Oxidative stress induces vascular heme oxygenase-1 expression in ovariectomized rats

Lee, Yen-Mei; Cheng, Pao Yun; Hong, Su Fen; Chen, Shu Ying; Lam, Kwok Keung; Sheu, Joen Rong; Yen, Mao‐Hsiung

doi:10.1016/j.freeradbiomed.2005.02.033

Cited by 38 publications

(34 citation statements)

References 49 publications

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“…In the present study, NO release in the mitochondrial fraction was found to be increased in liver isolated from ovariectomized females rats, in accordance with previous studies of our group performed in primary cultures of hepatocytes obtained from old female rats in which the increase was also more marked in ovariectomized animals and with other studies showing also that iNOS activity and NO release were increased with ovariectomy (Lee et al 2005;Koyuncu et al 2006). In addition to stimulating NO levels, our data show that ovariectomy can also decrease cytochrome C content in mitochondrial fraction and increase it in cytosol.…”

Section: Discussionsupporting

confidence: 93%

Effect of chronic treatments with GH, melatonin, estrogens, and phytoestrogens on oxidative stress parameters in liver from aged female rats

et al. 2007

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The aging theory postulates that this process may be due to the accumulation of oxidative damage in cells and molecules. The present study has investigated the effect of castration in old female rats on various parameters related to the antioxidant properties of several cellular fractions obtained from the liver, and the influence of several chronic treatments on it, both in intact and castrated animals. Sixty-one 22-month-old Wistar female rats, were used. About 21 intact animals were divided into three groups and treated for 10 weeks with GH, melatonin or saline, and 40 ovariectomized (at 12 months of age) animals were divided into five groups and treated for the same time with GH, melatonin, estrogens (Eos), phytoestrogens (Phyt) or saline. All animals were sacrificed at 24 months of age by decapitation. The activity of glutathione peroxidase (GPx) in cytosolic fraction, glutathione-S-transferase (GST) in cytosol and microsomal fractions, and the levels of nitric oxide (NO) and cytochrome C in mitochondrial and cytosol fractions of liver were determined. A decrease in GST activity was detected in cytosol and in the microsomal fraction in ovariectomized animals as compared to intact rats. The activity of GPx was also decreased in ovariectomized as compared with the intact group. NO level was increased and cytochrome C decreased in the mitochondrial fraction of the liver in ovariectomized females as compared with the intact group, respectively. No significant changes after melatonin or GH treatments were found in GPx, GST activity and NO level in mitochondrial fraction in the intact group. Administration of GH, melatonin, Eos and Phyt in the ovariectomized groups significantly increased the GPx, and GST activity in the cytosol and microsomal fraction and decreased the level of NO in the mitochondrial fraction as compared with the untreated rats. A significant increase in the level of cytochrome C in the mitochondrial fraction and a decrease in the cytosol fraction were also found with all treatments. The administration of GH, melatonin, Eos and Phyt to castrated females seem to reduce oxidative changes in the liver from old ovariectomized rats.

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Section: Discussionsupporting

confidence: 93%

Effect of chronic treatments with GH, melatonin, estrogens, and phytoestrogens on oxidative stress parameters in liver from aged female rats

et al. 2007

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“…Our results clearly indicated that HO-1 expression was significantly increased in both old intact and castrated females, as compared to young animals, and these data correlated with a parallel increase in the level of pro-inflammatory cytokines and expression of iNOS. These findings are in consonance with the results obtained by Lee et al (2005), in which HO-1 protein expression in heart was induced by ovariectomy and was extremely high 2-6 weeks after castration when compared with the sham-operated group. 17β-Oestradiol replacement after ovariectomy was able to reverse these changes in rats.…”

Section: Discussionsupporting

confidence: 91%

Melatonin and oestrogen treatments were able to improve neuroinflammation and apoptotic processes in dentate gyrus of old ovariectomized female rats

Kireev

Vara

Viña

et al. 2014

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The aim of this study was to determine the outcomes of oestrogen and melatonin treatments following long-term ovarian hormone depletion on neuroinflammation and apoptotic processes in dentate gyrus of hippocampi. Forty-six female Wistar rats of 22 months of age were used. Twelve of them remained intact, and the other 34 were ovariectomized at 12 months of age. Ovariectomized animals were divided into three groups and treated for 10 weeks with oestrogens, melatonin or saline. All rats were killed by decapitation at 24 months of age, and dentate gyri were collected. A group of 2 month-old intact female rats was used as young control. The levels of pro-inflammatory cytokines and heat shock protein 70 (HSP 70) were analysed by ELISA. The expressions of TNFα, IL1β, GFAP, nNOS, iNOS, HO-1, NFκB, Bax, Bad, AIF, Bcl2 and SIRT1 genes were detected by real-time (RT)-PCR. Western blots were used to measure the protein expression of NFκB p65, NFκB p50/105, IκBα, IκBβ, p38 MAPK, MAP-2 and synapsin I. We have assessed the ability of 17β-oestradiol and melatonin administration to downregulate markers of neuroinflammation in the dentate gyrus of ovariectomized female rats. Results indicated that 17β-oestradiol and melatonin treatments were able to significantly decrease expression of pro-inflammatory cytokines, iNOS and HO-1 in the hippocampus when compared to non-treated animals. A similar age-and long-term ovarian hormone depletion-related increase in GFAP was also attenuated after both melatonin and oestradiol treatments. In a similar way to oestradiol, melatonin decreased the activation of p38 MAPK and NFκB pathways. The treatments enhanced the levels of synaptic molecules synapsin I and MAP-2 and have been shown to modulate the pro-antiapoptotic ratio favouring the second and to increase SIRT1 expression. These findings support the potential therapeutic role of melatonin and oestradiol as protective antiinflammatory agents for the central nervous system during menopause.

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“…The administration of estradiol to old ovariectomized rats significantly decreased the protein expression of HO-1, and this fact was accompanied by decreased oxidative stress and pro-inflammatory markers. These findings are in consonance with the results obtained by Lee et al (2005), in which HO-1 protein expression was induced by ovariectomy and was extremely high 2-6 weeks after castration when compared with the sham-operated group. 17b-Estradiol replacement reversed these changes in rats after ovariectomy.…”

Section: Discussionsupporting

confidence: 91%

“…Increasing evidence supports the co-expression of HO and NOS in a variety of cells and tissues, suggesting possible interactions or coordination between the CO-and NO-generating systems (Alm et al 1999;Ho et al 1999). Lee et al (2005) have demonstrated that AG (aminoguanidine), a selective iNOS inhibitor, markedly blocked HO-1 expression in Ovx rats. In the present investigation, administration of estradiol to ovariectomized female significantly abolished iNOS expression and NO x production in liver.…”

Section: Discussionmentioning

confidence: 96%

Hormonal regulation of pro-inflammatory and lipid peroxidation processes in liver of old ovariectomized female rats

et al. 2009

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There is now a large body of evidence suggesting that the decline in ovarian function with menopause is associated with spontaneous increases in pro-inflammatory cytokines. On the other hand, oxidative stress has been implicated in the pathogenesis of several alterations due to menopause, and can arise through the increased production of lipid peroxides (LPO) and/or a deficiency of antioxidant defense. The aim of the present study was to investigate the effect of aging and ovariectomy on various physiological parameters related to inflammation and oxidative stress in livers obtained from old female rats and the influence of chronic exogenous administration of estrogens, phytoestrogens and growth hormone on these. Thirty-six female Wistar rats of 22 months of age were used in the present study. Twelve of them remained intact, and the other 24 had been ovariectomized at 12 months of age. Intact animals were divided into two groups and treated for 10 weeks with GH or saline, and ovariectomized animals were divided into four groups and treated for the same time with GH, estrogens, phytoestrogens or saline. A group of 2 month old intact female rats was used as young control. Protein expression of iNOS, HO-1, IL-6, TNFalpha, and IL-1beta were determined by Western blot analysis. The levels of NO( x ), LPO, TNFalpha, IL-1beta, IL-6 and IL-10 were determined in different fractions of the liver. Levels of LPO in the liver homogenates as well as iNOS protein expression and NO( x ) levels were increased in old rats as compared to young animals; this effect was more evident in ovariectomized animals. Pro-inflammatory cytokines TNF-alpha, IL-1beta and IL-6 were significantly increased and anti-inflammatory IL-10 decreased during ageing and after ovariectomy. Aging also significantly increased expression of HO-1 protein and ovariectomized rats showed an additional increase. Hormonal administration to the ovariectomized groups decreased NO( x ), LPO levels and pro-inflammatory cytokines as compared with untreated rats. Significant rise in IL-10 and reductions in the iNOS, IL-6, TNFalpha and IL-1beta proteins expression were also found. Oxidative stress and inflammation induced during aging in the liver are more marked in castrated than in intact old females. Administration of the different hormonal replacement therapies was able to inhibit the induction of pro-inflammatory cytokines and iNOS, decreased the levels of oxidative stress markers and had therapeutic potential in the prevention of liver injury.

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Oxidative stress induces vascular heme oxygenase-1 expression in ovariectomized rats

Cited by 38 publications

References 49 publications

Effect of chronic treatments with GH, melatonin, estrogens, and phytoestrogens on oxidative stress parameters in liver from aged female rats

Effect of chronic treatments with GH, melatonin, estrogens, and phytoestrogens on oxidative stress parameters in liver from aged female rats

Melatonin and oestrogen treatments were able to improve neuroinflammation and apoptotic processes in dentate gyrus of old ovariectomized female rats

Hormonal regulation of pro-inflammatory and lipid peroxidation processes in liver of old ovariectomized female rats

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