Oxidative stress indices in follicular fluid as measured by the thermochemiluminescence assay correlate with outcome parameters in in vitro fertilization

Purpose The purpose of this study was to evaluate the oxidative stress status (OS) of follicular fluid (FF) and the oocyte quality in women with polycystic ovary syndrome (PCOS) undergoing different ovarian stimulation protocols. Methods FF samples were collected after gonadotropin administration in association or not with metformin or D-chiro-inositol (DCI). OS status was then evaluated by checking the follicular fluid protein oxidation profile after specific labeling of aminoacidic free-SH groups, and two-dimensional electrophoresis followed by qualitative and semiquantitative analysis. Oocyte quality was assessed by international morphological criteria. Results Our data indicated that both treatments, even if to different extent, recovered a significantly high level of free-SH groups in FF proteins of PCOS women clearly indicating a decrease of OS level with respect to that found in FF samples from gonadotropins alone treated women. A higher number of good quality MII oocytes was also observed in DCI (P<0.05) or metformin (P<0.05) study groups in comparison to untreated control group. Conclusion A natural supplement and a drug both showed a statistically significant positive effect on follicular milieu by decreasing the oxidative damage on FF proteins, as well as in recovering good quality oocytes.

Section: Discussionmentioning

confidence: 99%

Protein modification as oxidative stress marker in follicular fluid from women with polycystic ovary syndrome: the effect of inositol and metformin

Piomboni

Focarelli

Capaldo

et al. 2014

“…Extensive ROS production in granulosa cells seems to have an adverse effect on oocyte, embryo quality and implantation rate. Higher production of ROS in granulosa cells results in oxidative stress in the follicular fluid; excessive levels of oxidative stress in a follicle have been found to have a negative impact on the outcome of IVF [30].…”

Section: Discussionmentioning

confidence: 99%

Effect of apoptosis and reactive oxygen species production in human granulosa cells on oocyte fertilization and blastocyst development

Jančar

Kopitar

Ihan

et al. 2007

Purpose: The aim was to establish the impact of human granulosa cell apoptosis and reactive oxygen species (ROS) production on fertilization competence of the oocyte, embryo developmental stage and implantation rate.Methods: Thirty women undergoing IVF-ET for tubal factor infertility were included; GnRH antagonists and gonadotrophins were used for ovarian stimulation. Granulosa cells were isolated from each aspirated follicle using gradient centrifugation. Apoptosis was studied by flow cytometry using annexin V and propidium iodide. ROS production was studied with hydroethidine staining and analyzed by flow cytometry.Results: There were no differences in characteristics of granulosa cells between the follicles with fertilized and nonfertilized oocytes. The analyzed characteristics of granulosa cells in corresponding follicles had no effect on embryo developmental stage on day 5. The percentage of ROS producing granulosa cells was lower in the follicles giving rise to blastocysts that resulted in implantation compared to those that did not (39.9% versus 69.9%, P = 0.031).Conclusions: Apoptosis and ROS production in granulosa cells have no significant impact on fertilization and do not correlate with the development of blastocysts. An increased

“…Uncontrolled peroxidation can damage enzymes, lipids, proteins and cell membranes and results in cell injury and death [14]. One of the several consequences of ROS production in the ovary is damage to the plasma membranes through LPO of polyunsaturated fatty acids [15][16][17]. ROS levels and LPO in follicular fluid of the developing oocyte have been suggested as markers of embryo quality in IVF [17].…”

Section: Introductionmentioning

confidence: 99%

Changes of sFas and sFasL, oxidative stress markers in serum and follicular fluid of patients undergoing IVF

Pekel

Gönenç

Turhan

et al. 2014

Purpose The Fas-Fas Ligand interaction is one of the essential events for the induction of apoptosis whereas the exact role of their soluble forms in the reproductive system is still not fully understood. Also oxidative stress in the pathogenesis of infertility causing diseases in women and has been suggested as one of the important factors that negatively affect IVF outcome. In this study, our aim was to evaluate serum and follicular fluid levels of soluble Fas soluble Fas Ligand, malondialdehyde, superoxide dismutase and total antioxidant capacity in patients undergoing IVF and compared with controls. Methods This study included 109 patients. Patients were classified as unexplained infertility (N=31), PCOS (N=19), tubal factor (N=9) and endometriosis (N=10) and compared with male factor infertility (N=40) that was the control group. sFas and sFasL levels were measured by immunoassay method. MDA, SOD and TAC levels were measured by colorimetric method. Results Patients with unexplained infertility, PCOS and tubal factor had significantly lower sFas levels compared with their controls (respectively, p<0.01, p<0.05, p<0.05). However, SOD activity in unexplained infertility, PCOS and endometriosisgroupswere significantly higher than control group (p<0.01).Decreased follicular fluid TAC levels were found in all patient groups compared with controls (respectively, p<0.01, p<0.05, p<0.01, p<0.01).Patients with tubal factor had significantly higher serum sFasL (p<0.05), but lower follicular fluid sFasL levels (p<0.05) compared with unexplained infertility. Tubal factor and endometriosis groups had lowerfollicular fluid TAC levels compared to unexplained infertility and PCOSgroups (p<0.01). Conclusion(s) In this study, serum and follicular fluid sFas levels were decreased and antioxidant activity was impaired in infertility, possibly implying increased apoptosis. Especially in unexplained infertility group changes in this parametres more remarkable.