Oxidative Stress Influences Pseudomonas aeruginosa Susceptibility to Antibiotics and Reduces Its Pathogenesis in Host

Zahran, Faten; Shaker, Ghada H.; Askoura, Momen

doi:10.1007/s00284-019-01858-7

Cited by 21 publications

(17 citation statements)

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“…Pseudomonas aeruginosa is a ubiquitous environmental Gram-negative bacterium. It causes several hospital-acquired infections, such as severe systemic infections, endocarditis, ventilator-associated pneumonia, and urinary tract infections [ 1 , 2 ]. As an opportunistic pathogen, P. aeruginosa successfully causes infections in immunocompromised individuals, patients on mechanical ventilation, elderly people, or patients with comorbidities.…”

Section: Pseudomonas Aeruginosa Pathogenicity and Virulencementioning

confidence: 99%

Oxidative Stress Response in Pseudomonas aeruginosa

et al. 2021

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Pseudomonas aeruginosa is a Gram-negative environmental and human opportunistic pathogen highly adapted to many different environmental conditions. It can cause a wide range of serious infections, including wounds, lungs, the urinary tract, and systemic infections. The high versatility and pathogenicity of this bacterium is attributed to its genomic complexity, the expression of several virulence factors, and its intrinsic resistance to various antimicrobials. However, to thrive and establish infection, P. aeruginosa must overcome several barriers. One of these barriers is the presence of oxidizing agents (e.g., hydrogen peroxide, superoxide, and hypochlorous acid) produced by the host immune system or that are commonly used as disinfectants in a variety of different environments including hospitals. These agents damage several cellular molecules and can cause cell death. Therefore, bacteria adapt to these harsh conditions by altering gene expression and eliciting several stress responses to survive under oxidative stress. Here, we used PubMed to evaluate the current knowledge on the oxidative stress responses adopted by P. aeruginosa. We will describe the genes that are often differently expressed under oxidative stress conditions, the pathways and proteins employed to sense and respond to oxidative stress, and how these changes in gene expression influence pathogenicity and the virulence of P. aeruginosa. Understanding these responses and changes in gene expression is critical to controlling bacterial pathogenicity and developing new therapeutic agents.

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Section: Pseudomonas Aeruginosa Pathogenicity and Virulencementioning

confidence: 99%

Oxidative Stress Response in Pseudomonas aeruginosa

et al. 2021

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“…Experiments in PAO1 and clinical isolates showed previously that P. aeruginosa decreases expression of the key quorum-sensing genes (lasI, lasR, rhlI, rhlR, pqsA and pqsR) in response to oxidative stress caused by exposure to H2O2 (65). Considering that quorum sensing regulates hundreds of genes (68), shutting down rhamnolipid secretion could be part of a broader response to reduce oxidative stress, and saves resources for primary metabolism.…”

Section: Discussionmentioning

confidence: 98%

“…7). Others have shown that P. aeruginosa decreases expression of the key quorum-sensing genes (LasI, LasR, RhlI, RhlR, PqsA and PqsR) in response to oxidative stress caused by exposure to H 2 O 2 (Mohamed et al, 2020). Considering quorum sensing regulates hundreds of genes (Lequette et al, 2006), shutting down the cooperative traits that are under quorum-sensing regulation-including rhamnolipid secretion-could save precious resources for oxidative stress responses.…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, in vitro data shows that the expression of genes from the above list, including lasI, lasR, rhlI, rhlR, pqsA, pqsR, in P. aeruginosa PAO1, can be significantly reduced by quorum sensing quencher and ROS molecule H2O2 (65), supporting that the loss of the phenotype may be linked to oxidative stress. More importantly, the zwf gene encoding glucose 6-phosphate dehydrogenase (G6PDH) was expressed higher in non-producers (Supplementary Fig.…”

Section: Since Sdh Contains [2fe-2s] [3fe-4s] and [4fe-4s] Clusters (54) Ros That Damages Fe-s Clusters Could Decrease Sdh Activity In VImentioning

confidence: 97%

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Evolution and regulation of microbial secondary metabolism

Santamaria

Liao

Wang

et al. 2020

Preprint

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Many bacteria have an incredible ability to swarm cooperatively over surfaces. But swarming phenotypes can be quite different even between strains of the same species. What drives this diversity? We compared the metabolomes of 29 clinical Pseudomonas aeruginosa isolates with a range of swarming phenotypes. We identified that isolates incapable of secreting rhamnolipids—a surfactant needed for swarming—had perturbed tricarboxylic acid (TCA) cycle and amino acid pathways and grew exponentially slower in glycerol minimal medium. Analysis of the metabolome signatures and simulations using a genome-scale model led to a mechanism which joins these observations: Strains subject to higher oxidative stress levels grow slower and shut down rhamnolipids secretion, a carbon overflow mechanism possibly to direct carbon resources towards costly stress response pathways to maintain cell viability. In vitro experiments confirmed that rhamnolipid non-producers deal worse with oxidative stress, linking intracellular redox homeostasis—a individual-level trait—to swarming—a population-level behavior. This mechanism helps explain the metabolic constraints on bacteria when secreting byproducts to interact with others—competitively and cooperatively—in microbial communities.SignificanceSwarming motility has been associated with virulence of many human bacterial pathogens. The pathogen Pseudomonas aeruginosa swarms by cooperatively secreting surfactants called rhamnolipids to lubricate surfaces. To understand why some P. aeruginosa strains swarm and others do not, we combined metabolomics, computational modeling and in vitro experiments to study the different swarming behaviors of 29 isolates of Pseudomonas aeruginosa obtained from infected patients. We found that strains can only produce rhamnolipids if they can maintain redox homeostasis. We propose that single cells must have low internal redox stress levels before they can produce rhamnolipids, which work as an overflow of carbon metabolism into a cooperative secretion that brings a fitness benefit to the entire swarming population. This mechanism links single-cell physiology and a population-level cooperative behavior key to the fitness and virulence of P. aeruginosa, a major source of hospital acquired infections.SynopsisThis study combined metabolomics, computational modeling and experiments to explain the swarming diversity in Pseudomonas aeruginosa, yielding new insights on the genetic and metabolic controls of bacterial swarming behaviorRhamnolipid secretion is necessary, but insufficient, for swarmingP. aeruginosa strains unable to produce rhamnolipids in the glycerol minimal medium have perturbed metabolism, slow growth and elevated oxidative stressRhamnolipid producers cannot produce rhamnolipids in succinate as the sole carbon source which causes greater ROS (reactive oxygen species) productionThe requirement of redox homeostasis for rhamnolipid production ensures that the overflow of precious carbon for population-level benefit is prudently controlledThis study helps linking single cell physiology with collective behavior key to the fitness and virulence of a pathogenic bacterium.

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“…(4,5,6) It is a periwinkle grass that has a hollow stem that reaches a length of between (30_100) cm, preferably wrapping the sun . (7,8,9) It can tolerate all soil conditions. (10,11) I ts hermaphroditic flowers are pollinated by insects (bees and flies) and spread by seeds spread by wind and water .…”

Section: Introductionmentioning

confidence: 99%

Effect of Sonchus oleracea Extract on Some Virulence Factor of Klebsiella pneumonia Which Isolated from Urinary Tract Infection in Ramadi Hospital

Hussein,

Khalil,

Qadir Haqi Khali

et al. 2021

Indian Journal of Forensic Medicine & Toxicology

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Background:The plant contains vitamin C, thiamin, riboflavin, and some citrus and saponins. Medical benefits of wrapping Its medicinal benefits are attributed to the similarity between it and chicory Tonic and regulator of sugar level in The blood is a diuretic, a strong purgative, lowers the level of cholesterol in the blood and combats scurvy, as it is anthelmintic Methodology: Klebsiella bacteria were isolated from patients suffering from urinary tract infections in Ramadi General Hospital in selective media (Maconkey Agar), four concentrations of the aqueous and alcoholic extract were made 10%, 20%, 30 % and 40% to test the effectiveness of the extracts in inhibiting growth and virulence factors for bacteria. Result: the effect of the aqueous and alcoholic extracts of the Sonchus oleracea extract on the production of the biofilm of Klebsiella bacteria, where it was observed that there were significant differences in the concentrations of the aqueous and alcoholic extract in reducing the production of the biofilm of the bacteria, and the best concentration of the aqueous and alcoholic extract was 40%, where the biofilm concentration was 31 compared to the rest of the concentrations 3%, 20% and 10%, where its concentration reached .55, .64, and .74, respectively, and compared to the control, where its concentration was .98%. It was not noted that there were significant differences between the concentrations of plant extracts and aqueous and alcoholic extract in Reducing biofilm yield. conclusion the results explain that this plant has many It is one of the effective compounds such as vitamin C, thiamin, riboflavin, and some citrus and saponins. That destroy the cell wall and thus reduce the production of the biofilm and that these compounds increase by increasing the concentration of plant extracts, and this explains the effect of the high concentration in reducing the production of the biofilm.

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Oxidative Stress Influences Pseudomonas aeruginosa Susceptibility to Antibiotics and Reduces Its Pathogenesis in Host

Cited by 21 publications

References 61 publications

Oxidative Stress Response in Pseudomonas aeruginosa

Oxidative Stress Response in Pseudomonas aeruginosa

Evolution and regulation of microbial secondary metabolism

Effect of Sonchus oleracea Extract on Some Virulence Factor of Klebsiella pneumonia Which Isolated from Urinary Tract Infection in Ramadi Hospital

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