Oxidative stress of glyphosate, AMPA and metabolites of pyrethroids and chlorpyrifos pesticides among primary school children in Cyprus

Makris, Konstantinos C.; Efthymiou, Nikolaos; Konstantinou, Corina; Anastasi, Elena; Schoeters, Greet; Kolossa-Gehring, Marike; Katsonouri, Andromachi

doi:10.1016/j.envres.2022.113316

Cited by 29 publications

(10 citation statements)

References 27 publications

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“…Findings from our study and several other human population studies to date ( 27 , 28 , 42 , 43 ) agree with in vitro and animal studies that have together provided strong evidence for the potential of glyphosate to induce oxidative stress ( 12 , 24 ). In particular, rodent studies have shown increased lipid peroxidation upon glyphosate exposure, as indicated by elevated MDA levels in blood or tissues of glyphosate-treated animals relative to untreated animals ( 12 , 24 ).…”

Section: Discussionsupporting

confidence: 91%

“…Furthermore, although our study focused on occupational exposure in farmers, we also observed associations between urinary glyphosate and oxidative stress biomarkers, particularly 8-OHdG, among nonfarmers, suggesting these effects may apply more broadly to the general population who are primarily exposed through ingestion of contaminated food and water or residential applications ( 8 ). Two prior general population studies, one conducted among pregnant women ( 42 ) and the other among children ( 43 ), have examined glyphosate and oxidative stress biomarkers, both reporting positive associations for urinary glyphosate or its main metabolite, aminomethylphosphonic acid (AMPA).…”

Section: Discussionmentioning

confidence: 99%

“…Recent research also suggests that people may be primarily exposed to AMPA through food and water and to a lesser extent from metabolism of glyphosate ( 49 ). Nevertheless, future studies may consider assessing AMPA given experimental evidence of its potential role in oxidative stress ( 12 ) and associations observed with oxidative stress biomarkers in 2 recent general population studies ( 42 , 43 ). Lastly, although we measured 3 established and representative biomarkers of oxidative DNA damage or lipid peroxidation ( 21 ), they may not reflect the full extent of oxidative stress responses; future work using untargeted approaches may uncover additional oxidative stress–related metabolites or pathways associated with glyphosate exposure ( 51 , 68 ).…”

Section: Discussionmentioning

confidence: 99%

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Glyphosate exposure and urinary oxidative stress biomarkers in the Agricultural Health Study

Chang

Andreotti

Ospina

et al. 2023

JNCI: Journal of the National Cancer Institute

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Background Glyphosate is the most widely applied herbicide worldwide, and its use has been associated with increased risks of certain hematopoietic cancers in epidemiologic studies. Animal and in vitro experiments suggest that glyphosate may induce oxidative stress, a key characteristic of carcinogens; however, evidence in human populations remains scarce. We investigated associations between glyphosate exposure and urinary oxidative stress biomarkers in the Biomarkers of Exposure and Effect in Agriculture study, a molecular epidemiologic subcohort in the Agricultural Health Study. Methods This analysis included 268 male farmers selected based on self-reported recent and lifetime occupational glyphosate use and 100 age- and geography-matched male non-farmers. Concentrations of glyphosate and oxidative stress biomarkers (8-hydroxy-2’-deoxyguanosine [8-OHdG], 8-iso-prostaglandin-F2α [8-isoprostane], and malondialdehyde [MDA]) were quantified in first-morning-void urine. We performed multivariable linear regression to evaluate associations of urinary glyphosate and self-reported glyphosate use with each oxidative stress biomarker. Results Urinary glyphosate concentrations were positively associated with levels of 8-OHdG (highest vs. lowest glyphosate quartile; geometric mean ratio [GMR]=1.15, 95% confidence interval [CI]=1.03-1.28, Ptrend=.02) and MDA (GMR = 1.20, 95% CI = 1.03-1.40, Ptrend=.06) overall. Among farmers reporting recent glyphosate use (last 7 days), use in the previous day was also associated with significantly increased 8-OHdG and MDA levels. Compared with non-farmers, we observed elevated 8-isoprostane levels among farmers with recent, high past 12-month, or high lifetime glyphosate use. Conclusions Our findings contribute to the weight of evidence supporting an association between glyphosate exposure and oxidative stress in humans and may inform evaluations of the carcinogenic potential of this herbicide.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Glyphosate exposure and urinary oxidative stress biomarkers in the Agricultural Health Study

Chang

Andreotti

Ospina

et al. 2023

JNCI: Journal of the National Cancer Institute

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“…23−26 A significant association of urinary 3-PBA with oxidative stress biomarkers was observed recently in humans. 27,28 However, whether oxidative stress mediates the effects of pyrethroids on the development of GDM in humans is still unclear.…”

Section: Introductionmentioning

confidence: 99%

“…These toxic effects of oxidative stress may lead to an inadequate insulin response to glucose challenge, resulting in insulin resistance and the development of GDM. Pyrethroids have been found to increase the level of reactive oxygen species (ROS) and cause oxidative damage to biomacromolecules in cells and animals. − A significant association of urinary 3-PBA with oxidative stress biomarkers was observed recently in humans. , However, whether oxidative stress mediates the effects of pyrethroids on the development of GDM in humans is still unclear.…”

Section: Introductionmentioning

confidence: 99%

First Evidence of the Associations of Exposure to Pyrethroid Insecticides with the Risk of Gestational Diabetes Mellitus

Ma,

Song,

et al. 2024

Environ. Sci. Technol. Lett.

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Pyrethroids, a class of extensively used insecticides, may pose health risks to humans. Whether pyrethroid exposure contributes to the development of gestational diabetes mellitus (GDM) in pregnant women remains unknown. The potential association of urinary concentrations of pyrethroid metabolites [3-phenoxybenzoic acid (3-PBA) and 4-fluoro-3-phenoxybenzoic acid] with the risk of GDM was assessed using a nested case-control study of 220 GDM cases and 440 controls from a prospective cohort. The mediation roles of oxidative stress biomarkers [advanced glycation end products (AGEs) and malondialdehyde (MDA)] in the associations were investigated. Urinary concentrations of 3-PBA in these cases were significantly higher than those of the controls. Urinary 3-PBA was positively associated with the risk of GDM. The adjusted odds of GDM significantly increased by 32% in each unit increment of the ln-transformed concentration of urinary 3-PBA. The significant dose−response relationships of GDM with serum AGEs (P trend = 0.03) and MDA (P trend < 0.001) were observed. However, there were no significant correlations between 3-PBA and AGEs or MDA, nor was there a significant mediation effect of AGEs or MDA on the association of 3-PBA with GDM. We provide the first evidence that early gestational exposure to pyrethroids is an environmental risk factor for GDM.

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The situation of chlorpyrifos in Mexico: a case study in environmental samples and aquatic organisms

Ruíz-Arias

Medina-Díaz

Bernal-Hernández

et al. 2023

Environ Geochem Health

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Oxidative stress of glyphosate, AMPA and metabolites of pyrethroids and chlorpyrifos pesticides among primary school children in Cyprus

Cited by 29 publications

References 27 publications

Glyphosate exposure and urinary oxidative stress biomarkers in the Agricultural Health Study

Glyphosate exposure and urinary oxidative stress biomarkers in the Agricultural Health Study

First Evidence of the Associations of Exposure to Pyrethroid Insecticides with the Risk of Gestational Diabetes Mellitus

The situation of chlorpyrifos in Mexico: a case study in environmental samples and aquatic organisms

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