Oxidative stress reduces the muscarinic receptor function in the urinary bladder

Jongh, Rik de; Haenen, Guido R.M.M.; Koeveringe, Gommert van; Dambros, Míriam; Mey, Jo G. R. De; Kerrebroeck, Philip E. V. A. Van

doi:10.1002/nau.20298

Cited by 35 publications

(34 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Functional in vitro studies show that application of RNOS damages the bladder contractile system. 22,23 This correlates with the reduced detrusor contractility found in severely obstructed bladders. In our study of moderately obstructed bladders, the contractile system of the smooth muscle was not impaired, however a denervation was observed.…”

Section: Discussionmentioning

confidence: 91%

“…Surprisingly, cholinergic nerves seem to be largely unaffected by in vitro exposure of the detrusor muscle to RNOS. 22,23 These conflicting data might be explained by the duration of in vitro experiments which may be too short for mediating nerve damage. Furthermore, oxidative stress imposed on the bladder muscle in vitro has a more acute nature than the oxidative stress that occurs in vivo.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Partial bladder outlet obstruction reduces the tissue antioxidant capacity and muscle nerve density of the guinea pig bladder

Jongh

Dambros

Haenen

et al. 2008

Neurourology and Urodynamics

Self Cite

View full text Add to dashboard Cite

This study demonstrates that the antioxidant status of guinea pig bladders exposed to outlet obstruction decreased which might be associated with the observed reduction in nerve density. The results strengthen the hypothesis that oxidative stress is involved in the pathophysiology of bladder dysfunction related to obstructed bladders. Neurourol. Urodynam. 28:461-467, 2009. (c) 2008 Wiley-Liss, Inc.

show abstract

Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Partial bladder outlet obstruction reduces the tissue antioxidant capacity and muscle nerve density of the guinea pig bladder

Jongh

Dambros

Haenen

et al. 2008

Neurourology and Urodynamics

Self Cite

View full text Add to dashboard Cite

show abstract

“…15,[17][18][19][21][22][23]44 In order to avoid animal experiments we decided to use bladders from freshly killed animals from the slaughterhouse. From the logistical point of view we had the immense advantage that the extraction of an abundant number of bladders every day took a matter of minutes.…”

Section: Discussionmentioning

confidence: 99%

“…14 For any in vitro model to be clinically relevant it is imperative that these factors be taken into consideration during model development. Much of the knowledge of urinary bladder function comes from in vitro muscle strips studies, [15][16][17][18][19][20][21][22][23] which have a functional utility limited by the fact that strip contraction can only be recorded in one plane: results do not reflect the potentially complex interactions that underlie the pressure-volume relationship of the intact bladder. 24,25 The pig detrusor is considered an appropriate model for investigating the physiology of bladder function, as the pig has comparable urodynamic chracteristics to that of humans.…”

Section: Introductionmentioning

confidence: 99%

The extracorporeal perfusion of the female pig detrusor as an experimental model for the study of bladder contractility

Dittrich

Beckmann

Maake

et al. 2007

Neurourology and Urodynamics

View full text Add to dashboard Cite

show abstract

“…Another study shows that ethanol induces lipid peroxidation and reduces vitamin E levels in Guinea pigs (22). Furthermore, it has also been described that oxidative stress alters pig bladder contractility, affecting the muscarinic receptor (43).…”

Section: Discussionmentioning

confidence: 94%

Trolox reduces the effect of ethanol on acetylcholine-induced contractions and oxidative stress in the isolated rabbit duodenum

Gonzálo

Grasa

et al. 2011

Rev. esp. enferm. dig.

View full text Add to dashboard Cite

Trolox is a hydrophilic analogue of vitamin E and a free radical scavenger. Ethanol diminishes the amplitude of spontaneous contractions and acetylcholine (ACh)-induced contractions in rabbit duodenum. The aim of this work was to study the effect of trolox on the alterations induced by ethanol on contractility and lipid peroxidation in the duodenum. The duodenal contractility studies in vitro were carried out in an organ bath and the levels of malondialdehyde and 4-hydroxyalkenals (MDA+4-HAD) were measured by spectrophotometry. Trolox increased the reduction induced by ethanol on the amplitude of spontaneous contractions in longitudinal muscle but not in circular muscle. Trolox 4 mM decreased the effects of ethanol on ACh-induced contractions and on MDA+4-HDA concentrations. We conclude that trolox might prevent oxidative stress induced by ethanol in the duodenum. INTRODUCTIONAlcohol is a hydroxyl compound that can be present in the diet. The amount and type of alcohol consumed and the frequency of its consumption can vary tremendously and can have divergent effects on an organism (1,2). The deleterious effects of alcohol, at least partly, involve alcohol induced oxidative injury that has been documented by measurement of oxidant radicals (3-5). The organism has developed a complex defense system which is composed of free radical scavenger molecules such as vitamins and antioxidative enzymes.Ethanol induces inhibition of esophageal, gastric and intestinal contractility (6-9). The inhibition produced by ethanol is attributed to an inhibition of Ca 2+ influx in the canine jejunal circular muscle and in human and cat esophageal contractility (7,8). Alcohol also inhibits the motility of the sphincter of Oddi in vitro (10,11) and the colon (12). In a previous study, we demonstrated the inhibitory effects of ethanol on the spontaneous contractions and ACh-induced contractions in rabbit duodenum, which are reduced by Ca 2+ -activated K + channel antagonists (13). Ethanol also provokes an inhibition of gastric emptying and small intestinal transit that is mediated by type A CCK receptors or capsaicin-sensitive neural pathways (14-16). However, ethanol produces contractile actions in guinea pig gastric smooth muscle, which required extracellular calcium and are blocked by tyrosine kinase inhibitors (17).Trolox, a phenolic antioxidant, originally designed for food preservation, has a chromane structure similar to atocopherol or vitamin E (18,19). Trolox decreases hepatocellular damage during sepsis (20) and abrogates oxidative stress during cold small bowel storage (21).Although antioxidants can attenuate alcohol-induced injury, there is no single antioxidant that protects all organs during all modes of exposure (3). There is very little documentation available on the effect of trolox on intestinal motility. In addition, alcohol induces oxidative stress and REV ESP ENFERM DIG (Madrid) Vol. 103. N.° 8, pp. 396-401, 2011 Received: 10-01-11. Accepted: 18-03-11.

show abstract

Oxidative stress reduces the muscarinic receptor function in the urinary bladder

Abstract: The results of our study demonstrate that oxidative stress induced by CHP affects pig bladder contractility. The muscarinic receptor signaling system is severely damaged. L-type calcium channels and the contractile system are less affected and cholinergic nerves remain largely unaffected.

Cited by 35 publications

References 37 publications

Partial bladder outlet obstruction reduces the tissue antioxidant capacity and muscle nerve density of the guinea pig bladder

Partial bladder outlet obstruction reduces the tissue antioxidant capacity and muscle nerve density of the guinea pig bladder

The extracorporeal perfusion of the female pig detrusor as an experimental model for the study of bladder contractility

Trolox reduces the effect of ethanol on acetylcholine-induced contractions and oxidative stress in the isolated rabbit duodenum

Contact Info

Product

Resources

About