Oxidative stress markers in intestinal mucosa of Tunisian inflammatory bowel disease patients

Abstract

Background/Aims:Inflammatory bowel diseases (IBDs), Crohn's disease (CrD) and ulcerative colitis (UC), are chronic gastrointestinal inflammatory disorders. The precise etiology of IBD remains unclear, and it is thought that interactions among various factors, including, genetic factors, the host immune system and environmental factors, cause disruption of intestinal homeostasis, leading to dysregulated inflammatory responses of the gut. As inflammation is intimately related to formation of reactive intermediates, including, reactive oxygen species, oxidative stress has been proposed as a mechanism underlying the pathophysiology of IBD. The purpose of this study is to examine the lipid peroxidation, protein oxidation and anti-oxidative profile in Tunisian IBD.Materials and Methods:Malondialdehyde (MDA), conjugated dienes (CD), protein thiol levels, as well as the catalase (CAT) activity were evaluated in intestinal biopsies of 17 patients affected by IBD (12 CrD and 5 UC) and 12 healthy control individuals.Results:Oxidative stress was confirmed in these two types of disease biopsies as compared to controls. MDA and CD levels were significantly increased in both UC and CrD patients’ biopsies as compared to controls’ biopsies (P < 0.001). CAT activity was similar in UC and CrD biopsies’ and was not significantly increased in IBD patients’ biopsies compared with controls’ biopsies (P > 0.05). Anon-significant decrease in thiol (SH) level was observed in both UC and CrD patients’ biopsies compared with controls’ biopsies (P > 0.05).Conclusion:Increased levels of MDA and CD in IBD patients’ biopsies underline the implication of oxidative stress in the physiopathology of IBD.

Apolipoprotein A-I mimetics mitigate intestinal inflammation in a COX2-dependent inflammatory disease model

“…Apoa1 expression is inhibited in this region (Supplemental Figure 14), as occurs in human CD (38). Lipid peroxidation markers are elevated in plasma and inflamed intestine ( Figure 6), as in human CD (55,56). However, unlike human CD (38), expression of Duox2 was not increased in the terminal ilea of diseased mice (Supplemental Figure 22).…”

Effect of Glucans from Caripia montagnei Mushroom on TNBS-Induced Colitis

“…Oxidative stress is considered a potential etiological factor for Crohn’s disease [37]. Then, the antioxidant system is usually used to evaluate the protective effect of various compounds in colonic inflammation.…”

Triphala, an Ayurvedic formulation improves the antioxidant status on TNBS induced IBD in rats

“…3). Bouzid et al [44] studied IBD in Tunisian population, wherein increased MDA levels were observed in tissue biopsies. Triphala, with its strong antioxidant potency can reverse the oxidative stress, reduce inflammation and thus restores the integrity of the gastrointestinal barrier function.…”