Oxidative stress markers and thrombomodulin plasma levels in women with early and late severe preeclampsia

Alpoim, Patrícia Nessralla; Perucci, Luíza Oliveira; Godói, Lara Carvalho; Goulart, Cristiano; Dusse, Luci Maria Sant’Ana

doi:10.1016/j.cca.2018.04.039

Cited by 17 publications

(14 citation statements)

References 30 publications

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“…The biochemical events caused by the action of ROS on the unsaturated lipids of cell membranes can be characterized by lipid peroxidation. One of the main markers of oxidative stress for assessing lipid damage is malondialdehyde (MDA), which is formed through the oxidation of polyunsaturated fatty acids, mainly arachidonic acid [13,17,36,37]. Previous studies have reported conflicting results pertaining to MDA levels in pregnancy, Yüksel et al [38] verified a decrease in MDA levels in the third trimester, whereas in contrast, Bukhari et al [31] demonstrated an increase through pregnancy.…”

Section: Discussionmentioning

confidence: 99%

“…This imbalance can occur due to a number of factors such as the over-production of free radicals, overloading the amount of antioxidants available, or an insufficient amount of circulating antioxidants, leading to low body defense against the harmful effects of free radicals [11,12,13]. Due to its great ability to irreversibly oxidize some cellular components such as deoxyribonucleic acid (DNA), carbohydrates, lipids, and proteins, oxidative stress is considered to be one of the most frequent mechanisms associated with the pathophysiology of some diseases as well as some physiological conditions such as gestation [10,14,15,16,17].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, thiol proteins actively participate in redox regulation, being achieved by a combination of cysteine oxidation by hydrogen peroxide or other reactive oxygen species, and the main reducing systems are represented by thioredoxins, glutaredoxins, and peroxiredoxins [18,19]. The sulfhydryl delta-aminolevulinate dehydratase (δ-ALA-D) enzyme that forms part of the heme biosynthetic route in pro-oxidant situations may be inhibited such as preeclampsia and gestational diabetes [10,17,20,21]. This is due to the fact that this enzyme is responsible for catalyzing the asymmetric condensation of two molecules of 5-aminolevulinic acid (ALA), resulting in the formation of the monopyrrole porphobilinogen (PBG).…”

Section: Introductionmentioning

confidence: 99%

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Longitudinal Study of Delta-Aminolevulinate Dehydratase Activity and Oxidative Profile in Healthy Pregnant Women

et al. 2019

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Pregnancy is characterized by changes in various organs, triggering changes in the use of energy substrates and increased oxygen consumption. In addition, gestation is an oxidative event that can be assessed by the relationship between free radicals and antioxidants produced by the body. Excessive production of free radicals has detrimental effects such as damage to enzymes, carbohydrates, and DNA. Thus, the objective of this study was to evaluate the oxidative status and antioxidant responses throughout pregnancy through a longitudinal study. Reactive oxygen species were analyzed by means of thiobarbituric acid reactive substances and nitric oxide, the antioxidant system through vitamin C, sulfhydryl groups, total antioxidant capacity, and ferric reducing ability of plasma as well as enzymes such as catalase and delta-aminolevulinate-dehydratase in pregnant women in the three gestational trimesters (n = 30). According to the results, the markers of oxidative damage showed significant differences in the different gestational trimesters where they were increased in the second trimester when compared to the first trimester. The antioxidant defenses responded differently in each gestational trimester, suggesting a response pattern to try to combat the damage caused by free radicals, in order to stabilize the increase of oxidative stress caused in the second gestational trimester.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Longitudinal Study of Delta-Aminolevulinate Dehydratase Activity and Oxidative Profile in Healthy Pregnant Women

et al. 2019

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“…1,4 Despite the severity of preeclampsia, the precise pathogenesis is not yet fully understood and is currently an area of active research. 4 A series of potential etiologies of preeclampsia, including endothelial dysfunction, 5 excessive in ammation, 6 immunological dysregulation 7 and oxidative stress 8 , has been identi ed. Among these potential etiologies, the imbalance in pro-and anti-in ammatory networks has emerged as the one phenomenon that is most strongly related to the clinical symptoms and disease severity of preeclampsia.…”

Section: Introductionmentioning

confidence: 99%

Vagus Nerve Stimulation Ameliorates L-NAME-induced Preeclampsia-like Symptoms in Rats Through Inhibition of the Inflammatory Response

Zheng

Tang

Shi

et al. 2020

Preprint

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Background Preeclampsia is characterized by an excessive inflammatory response. Recent studies have shown that vagus nerve stimulation (VNS) has anti-inflammatory properties in vivo. This study aims to investigate whether VNS is safe for use during pregnancy and to explore the therapeutic potential and underlying mechanisms of VNS in PE. Methods Pregnant Sprague-Dawley rats were randomly chosen to receive N-nitro-L-arginine methyl ester (L-NAME)-containing water (preeclampsia-like mouse model) or saline (normal pregnancy control) daily at gestational days 14.5-20.5. VNS and the α7nAChR antagonist methyllycaconitine citrate (MLA, 1 mg/kg/d) were given daily at the same time.Results VNS decreased the high systolic blood pressure and urinary protein observed in the PE rats. In addition, VNS mitigated abnormal pregnancy outcomes. Moreover, VNS alleviated the inflammatory response by decreasing the levels of inflammatory cytokines. VNS significantly increased the expression of α7nAChR and attenuated the activation of NF-κB p65 in the placenta.Discussion Our findings indicate that maternal VNS treatment is safe during pregnancy and has a protective effect in a pregnant rat model of preeclampsia induced by L-NAME.

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“…Thrombomodulin (TM) is a transmembrane glycoprotein molecule on the surface of vascular endothelial cells, which can capture thrombin and has an anticoagulant effect by the help of thrombin‐activated protein C system 15 . Although the blood TM levels remained constant from nonpregnant women to normotensive pregnant women, it was higher in early severe preeclampsia women to late severe preeclampsia groups, 16 and it increased significantly from normotensive pregnant to mild pre‐eclampsia to severe pre‐eclampsia 11 . In placenta of patients with preeclampsia, TM levels was higher than pregnant women without pregnancy complications 17 .…”

Section: Introductionmentioning

confidence: 99%

Trimester‐specific reference intervals of TAT, TM, tPAI‐C and PIC for healthy Chinese pregnant women

Qiao

Zhang

et al. 2020

J of Obstet and Gynaecol

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AimPregnancy is a hypercoagulability state, the aim of this study was to observe the changes of thrombin–antithrombin complex (TAT), thrombomodulin (TM), tissue plasminogen activator‐inhibitor complex (tPAI‐C) and plasmin‐α2–antiplasmin complex (PIC) during pregnancy and establish trimester‐specific reference intervals for Chinese healthy pregnant women.MethodsIn total 190 Chinese healthy pregnant women (first trimester 59 cases, second trimester 60 cases and third trimester 71 cases) were recruited in North China. TAT, TM, tPAI‐C and PIC were processed on Sysmex HISCL 5000 automated chemiluminescence immune detection system. Trimester‐specific reference intervals were established with the 2.5th and 97.5th percentile of the distribution.ResultsThe reference intervals for TAT, TM, tPAI‐C, PIC at trimester 1 were 0.40–3.65 ng/mL, 4.85–8.80 TU/mL, 1.75–6.40 ng/mL, 0.25–1.05 μg/mL, respectively. At trimester 2, the reference intervals were 1.65–8.61 ng/mL, 5.70–9.93 TU/mL, 2.91–7.71 ng/mL, 0.33–2.02 μg/mL, respectively. At trimester 3, the reference intervals were 3.16–12.68 ng/mL, 5.50–14.24 TU/mL, 2.70–10.69 ng/mL, 0.24–1.54 μg/mL, respectively.ConclusionsThe changes of TAT, TM, tPAI‐C, PIC during pregnancy are presented, and trimester‐specific reference intervals for healthy pregnant women are described. The levels of TAT, TM, tPAI‐C were increased gradually from trimester 1 to trimester 3, while the PIC level remains stable during all trimesters.

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Oxidative stress markers and thrombomodulin plasma levels in women with early and late severe preeclampsia

Cited by 17 publications

References 30 publications

Longitudinal Study of Delta-Aminolevulinate Dehydratase Activity and Oxidative Profile in Healthy Pregnant Women

Longitudinal Study of Delta-Aminolevulinate Dehydratase Activity and Oxidative Profile in Healthy Pregnant Women

Vagus Nerve Stimulation Ameliorates L-NAME-induced Preeclampsia-like Symptoms in Rats Through Inhibition of the Inflammatory Response

Trimester‐specific reference intervals of TAT, TM, tPAI‐C and PIC for healthy Chinese pregnant women

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