Oxidative Stress in the Muscles of the Fish Nile Tilapia Caused by Zinc Oxide Nanoparticles and Its Modulation by Vitamins C and E

Abdelazim, Aaser M.; Saadeldin, Islam M.; Swelum, Ayman A.; Afifi, Mohamed; Alkaladi, Ali

doi:10.1155/2018/6926712

Cited by 74 publications

(31 citation statements)

References 60 publications

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“…None of the diets enriched with chemically synthesized ZnO nanopowder could augment the GR activity in the liver and gills. Our results coincide with results already reported, which proved that ZnO NPs inhibited SOD, CAT and GPx activities in juvenile carp (Hao and Chen 2012), and in Nile tilapia where SOD, CAT, GPx, GSH and GR activities were declined (Abdelazim et al 2018).…”

Section: Discussionsupporting

confidence: 93%

Study on the impacts of chemical and green synthesized (Leucas aspera and oxy-cyclodextrin complex) dietary zinc oxide nanoparticles in Nile tilapia (Oreochromis niloticus)

Kurian

Elumalai

2021

Environ Sci Pollut Res

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The present study was designed to evaluate the health effects of dietary nanozinc prepared by two methods: conventional chemical method and green method. The parameters evaluated were the extent of bioaccumulation, antioxidant status, histological, immunological changes and DNA damage in Nile tilapia fed nanozinc feed. Zinc oxide nanoparticles were first prepared by green and chemical methods. Before feed preparation, the in vitro antioxidant activity and antibacterial activity of both types of nanoparticle solutions were tested and the results revealed enhanced activities in green synthesized ZnO NP solution. After the acclimatization period, 420 Nile tilapias were distributed randomly into 21 glass tanks with 20 fish per tank in triplicates. Fish were fed control diet without any ZnO NP and (i) GT1—green synthesized ZnO NP diet at 100 mg/kg, (ii) CT1—chemically synthesized ZnO NP diet at 100 mg/kg, (iii) GT2—green synthesized ZnO NP diet at 200 mg/kg, (iv) CT2—chemically synthesized ZnO NP diet at 200 mg/kg, (v) GT3—green synthesized ZnO NP diet at 400 mg/kg and (vi) CT3—chemically synthesized ZnO NP diet at 400 mg/kg for 60 days. After 60 days, gill and liver samples were collected for analysing oxidative stress, histopathological alterations and bioaccumulation of zinc, whereas serum samples were collected for evaluating immune response. The results revealed that the GT3 diet significantly (P < 0.05) enhanced the level of antioxidant enzymes (CAT, SOD, GPx, GR and GSH) than dietary nanozinc prepared by the chemical method. Similarly, the innate immunological parameters were significantly (P < 0.05) augmented in fish fed GT3 diet. Comparative histological study of liver and gill tissues revealed normal architecture in the tissues of fish fed green synthesized NP–enriched feed, whereas the tissues of fish fed chemically synthesized NP feed exhibited histological alterations. Bioaccumulation of zinc was more in the liver followed by the muscle and least in the gills and DNA damage was more evident in fish fed chemically synthesized ZnO NP–enriched feed. In conclusion, the results suggest that the inclusion of 400 mg/kg GT3 diet in fish diet enhanced the level of antioxidant enzymes, boosted immune response and did not cause histological damage to organs, and therefore, GT3 nanofeed can be recommended for fish health improvement.

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Section: Discussionsupporting

confidence: 93%

Study on the impacts of chemical and green synthesized (Leucas aspera and oxy-cyclodextrin complex) dietary zinc oxide nanoparticles in Nile tilapia (Oreochromis niloticus)

Kurian

Elumalai

2021

Environ Sci Pollut Res

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“…Vit C is a highly effective antioxidant and a free radical scavenger in many metabolic processes [21,22]. Vit C increases the percentage of live, acrosome-intact sperm, and decreases the percentage of abnormal sperm during storage at 5 • C. Vit C prevents membrane lipid oxidation during preparation and thus has protective effects.…”

Section: Introductionmentioning

confidence: 99%

Comparison between the Effects of Adding Vitamins, Trace Elements, and Nanoparticles to SHOTOR Extender on the Cryopreservation of Dromedary Camel Epididymal Spermatozoa

Shahin

Khalil

Saadeldin

et al. 2020

Animals

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There are several obstacles in camel semen cryopreservation; such as increasing semen viscosity and the reduction in motile spermatozoa after ejaculation. Epididymal spermatozoa offer an efficient alternative to overcome these problems and are well-suited for artificial insemination in camels. In the current study, we compared the effects of supplementation with vitamin C, E, inorganic trace elements of selenium (Na2SeO3) and zinc (ZnSO4), and zinc and selenium nanoparticles (ZnONPs and SeNPs, respectively) on the cryopreservation of dromedary camel epididymal spermatozoa. When the SHOTOR extender was supplemented with ZnONPs and SeNPs; the sperm showed increased progressive motility; vitality; and membrane integrity after cooling at 5 °C for 2 h; when compared to the control and vitamin-supplemented groups. Moreover, the ZnONPs and SeNPs supplementation improved the progressive motility, vitality, sperm membrane integrity, ultrastructural morphology, and decreased apoptosis when frozen and thawed. SeNPs significantly increased reduced glutathione (GSH), superoxide dismutase (SOD), and decreased lipid peroxide malondialdehyde (MDA) levels. The advantageous effects of the trace elements were potentiated by reduction into a nano-sized particle, which could increase bioavailability and reduce the undesired liberation of toxic concentrations. We recommend the inclusion of SeNPs or ZnONPs to SHOTOR extenders to improve the cryotolerance of camel epididymal spermatozoa.

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“…It has been reported that Zn-containing nanocompounds (such as ZnO NPs) at their toxic effects or doses could raise the concentration of MDA and reduce the GSH contents. Also, they can reduce the activities of antioxidative enzymes, such as SOD, CAT, glutathione reductase (GR), glutathione peroxidase (GPx), and glutathione-S-transferase (GST), which may be used to investigate the toxicity of these compounds in animals [32].…”

Section: Discussionmentioning

confidence: 99%

Toxicity of microwave-assisted biosynthesized zinc nanoparticles in mice: a preliminary study

Salimi

Rahimi

Forootanfar

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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This study was designed to describe the oral acute and subacute toxicities and underlying toxicological mechanisms of biogenic Zn NPs in mice. The Zn NPs were prepared by a green microwave-assisted synthesis method in the presence of Lavandula vera leaf extract. Determination of median lethal dose (LD 50) of Zn NPs and the subacute toxicity after 14 days of exposure was performed as a measurement of substance toxicity through general toxicological, hematological, serum, and histopathological investigations. The western blotting was used to determine the cleaved-caspase-3 expression in the sampled tissues. Flame atomic absorption spectrophotometer (AAS) was applied to estimate the Zn levels in tissues. The SEM analyses revealed that the biogenic Zn NPs were spherical-shaped with the size range of 30-80 nm. The LD 50 value above 5 g/kg indicated that biogenic Zn NPs could be classified as nontoxic chemicals. In subacute toxicity, no significant differences were found in the body weight as well as hematological and oxidative stress (OS) biomarkers after exposure to Zn NPs at the dose of 1 g/kg in comparison to the control. The AAS results indicated that Zn NPs were mainly distributed in the testis, liver, and brain. The findings of histology images of Zn NPs at the dose of 1 g/kg were similar to those of the control. Furthermore, no significant differences were observed in cleaved-caspase-3 expression after exposure to Zn NPs at the dose of 5 g/kg. The results demonstrated that changes in the OS were not related to caspase pathway and the no-observed-adverse-effect level (NOAEL) dose of biogenic Zn NPs in 14-days subacute toxicity study was lower than 1 g/kg.

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Oxidative Stress in the Muscles of the Fish Nile Tilapia Caused by Zinc Oxide Nanoparticles and Its Modulation by Vitamins C and E

Cited by 74 publications

References 60 publications

Study on the impacts of chemical and green synthesized (Leucas aspera and oxy-cyclodextrin complex) dietary zinc oxide nanoparticles in Nile tilapia (Oreochromis niloticus)

Study on the impacts of chemical and green synthesized (Leucas aspera and oxy-cyclodextrin complex) dietary zinc oxide nanoparticles in Nile tilapia (Oreochromis niloticus)

Comparison between the Effects of Adding Vitamins, Trace Elements, and Nanoparticles to SHOTOR Extender on the Cryopreservation of Dromedary Camel Epididymal Spermatozoa

Toxicity of microwave-assisted biosynthesized zinc nanoparticles in mice: a preliminary study

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