Oxidative stress markers in aqueous humor of glaucoma patients

Ferreira, Sandra M.; Lerner, S.; Brunzini, Ricardo; Evelson, Pablo; Llesuy, Susana

doi:10.1016/s0002-9394(03)00788-8

Cited by 343 publications

(302 citation statements)

References 30 publications

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“…The latter is relevant to neuronal damage in the glaucomatous retina and optic nerve head since it triggers mitochondrial dysfunction and leads to retinal ganglion cell (RGC) death in a cell culture model [7]. The pathogenic role of reactive oxygen species in glaucoma is supported by various experimental findings, such as an increase of the oxidative stress markers superoxide dismutase, catalase, and glutathione peroxidase in the aqueous humor of patients with primary open angle glaucoma and exfoliation glaucoma [8,9]. A significant correlation between oxidative DNA damage in the trabecular meshwork and intraocular pressure (IOP) increase and visual field defects was observed in glaucomatous patients [10].…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial haplogroup U is associated with a reduced risk to develop exfoliation glaucoma in the German population

Wolf

Gramer²,

Müller‐Myhsok

et al. 2010

BMC Genet

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BackgroundVarious lines of evidence demonstrate the involvement of mitochondrial dysfunction in the pathogenesis of glaucoma. Therefore, mitochondrial DNA is a promising candidate for genetic susceptibility studies on glaucoma. To test the hypothesis that mitochondrial haplogroups influence the risk to develop glaucoma, we genotyped 12 single-nucleotide polymorphisms that define the European mitochondrial DNA haplogroups in healthy controls and two German patient cohorts with either exfoliation glaucoma or the normal tension subgroup of primary open angle glaucoma.ResultsMitochondrial haplogroup U was significantly under-represented in patients with exfoliation glaucoma (8.3% compared with 18.9% in controls; p = 0.004).ConclusionsPeople with haplogroup U have a lower risk to develop exfoliation glaucoma. Our results substantiate the suggestion that mitochondrial alterations have an impact on the etiology of glaucoma.

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Section: Introductionmentioning

confidence: 99%

Mitochondrial haplogroup U is associated with a reduced risk to develop exfoliation glaucoma in the German population

Wolf

Gramer²,

Müller‐Myhsok

et al. 2010

BMC Genet

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“…Since the total volume of aqueous humor in the anterior chamber is around 150-200 mL [41], and even decreases with age [42], it is hardly possible to obtain more than 100-150 mL of the sample from one subject [43,44].…”

Section: Aqueous Humormentioning

confidence: 99%

Proteomics in ocular fluids

Grus

Joachim

Pfeiffer

2007

Proteomics Clinical Apps

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The focus of this article is to review recent techniques in proteomic analysis of ocular fluids. These fluids include tears, aqueous humor, and vitreous, they will also be compared to serum analysis. Furthermore, we attempt to summarize some disease correlated biomarkers in ocular fluids that were discovered through different proteomic techniques in eye diseases like dry eye, glaucoma, age-related macular degeneration, uveitis, or diabetic retinopathy. This review is trying to point out the importance of these biomarkers for clinical applications.

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“…It is now well established that excess levels of ocular oxidants can cause damage to the lens and other tissues, which are implicated in normal ageing process and in pathogenesis of several eye diseases, such as pseudoexfoliation syndrome, diabetic retinopathy, glaucoma, and senile cataract (SC). [1][2][3][4][5][6][7] The toxicity of oxidants depends on their ability to induce cross-linking, aggregation, fragmentation, and insolubilization of structural proteins, inactivation of enzymes, and lipid peroxidation (LPO) of the membrane-bound polyunsaturated fatty acids (PUFA), leading to impaired cell function, apoptosis, and necrosis. 1,2 Unlike ocular tissues, AH contains only small quantities of proteins and antioxidant enzymes, and its antioxidant defense thus relies on unusually high levels of low-molecular weight antioxidants, mostly ascorbic acid, present at the level of B1 mM, even in SC.…”

Section: Introductionmentioning

confidence: 99%

“…11 Concentration of oxidants is in AH regulated by a variety of low-molecular as well as enzymatic antioxidants, including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidases (GPx). 4,5,7,12 Although AH is in direct contact with lens surface, little is known about changes in LPO markers and antioxidants caused by the progression of SC. Considering that oxidative stress markers in SC are often compared with other eye diseases, and that AH pathway may be used for targeting ocular antioxidant defense, we aimed this study at assessing the impact of SC maturity on AH LPO markers and antioxidants.…”

Section: Introductionmentioning

confidence: 99%

Influence of cataract maturity on aqueous humor lipid peroxidation markers and antioxidant enzymes

Mirić¹,

Kisić²,

Zoric

et al. 2013

Eye

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Purpose The impact of cataract maturity on the aqueous humor (AH) oxidant/antioxidant balance is largely controversial. This study was aimed at assessing the relationships between cataract maturity and AH lipid peroxidation markers and enzymatic antioxidants. Patients and methods The concentrations of conjugated dienes (CD), lipofuscin-like fluorescent end-products (LLF), soluble proteins, as well as the activities of superoxide dismutase (SOD) and catalase (CAT) were measured in AH samples from nondiabetic patients with either immature (n ¼ 15) or mature (n ¼ 15) cataract. Results In the overall AH sample, the mean values of CD, LLF, SOD, and CAT were 0.160±0.024 (OD 234 ), 166±27 RFU, 24.5±7.1 U/ml, and 31.9±3.9 pmol/ml, respectively. CD was positively correlated with SOD (r ¼ 0.647; Po0.001), CAT (r ¼ À 0.394; P ¼ 0.031), and LLF (r ¼ À 0.399; P ¼ 0.029). The LLF was negatively correlated with SOD (r ¼ À 0.461; P ¼ 0.010). In samples adjusted for confounding factors, differences between immature and mature cataract groups regarding SOD, CD, LLF, and total proteins were significant (Po0.05; for all variables). The multiple logistic regression analysis identified LLF (OR ¼ 4.08; P ¼ 0.038) and SOD (OR ¼ 4.99; P ¼ 0.031) as independent predictors of cataract maturity. Conclusions These results suggest that AH lipid peroxidation markers and antioxidants may significantly depend on the cataract maturity stage.

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Oxidative stress markers in aqueous humor of glaucoma patients

Cited by 343 publications

References 30 publications

Mitochondrial haplogroup U is associated with a reduced risk to develop exfoliation glaucoma in the German population

Mitochondrial haplogroup U is associated with a reduced risk to develop exfoliation glaucoma in the German population

Proteomics in ocular fluids

Influence of cataract maturity on aqueous humor lipid peroxidation markers and antioxidant enzymes

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