Oxidative stress-related responses at transcriptional and enzymatic levels after exposure to Cd or Cu in a multipollution context

Smeets, Karen; Opdenakker, Kelly; Remans, Tony; Sanden, Suzy Van; Belleghem, Frank Van; Semane, Brahim; Horemans, Nele; Guisez, Yves; Vangronsveld, Jaco; Cuypers, Ann

doi:10.1016/j.jplph.2009.06.014

Cited by 135 publications

(90 citation statements)

References 43 publications

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“…Transportation of toxic metals to safe sequestration sites within the plant cell not only ensures a sound cellular environment but also presents an ideal perspective to abolish the toxic metals from environment by the process of bioremediation and phytoremediation (Peuke and Rennenberg 2005). The reduction of the free metal content within the plant cell can be achieved by two ways: the synthesis of specific chelators and the sequestration of metal complexes (Smeets et al 2009;Cuypers et al 2010). The high affinity for metals imparted by the GSH thiol (-SH) group, coupled with the fact that GSH is a precursor of PCs.…”

Section: Toxic Metals/metalloidsmentioning

confidence: 99%

Glutathione in plants: biosynthesis and physiological role in environmental stress tolerance

Hasanuzzaman

Nahar

Anee

et al. 2017

Physiol Mol Biol Plants

618

284

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Glutathione (GSH; c-glutamyl-cysteinyl-glycine) is a small intracellular thiol molecule which is considered as a strong non-enzymatic antioxidant. Glutathione regulates multiple metabolic functions; for example, it protects membranes by maintaining the reduced state of both a-tocopherol and zeaxanthin, it prevents the oxidative denaturation of proteins under stress conditions by protecting their thiol groups, and it serves as a substrate for both glutathione peroxidase and glutathione S-transferase. By acting as a precursor of phytochelatins, GSH helps in the chelating of toxic metals/metalloids which are then transported and sequestered in the vacuole. The glyoxalase pathway (consisting of glyoxalase I and glyoxalase II enzymes) for detoxification of methylglyoxal, a cytotoxic molecule, also requires GSH in the first reaction step. For these reasons, much attention has recently been directed to elucidation of the role of this molecule in conferring tolerance to abiotic stress. Recently, this molecule has drawn much attention because of its interaction with other signaling molecules and phytohormones. In this review, we have discussed the recent progress in GSH biosynthesis, metabolism and its role in abiotic stress tolerance.

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Section: Toxic Metals/metalloidsmentioning

confidence: 99%

Glutathione in plants: biosynthesis and physiological role in environmental stress tolerance

Hasanuzzaman

Nahar

Anee

et al. 2017

Physiol Mol Biol Plants

618

284

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“…The plasma membrane-associated NADPH oxidase is one of the main sources of ROS produced under Cd toxic conditions (Romero-Puertas et al, 2004;Smeets et al, 2009). To determine whether this enzyme is a major Figure 1.…”

Section: Peroxule Formation and Peroxisomal Movement Are Regulated Bymentioning

confidence: 99%

Peroxisomes Extend Peroxules in a Fast Response to Stress via a Reactive Oxygen Species-Mediated Induction of the Peroxin PEX11a

et al. 2016

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Peroxisomes are highly dynamic and metabolically active organelles that play an important role in cellular functions, including reactive oxygen species (ROS) metabolism. Peroxisomal dynamics, such as the proliferation, movement, and production of dynamic extensions called peroxules, have been associated with ROS in plant cells. However, the function and regulation of peroxules are largely unknown. Using confocal microscopy, we have shown that treatment of Arabidopsis leaves with the heavy metal cadmium produces time course-dependent changes in peroxisomal dynamics, starting with peroxule formation, followed by peroxisome proliferation, and finally returning to the normal morphology and number. These changes during Cd treatment were regulated by NADPH oxidase (C and F)-related ROS production. Peroxule formation is a general response to stimuli such as arsenic and is regulated by peroxin 11a (PEX11a), as Arabidopsis pex11a RNA i lines are unable to produce peroxules under stress conditions. The pex11a line showed higher levels of lipid peroxidation content and lower expression of genes involved in antioxidative defenses and signaling, suggesting that these extensions are involved in regulating ROS accumulation and ROSdependent gene expression in response to stress. Our results demonstrate that PEX11a and peroxule formation play a key role in regulating stress perception and fast cell responses to environmental cues.Peroxisomes are highly versatile organelles that adapt to changes in their cellular environment through morphological and metabolic adjustments (Hu et al., 2012;Sandalio et al., 2013). Plant peroxisomes perform essential functions such as photorespiration and fatty acid b-oxidation, ureide, and phytohormone (auxin and jasmonic acid) metabolisms, and also act as a source of signal molecules such as reactive oxygen and nitrogen species (ROS and RNS; Sandalio and RomeroPuertas, 2015). Peroxisomes contain a large battery of antioxidants to control ROS and RNS accumulation (Sandalio and Romero-Puertas, 2015). These organelles proliferate in response to environmental cues through a complex process involving elongation, constriction, and fission (Hu et al., 2012;Baker and Paudyal, 2014). Deciphering the signaling pathways governing the regulation of peroxisome proliferation under different environmental and metabolic conditions presents a major challenge in this field of research. Before peroxisomal division, organelle elongation occurs in a process regulated by peroxins 11 (PEX11), with the final division requiring dynamin-like or dynamin-related proteins and fission proteins (Hu et al., 2012, Schrader et al., 2012. There is evidence to suggest that ROS are involved in regulating peroxisome proliferation, as some peroxisomal biogenesis genes (PEX) are transcriptionally regulated by H 2 O 2 in both plant and animal cells (López-Huertas et al., 2000). The formation of peroxisomal extensions, known as peroxules, has also been observed to be regulated by exogenous applications of H 2 O 2 (Sinclair et al., 2009;Ba...

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“…CAO catalyzes the oxidative amination of primary amines to aldehydes, with production of hydrogen peroxide. Reactive oxygen species (ROS) can function as signalling molecules in the response to stress (Neill et al 2002;Smeets et al 2009). In plants, hydrogen peroxide induced by CAO is involved in wound healing (Angelini et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis by cDNA-AFLP of Suillus luteus Cd-tolerant and Cd-sensitive isolates

et al. 2010

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The ectomycorrhizal basidiomycete Suillus luteus (L.:Fr.), a typical pioneer species which associates with young pine trees colonizing disturbed sites, is a common root symbiont found at heavy metal contaminated sites. Three Cd-sensitive and three Cd-tolerant isolates of S. luteus, isolated respectively from non-polluted and a heavy metal-polluted site in Limburg (Belgium), were used for a transcriptomic analysis. We identified differentially expressed genes by cDNA-AFLP analysis. The possible roles of some of the encoded proteins in heavy metal (Cd) accumulation and tolerance are discussed. Despite the high conservation of coding sequences in S. luteus, a large intraspecific variation in the transcript profiles was observed. This variation was as large in Cd-tolerant as in sensitive isolates and may help this pioneer species to adapt to novel environments.

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Oxidative stress-related responses at transcriptional and enzymatic levels after exposure to Cd or Cu in a multipollution context

Cited by 135 publications

References 43 publications

Glutathione in plants: biosynthesis and physiological role in environmental stress tolerance

Glutathione in plants: biosynthesis and physiological role in environmental stress tolerance

Peroxisomes Extend Peroxules in a Fast Response to Stress via a Reactive Oxygen Species-Mediated Induction of the Peroxin PEX11a

Transcriptome analysis by cDNA-AFLP of Suillus luteus Cd-tolerant and Cd-sensitive isolates

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