OFs. The addition of quercetin (50 or 100 mM) suppressed adipogenesis. Quercetin also suppressed ROS generation in differentiating OFs during adipogenesis stimulated by CSE and H 2 O 2 . Additionally, the expressions of PPARg, C/EBPa, and C/EBPb proteins were reduced in the quercetin-treated OFs. Quercetin also reduced the CSE-and H 2 O 2 -induced upregulation of ROS and HO-1 protein in differentiated OFs and preadipocyte OFs. As shown in this study, quercetin inhibited adipogenesis by reducing ROS in vitro, supporting the use of quercetin in the treatment of GO. IntroductionOxidative stress is implicated in the pathogenesis of Graves' orbitopathy (GO), and cigarette smoking is known to be a major environmental factor that affects GO. Cigarette smoking has been shown to influence the incidence, severity, and responses to treatment of GO, and appears to do so in a dose-dependent and temporal manner. Reportedly, smokers with Graves' disease are approximately five times more likely to develop GO than Journal of Endocrinology Research J S YOON, H J LEE and othersTreatment of GO by quercetin, an antioxidant 216:2 145-156

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Oxidative stress in patients with Graves' ophthalmopathy: relationship between oxidative DNA damage and clinical evolution

Cited by 62 publications

References 40 publications

Increased oxidative DNA damage, lipid peroxidation, and reactive oxygen species in cultured orbital fibroblasts from patients with Graves’ ophthalmopathy: evidence that oxidative stress has a role in this disorder

Increased oxidative DNA damage, lipid peroxidation, and reactive oxygen species in cultured orbital fibroblasts from patients with Graves’ ophthalmopathy: evidence that oxidative stress has a role in this disorder

Lipid peroxidation, proteins modifications, anti-oxidant enzymes activities and selenium deficiency in the plasma of hashitoxicosis patients

Cigarette smoke extract-induced adipogenesis in Graves' orbital fibroblasts is inhibited by quercetin via reduction in oxidative stress

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