Oxidative Stress Levels in Rats Following Exposure to Oxygen at 3 atm for 0–120 min

Öter, Şükrü; Topal, Turgut; Sadir, Serdar; Özler, Mehmet; Uysal, Bülent; Ay, Hakan; Yaren, Hakan; Korkmaz, Ahmet; Akın, Alper

doi:10.3357/asem.2119.2007

Cited by 20 publications

(17 citation statements)

References 27 publications

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“…Almost all of our previous one-session HBO exposure studies also resulted in increased SOD activities in rat erythrocytes (9,11,14). Similarly, we recorded significant elevation of SOD activities in the present work, but only in the early 5 and 10 times HBO administered groups.…”

Section: Discussionsupporting

confidence: 65%

“…Through the last decade, our laboratory has been concentrated on defining the oxidative action of HBO within therapeutically applied limits. In a series of experimental studies conducted in rats, we found that HBO-induced oxidative stress is directly proportional to the exposure pressure (9, 10) and duration (11,12). Another experimental set revealed an important finding that the enhanced levels of oxidation products declined to their baseline values at one hour later following a single HBO exposure (13,14).…”

Section: Introductionmentioning

confidence: 92%

“…Erythrocytes, the oxygen-carrying cells and one of the main targets of hyperoxia-related oxidative action (9,11,14), were chosen for this purpose.…”

Section: Introductionmentioning

confidence: 99%

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Response of Rat Erythrocyte Oxidative Stress Markers to Repetitive Hyperbaric Oxygen Exposures Up to 40 Daily Sessions / Odgovor Markera Oksidativnog Stresa U Eritrocitima Pacova Na Višestruko Izlaganje Hiperbaričnom Kiseoniku Do 40 Seansi Dnevno

Şimşek¹,

Yıldırım²,

Demirbaş³

et al. 2013

Journal of Medical Biochemistry

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SummaryBackground: Studies with single-session hyperbaric oxygen exposures have shown that HBO-induced oxidative stress is proportional to exposure pressure and duration. Since the efficacy of hyperbaric oxygen mainly depends on repetitive exposures, this study aimed to investigate the oxidative effect of hyperbaric oxygen administered for 5 to 40 sessions. Methods: Sixty rats were divided into one control and 6 study groups. Study groups were exposed to 5, 10, 15, 20, 30, and 40 daily consecutive 2.8 atm/90 min hyperbaric oxygen sessions. Animals were sacrificed 24 h after the last hyperbaric oxygen administration. Malondial dehyde and carbonylated protein levels as well as superoxide dismutase activities were determined in isolated rat erythrocytes. Results: Carbonylated protein levels increased significantly after just 5 hyperbaric oxygen exposures; reached a peak level with 10 exposures; were still significantly higher than controls after 15 sessions; and de creased to normal limits after 20 exposures. Malondial dehyde le vels were found to be significantly increased in the 10 to 30, but not in the 5 and 40-session groups. Superoxide dismutase activity showed elevated levels only in the 5 and 10 times hyperbarical oxygenexposed groups. Conclusions: The suppressed oxidative stress level after 40 exposures suggests an effective endogenous antioxidant defense in repetitive HBO administrations.

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Section: Discussionsupporting

confidence: 65%

Section: Introductionmentioning

confidence: 92%

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Response of Rat Erythrocyte Oxidative Stress Markers to Repetitive Hyperbaric Oxygen Exposures Up to 40 Daily Sessions / Odgovor Markera Oksidativnog Stresa U Eritrocitima Pacova Na Višestruko Izlaganje Hiperbaričnom Kiseoniku Do 40 Seansi Dnevno

Şimşek¹,

Yıldırım²,

Demirbaş³

et al. 2013

Journal of Medical Biochemistry

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“…One study assessing oxidative stress levels in rats following exposure to oxygen at 3 atm for 0-120 minutes exhibited a clear relationship of HBO-induced oxidative action to exposure time. This action was most pronounced from 90 to 120 minutes of exposure [29]. Another study on the effects of HBO in rats revealed that after 2 hours of HBO exposure at 3 ATA, levels of the oxidative stress markers elevated in lung, brain and erythrocytes [30].…”

Section: Discussionmentioning

confidence: 99%

Hyberbaric oxygen increases atresia in normal & steroid induced PCO rat ovaries

Atis

Aydın

Ciftci

et al. 2012

Reprod Biol Endocrinol

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BackgroundIn this study, we investigated the effect of hyperbaric oxygen therapy (HBOT) on the morphology of estradiol valerate (EV) induced polycystic ovary (PCO) to find a new treatment modality for improvement of PCO.MethodsThe rats were divided into four groups. Group1, control; group 2, PCO group; group 3, PCO with HBOT group and group 4, normal ovary with HBOT. PCO was induced by a single intramuscular injection of 4 mg EV in adult cycling rats. Other rats with normal ovaries had oil injection as placebo. HBOT was applied to third and fourth groups for six weeks. Histopathologic evaluation of ovaries of all groups were performed & compared.ResultsSix weeks of HBOT was resulted in increase in follicular atresia, decrease in the number of primary, secondary, tertiary follicles and decrease in the number of fresh corpus luteum in normal rat ovary. HBOT on polycystic rat ovary, resulted in significant increase in atretic follicles which were already present.ConclusionsHBOT of six weeks itself, changed ovarian morphology in favor of atresia both in PCO group and control group. This result of aggravated follicular atresia after HBOT on EV induced PCO may be due to long-term exposure in our protocol which with this state seems to be inapplicable in the improvement of PCO morphology.

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“…This study also demonstrated an increase in antioxidant capacity following treatment. Hyperbaric exposure has been shown to cause an increase in oxidative stress in both animal and human experiments (Bearden et al 1999;Otler et al 2007;Benedetti et al 2004), however diVerences in subject population (healthy or diseased), methodology (speciWcally sample type, i.e. tissue, erythrocytes or serum/plasma) and sample timing has an inXuence on the outcome.…”

Section: Discussionmentioning

confidence: 98%

Release of VCAM-1 associated endothelial microparticles following simulated SCUBA dives

et al. 2008

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Microparticles (MP) are shed into the circulation from endothelium following activation or apoptosis. Vascular cell adhesion molecule-1 (VCAM-1) is expressed on endothelial cells following activation and here we report quantification of VCAM-1 positive microparticles (VCAM + MP) following simulated SCUBA dives, breathing either air or oxygen. VCAM + MP were quantified pre-dive (09:00 and 13:00) and post-dive (+1, +3 and +15 h) on both air and oxygen dives and compared with control samples taken from the same subjects. VCAM + MP followed a similar trend in all experiments, however both dives caused a change in endothelial state, as measured by VCAM + MP. A significant increase in VCAM + MP was observed 1 h post-air dive relative to the control (p = 0.013), which was not observed after the oxygen dive (p = 0.095). Oxidative stress (TBARS) was correlated with VCAM + MP. Data presented highlights the potential of MP as a biological marker of both endothelial state and decompression illness.

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Oxidative Stress Levels in Rats Following Exposure to Oxygen at 3 atm for 0–120 min

Abstract: The results of this study exhibited a clear relationship of HBO-induced oxidative action to exposure time. This action was most pronounced from 90 to 120 min of exposure.

Cited by 20 publications

References 27 publications

Response of Rat Erythrocyte Oxidative Stress Markers to Repetitive Hyperbaric Oxygen Exposures Up to 40 Daily Sessions / Odgovor Markera Oksidativnog Stresa U Eritrocitima Pacova Na Višestruko Izlaganje Hiperbaričnom Kiseoniku Do 40 Seansi Dnevno

Response of Rat Erythrocyte Oxidative Stress Markers to Repetitive Hyperbaric Oxygen Exposures Up to 40 Daily Sessions / Odgovor Markera Oksidativnog Stresa U Eritrocitima Pacova Na Višestruko Izlaganje Hiperbaričnom Kiseoniku Do 40 Seansi Dnevno

Hyberbaric oxygen increases atresia in normal & steroid induced PCO rat ovaries

Release of VCAM-1 associated endothelial microparticles following simulated SCUBA dives

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