Oxidized lipoproteins increase reactive oxygen species formation in microglia and astrocyte cell lines

Keller, Jeffrey N.; Hanni, Keith B.; Gabbita, S. Prasad; Friebe, Veronica; Mattson, Mark P.; Kindy, Mark S.

doi:10.1016/s0006-8993(99)01272-x

Cited by 53 publications

(31 citation statements)

References 43 publications

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“…Reactive oxygen species (ROS) is considered to be a causal factor in the inflammation responses induced by a variety of stimuli including LPS (Keller et al, 1999;Wang and Joseph, 1999). Cells maintain a balanced cellular redox state by using a complex ROS regulating network which is composed of antioxidant enzymes such as superoxide dismutase (SOD), catalase and glutathione peroxidase, and low molecular mass antioxidants such ascorbic acid, glutathione and tocopherols (Mates, 2000).…”

Section: Introductionmentioning

confidence: 99%

Differential regulation of inducible nitric oxide synthase and cyclooxygenase-2 expression by superoxide dismutase in lipopolysaccharide stimulated RAW 264.7 cells

Lee

Song

et al. 2009

Exp Mol Med

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Inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) have been known to be involved in various pathophysiological processes such as inflammation. This study was performed to determine the regulatory function of superoxide dismutase (SOD) on the LPS-induced expression of iNOS, and COX-2 in RAW 264.7 cells. When a cell-permeable SOD, Tat-SOD, was added to the culture medium of RAW 264.7 cells, it rapidly entered the cells in a dose-dependent manner. Treatment of RAW 264.7 cells with Tat-SOD led to decrease in LPS-induced ROS generation. Pretreatment with Tat-SOD significantly inhibited LPS-induced expression of iNOS and NO production but had no effect on the expression of COX-2 and PGE2 production in RAW 264.7 cells. Tat-SOD inhibited LPS-induced NF-κB DNA binding activity, IκBα degradation and activation of MAP kinases. These data suggest that SOD differentially regulate expression of iNOS and COX-2 in LPS-stimulated RAW 264.7 cells.

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Section: Introductionmentioning

confidence: 99%

Differential regulation of inducible nitric oxide synthase and cyclooxygenase-2 expression by superoxide dismutase in lipopolysaccharide stimulated RAW 264.7 cells

Lee

Song

et al. 2009

Exp Mol Med

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“…Dildar et al (2010) also found that plasma ox-LDL was not related to plasma 3-NT level in AD patients. In contrast, Sinem et al (2010) found that plasma ox-LDL in AD patients was positively related to plasma 3-NT level, which may be because ox-LDL can increase oxidation activity, promote the proliferation of astrocyte cells, microglia cells, and inflammatory cytokines, which in turn exacerbate oxidative stress and stress-related nitration (Keller et al, 1999(Keller et al, , 2000Hsiai et al, 2007).…”

Section: Discussionmentioning

confidence: 93%

Expression and significance of plasma 3-NT and ox-LDL in patients with Alzheimer's disease

Zhi-gang¹,

Zhou²,

Peng³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. To examine the expression and clinical significance of plasma 3-nitrotyrosine (3-NT) and oxidized low-density lipoprotein (ox-LDL) levels in patients with Alzheimer's disease (AD), we examined 48 AD patients and 37 healthy control subjects. The Mini-Mental State Examination, Activities of Daily Living Scale, and Hachinski Ischemic Scale were examined in all subjects. AD patients were classified using the Global Deterioration Scale. The concentrations of plasma 3-NT and ox-LDL were detected using an enzyme-linked immunosorbent assay. We found that the plasma 3-NT concentration in the AD group (119.46 ± 21.82 nM) was significantly higher than that in the control group (55.09 ± 9.63 nM) (P < 0.05). Spearman analysis showed that plasma 3-NT level was negatively associated with the Mini-Mental State Examination results of AD patients. Plasma ox-LDL level in the AD group (112.25 ± 17.81 mg/L) was significantly higher than that in the control group (47.46 ± 10.04 mg/L) (P < 0.05). Spearman analysis showed that plasma ox-LDL level was positively correlated with AD severity in AD patients. However, plasma 3-NT level in the AD group was not associated with plasma ox-LDL level. Therefore, plasma 3-NT 8429©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (4): 8428-8435 (2014) NT and ox-LDL in Alzheimer's disease and ox-LDL levels in AD patients were significantly increased, which may be related to the degree of AD severity in AD patients.

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“…Since ROS plays a major role in a variety of neurodegenerative and neuroinflammatory diseases such as Alzheimer's disease (Keller et al, 1999;Wang and Joseph, 1999), there have been considerable efforts in the use of anti-oxidant enzymes in protection against ROS-mediated diseases (Greenwald, 1990). Recombinant enzymes such as SOD and catalase have been used to protect against oxidative stresses.…”

Section: Discussionmentioning

confidence: 99%

Suppression of HIV-1 Tat-induced monocyte adhesiveness by a cell-permeable superoxide dismutase in astrocytes

Song

Ju²,

Lee³

et al. 2007

Exp Mol Med

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Oxidized lipoproteins increase reactive oxygen species formation in microglia and astrocyte cell lines

Cited by 53 publications

References 43 publications

Differential regulation of inducible nitric oxide synthase and cyclooxygenase-2 expression by superoxide dismutase in lipopolysaccharide stimulated RAW 264.7 cells

Differential regulation of inducible nitric oxide synthase and cyclooxygenase-2 expression by superoxide dismutase in lipopolysaccharide stimulated RAW 264.7 cells

Expression and significance of plasma 3-NT and ox-LDL in patients with Alzheimer's disease

Suppression of HIV-1 Tat-induced monocyte adhesiveness by a cell-permeable superoxide dismutase in astrocytes

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