Oxidative stress in the rat heart, studies on low‐level chemiluminescence

Abstract

Detection of ultraweak chemiluminescence (CL) emission from the surface of the organ is a sensitive and non-disruptive tool to evaluate the oxidative stress in rat heart. Indeed, an increased photon emission rate can be observed when cellular antioxidants such as glutathione or vitamin E are depleted, or when organic hydroperoxides are infused. We used CL recording to demonstrate in rat heart that: (i) different diets may lead to different heart sensitivity to an oxidative stress; and (ii) post-ischaemic reoxygenation induces an oxidative stress. CL emission induced by an oxidative stress is accompanied by an increased release of eicosanoids. However, while non-steroid anti-inflammatory drugs (aspirin, indomethacin and ibuprofen) prevented eicosanoid release, these compounds dramatically enhanced hydroperoxide-dependent CL. The nature of this phenomenon is still obscure, but the increase of steady-state concentration of excited species caused by anti-inflammatory drugs seems to be pathophysiologically relevant, since in all our experimental conditions tissue damage was proportional to CL emission rate.

Entanglement Between Bio-Photons and Tubulins in Brain: Implications for Memory Storage and Information Processing

“…It is hypothesized that emissions are duo to exited free radicals which emit bio-photons during their de-excitation. This mechanism is supported by many experimental evidences like augmentation of bio-photon emissions by reducing tissue's antioxidants (Ursini et al, 1989) or increased bio-photon emissions after addition of reactive oxygen species (ROS) (Boveris et al, 1980). Even in rat's brain tissue UPE is correlated with cerebral energy metabolism and oxidative stress (Kobayashi et al, 1999).…”

Synthesis and pharmacological evaluation of novel derivatives of anti-inflammatory drugs with increased antioxidant and anti-inflammatory activities

“…Indomethacin administration in vivo (rat) induces a marked increase in TBA reactive substances formation in liver homogenates, as well as in indomethacin-induced gastric mucosal lesions [Tanaka and Yuda, 1996] while it also decreases significantly the level of reduced glutathione in the liver [Naik et al, 1972]. These reports show that treatment with some NSAIDs (at least in rats) is able to increase significantly the susceptibility of liver and other tissues to lipid peroxidation [Parola et al, 1984;Ursini et al, 1989] and further reveal a potentially important role of lipid peroxidation in indomethacin-induced ulceration.…”

Further Observations on Visual Perception: The Influence of Pathologies Upon the Absorption of Light and Emission of Bioluminescence

“…The phenomena of bioluminescence is considered to be associated with the excitation of substrates by enzymatic catalysis or by oxidative stress involving reactive oxygen species [72][73][74][75].…”