Oxidative stress-induced impairment of trophoblast function causes preeclampsia through the unfolded protein response pathway

Mukherjee, Indrani; Dhar, Ruby; Singh, Sunil; Sharma, Jai Bhagwan; Nag, Tapas Chandra; Mridha, Asit Ranjan; Jaiswal, Parul; Biswas, Subhrajit; Karmakar, Subhradip

doi:10.1038/s41598-021-97799-y

Cited by 38 publications

(61 citation statements)

References 69 publications

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“…Our long-term MSG treatment however downregulates both invasion and differentiation. This could be accounted as a consequence of an excess of ROS production that seems to exhaust the cellular antioxidant defense systems (as seen by downregulation of Nrf2) leading to loss of invasion potential of these cells 62 or could further lead to their apoptosis 63,64 .…”

Section: Discussionmentioning

confidence: 99%

Monosodium glutamate (MSG) perturbs trophoblast differentiation and invasion program through a ROS-mediated pathway

Mukherjee¹,

Singh

Biswas³

et al. 2022

Preprint

Self Cite

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Monosodium glutamate (MSG) is a flavor-enhancing food additive widely used in Asian cuisine. It is one of the common ingredients used in curry and other tinned food that is believed to bring out the "umami" meat flavor and enhance the savory taste of the food. It is the sodium salt of the naturally occurring amino acid Glutamate. Due to its massive consumption as part of fast-food culture along with concerns about its possible effect on health in general and pregnancy in particular, we undertook this study to address its impact on placental trophoblast cells. We investigated the effect of MSG on placental trophoblast cells. Our studies confirmed that short and long-term exposure to MSG has a profound influence on trophoblast invasion and differentiation, two of the most critical and dispensable functions during placentation. Our results showed an acute short-term MSG-treatment enhanced trophoblast invasion and cell motility along with an increased expression in trophoblast cell differentiation markers. However, long-term chronic MSG stimulation in these cell types showed the opposite effect with both reduced invasion and differentiation. Further, MSG-treated placental explants displayed signs of elevated oxidative stress. These effect of MSG on trophoblast cells along with an increased ROS production indicates that long-term usage of MSG as part of food additive might have adverse health consequences on the developing embryo by compromising the trophoblast and placental function.

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Section: Discussionmentioning

confidence: 99%

Monosodium glutamate (MSG) perturbs trophoblast differentiation and invasion program through a ROS-mediated pathway

Mukherjee¹,

Singh

Biswas³

et al. 2022

Preprint

Self Cite

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“…Preeclampsia is associated with insufficient trophoblast infiltration and impaired vascular remodeling in spiral arteries [64]. Impaired vascular remodeling in preeclampsia reduces placental perfusion and oxygenation, and increases oxidative stress with ROS and toxic lipid peroxides [65].…”

Section: Abnormal Placental Vascular Remodelingmentioning

confidence: 99%

An Update Review of the Pathogenesis Hypothesis in Preeclampsia

Li¹,

Zhu²,

Xi³

2022

Clin. Exp. Obstet. Gynecol.

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Objectives: Hypertensive disorders occur in approximately 12% to 22% of pregnancies and cause substantial perinatal morbidity and mortality of both mother and fetus. Hypertensive disease is directly responsible for approximately 20% of maternal deaths and can be classified as chronic hypertension, gestational hypertension, preeclampsia-eclampsia, and chronic hypertension with superimposed preeclampsia. At present, the pathogenesis of preeclampsia is still unclear, we wrote this article to make a uptodate review of this disease. Mechanism: A comprehensive search of several databases was conducted from inception up to March 2022. The searched databases were Web of Science, MEDLINE,Ovid, and Cochrane Database of Systematic Reviews. The search strategy included the combinations of the following medical terms: Hypertensive disorders; preeclampsia; mechanism; pathogenesis hypothesis. Findings in Brief: At present,the pathogenesis of preeclampsia is still unclear, the theory of Genetic,Inflammatory Response, Immune Imbalance in Maternal-Fetal Interface, Oxidative Stress, Vascular Endothelial Cell Damage are supposed involved in the progress of preeclampsia. Conclusions: Although there are various theories mentioned above, none of the hypothesis can fully explain preeclampsia. More research is needed on the mechanism of preeclampsia.

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“…for 15 mins in the dark at room temperature. 10,000 events/run were acquired, and the results were analyzed using FlowJo software (Ashland, OR, USA) 55 .…”

Section: Cell Cycle and Apoptosis Assaymentioning

confidence: 99%

“…Post MSG treatment, as mentioned above, 5 µM CellRox (Cellular ROS Assay Kit, ab186029S) was added to each well and was kept for incubation for 30 mins at 37°C. The cells were then washed three times with 1X PBS at room temperature and analyzed by ow cytometry using BD FACSCantoTM 55 .…”

Section: Ros Productionmentioning

confidence: 99%

Monosodium glutamate (MSG) perturbs trophoblast differentiation and invasion program through a ROS mediated pathway

Mukherjee¹,

Singh

Biswas³

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

Monosodium glutamate (MSG) is a flavor-enhancing food additive widely used in Asian cuisine. It is one of the common ingredients used in curry and other tinned food that is believed to bring out the "umami" meat flavor and enhance the savory taste of the food. It is the sodium salt of the naturally occurring amino acid Glutamate. Due to its massive consumption as part of fast-food culture along with concerns about its possible effect on health in general and pregnancy in particular, we undertook this study to address its impact on placental trophoblast cells. We investigated the effect of MSG on placental trophoblast cells. Our studies confirmed that short and long-term exposure to MSG has a profound influence on trophoblast invasion and differentiation, two of the most critical and dispensable functions during placentation. Our results showed an acute short-term MSG-treatment enhanced trophoblast invasion and cell motility along with an increased expression in trophoblast cell differentiation markers. However, a long-term chronic MSG stimulation in these cell types showed an opposite effect with both reduced invasion and differentiation. Further, MSG-treated placental explants displayed signs of an elevated oxidative stress. These effect of MSG on trophoblast cells along with an increased ROS production indicates that short and long-term usage of MSG as part of food additive might have adverse health consequences on the fetus by compromising the trophoblast and placental function.

show abstract

Oxidative stress-induced impairment of trophoblast function causes preeclampsia through the unfolded protein response pathway

Cited by 38 publications

References 69 publications

Monosodium glutamate (MSG) perturbs trophoblast differentiation and invasion program through a ROS-mediated pathway

Monosodium glutamate (MSG) perturbs trophoblast differentiation and invasion program through a ROS-mediated pathway

An Update Review of the Pathogenesis Hypothesis in Preeclampsia

Monosodium glutamate (MSG) perturbs trophoblast differentiation and invasion program through a ROS mediated pathway

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