Oxidative stress status of Turkish welders

Abstract

Welding, a fabrication process that joins metals or thermoplastics by causing coalescence, is indispensable in modern society and ubiquitous in industry. Welding generates fumes that contain several metals and gases that comprise fine and ultrafine particles with the potential for adverse effects. Although health risks of welders have been evaluated in different populations, occupational exposure to welding fumes is still considered to be an important health problem, especially in developing countries. The aim of this study was to investigate the effects of welding fume exposure on important oxidative stress parameters such as superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), total glutathione (GSH), glutathione peroxidase (GPx), malondialdehyde (MDA), and 8-hydroxy-2′-deoxyguanosine (8-OHdG) in Turkish welders ( n = 48). The influence of confounding factors such as age, smoking habits, alcohol consumption, and duration of exposure on the studied parameters was also analyzed. In our study, significant decreases in the levels of GSH and activities of CAT, SOD, and GPx and significant increases of MDA, 8-OHdG levels and GR activity were found in the workers compared to the controls. There was a negative correlation between GSH levels and alcohol usage. Also, older workers (≥35 years) had significantly higher GR levels than younger workers. But smoking and alcohol usage, duration of exposure, and utilization of protective measures had no significant effect on the studied parameters in the workers. These results indicate that occupational exposure to welding fumes appears to induce oxidative stress and oxidative DNA damage.

Alleviating Effects of Black Soybean Peptide on Oxidative Stress Injury Induced by Lead in PC12 Cells via Keap1/Nrf2/TXNIP Signaling Pathway

“…The results showed that these three black soybean peptides we screened out could alleviate the abnormal production of Pb-induced ROS and restore cells to a certain extent. Guo et al have shown that Pb 2+ -induced damage propagates through an intercellular junction communication (GJIC) between PC12 cells while inducing apoptosis in bystander cells through ROS mitochondrial-dependent apoptosis signals [ 23 ]. Yuan et al said SOD and GSH/GSSG quickly cleared the ROS within cells to keep the stability of the internal structures of the cells and tissue.…”

“…It is also a basic electron donor for GPx enzymes. GR is a flavin protein whose function is to catalyze NADPH-dependent reduction of GSSG to GSH, which is necessary to maintain adequate cellular GSH contents [ 23 ]. GSH-Px can catalyze glutathione from decreased GSH to oxidized GSSG, and GSH consumption and GSSG production reflect the degree of intracellular oxidation.…”

“…The experiment was used to determine the activity of GR with a GR analysis kit. The principle of this kit is that NADPH can reduce the expression of GSSG at 340 nm when GR is present, measured 5 times every 1 min, and the results are expressed in nmol/min/mL [ 23 ].…”

Effects of Exposure to Metal Fumes on the Reproductive Health of Male Welders

“…A Turkish study on the correlation between serum metal levels and oxidative stress biomarkers among welders identified a clear positive link between 8-OHdG levels and serum Cr, Mn, Cu, and Pb levels (Tokaç et al, 2020).…”

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“…When taken in large quantities, the copper shows toxic properties for metabolism. The most common of these is oxidative stress and a neurodegenerative Wilson's disease [24], [25]. In this respect, determining the amount of copper in domestic water has been essential.…”