Oxidovanadium(IV) sulfate-induced glucose uptake in HepG2 cells through IR/Akt pathway and hydroxyl radicals

Zhao, Qian; Chen, Deliang; Liu, Pingsheng; Wei, Taotao; Zhang, Fang; Ding, Wenjun

doi:10.1016/j.jinorgbio.2015.05.005

Cited by 14 publications

(5 citation statements)

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“…However, in our control experiments the AKT signal in HEPG2 cells has not been activated (Figure 5c), suggesting that AKT phosphorylation by MetV, MetfDeca and NaDeca can be attributed to the activation of PTB-1B by orthovanadate (V 1 ) [32]. On the other hand, for NaDeca compound, the tetramer species is formed at the same speed than V 1 (Table 1), V 4 could be the one that is reduced [55] and the vanadium (IV) species VO 2+ is activating the AKT pathway in a PI3K-dependent manner by ROS, like in the case of VOSO 4 that exhibited a 17-fold increase in the phosphorylation of AKT in HEPG2 cells at 25 µM concentration [56]. In 2015 Levina and coworkers performed a speciation study by XANES spectroscopy, where for 1 mM of orthovanadate in HEPG2 cells with DMEM medium after 24 h, 50% of the initial vanadium was found as tetrahedral species of V 5+ (V 1 , V 2 , V 4 and V 5 are tetrahedral), 30% as V 4+ moieties with a coordination number of six and 20% as V 4+ with a coordination number of five [19], this study supports our observation that after 24 h not more decavanadate species are present in solution.…”

Section: Proteins Expressionmentioning

confidence: 95%

Kinetic Studies of Sodium and Metforminium Decavanadates Decomposition and In Vitro Cytotoxicity and Insulin- Like Activity

et al. 2020

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The kinetics of the decomposition of 0.5 and 1.0 mM sodium decavanadate (NaDeca) and metforminium decavanadate (MetfDeca) solutions were studied by 51V NMR in Dulbecco’s modified Eagle’s medium (DMEM) medium (pH 7.4) at 25 °C. The results showed that decomposition products are orthovanadate [H2VO4]− (V1) and metavanadate species like [H2V2O7]2− (V2), [V4O12]4− (V4) and [V5O15]5− (V5) for both compounds. The calculated half-life times of the decomposition reaction were 9 and 11 h for NaDeca and MetfDeca, respectively, at 1 mM concentration. The hydrolysis products that presented the highest rate constants were V1 and V4 for both compounds. Cytotoxic activity studies using non-tumorigenic HEK293 cell line and human liver cancer HEPG2 cells showed that decavanadates compounds exhibit selectivity action toward HEPG2 cells after 24 h. The effect of vanadium compounds (8–30 μM concentration) on the protein expression of AKT and AMPK were investigated in HEPG2 cell lines, showing that NaDeca and MetfDeca compounds exhibit a dose-dependence increase in phosphorylated AKT. Additionally, NaDeca at 30 µM concentration stimulated the glucose cell uptake moderately (62%) in 3T3-L1 adipocytes. Finally, an insulin release assay in βTC-6 cells (30 µM concentration) showed that sodium orthovanadate (MetV) and MetfDeca enhanced insulin release by 0.7 and 1-fold, respectively.

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Section: Proteins Expressionmentioning

confidence: 95%

Kinetic Studies of Sodium and Metforminium Decavanadates Decomposition and In Vitro Cytotoxicity and Insulin- Like Activity

et al. 2020

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“…For instance, oxidovanadium(IV/V) chlorodipicolinate species improve hyperglicemia and glucose intolerance in streptozotocin (STZ)-induced diabetic rats [7]. Also, VOSO 4 was shown to improve glucose uptake in human hepatocellular carcinoma (HepG2) cells via a signaling pathway activated by generation of the  OH radical, which has a key role in the insulin receptor/protein kinase B phosphorylation system [8]. In this context, HepG2 cells are considered an alternative for in vitro investigation of insulin-dependent pathways, due to the conservation of characteristics common to hepatocytes and preservation of the glucose transporter (GLUT) activity [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

“…In vitro studies of insulin-mimetic action quantified by 2-NBDG uptake after 24 h of incubation in the presence of vanadium complexes 2'-((ethane-1,2-diylbis(azanediyl))bis(methylene))bis(4-(phenyldiazenyl)phenol)), and otp = pyridoxylidenetryptamine. # This Work.It was recently demonstrated that VOSO 4 promotes the generation of the  OH radical in HepG2 cells, increasing intracellular levels of reactive oxygen species (ROS) in a dosedependent manner; this is essential for triggering the signaling response of insulin[8]. In the context of the present work, the promising results obtained for I open the way for the investigation of the active vanadium species formed in DMEM (and observed by X-band EPR), both in terms of chemical nature and general occurrence, particularly when starting from other water-soluble vanadium(IV) complexes.…”

mentioning

confidence: 99%

An oxalate-bridged oxidovanadium(IV) binuclear complex that improves the in vitro cell uptake of a fluorescent glucose analog

et al. 2021

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“…In fact, this molecular cascade has action on other enzymes, such as PI3K-PK β /Akt-mTOR, NF- κβ , and MEK1/2-ERK activation. These messengers are related to GLUT transporter insertion into the plasma membrane, which results in increased glucose transport into the cells and subsequently decreased blood glucose and insulin [21, 23, 47, 48]. Gluconeogenesis and hepatic glucose output are decreased in treatment with vanadium; consequently, this compound could diminish the loss of muscle protein that occurs in T2DM patients [16].…”

Section: Discussionmentioning

confidence: 99%

Vanadyl Sulfate Effects on Systemic Profiles of Metabolic Syndrome in Old Rats with Fructose-Induced Obesity

Ortega-Pacheco

Jiménez-Pérez

Serafín‐López

et al. 2018

International Journal of Endocrinology

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Background Currently, energy obtained from hypercaloric diets has been part of the obesity and type 2 diabetes mellitus (T2DM) epidemics from childhood to old age. Treatment alternatives have been sought from plants, minerals, and trace elements with metabolic effects. Vanadyl sulfate (VS) has been investigated as a hypoglycemic compound in animal and human studies showing effective insulin-mimetic properties. This characteristic encompasses several molecules that have beneficial pleiotropic effects. The aim was to determine the antiobesity, hypoglycemic, and hypolipidemic effects of VS on fructose-induced metabolic syndrome in aged rats. Material and Methods Five groups of male Wistar rats were made, each with six rats: two groups with normal diet (ND) and three with high-fructose diet (HFD). The first ND group was treated with saline solution (SS), the second with VS; treatment for HFD groups was in the first group with SS, second with VS, and third with metformin. Weight, body mass index (BMI), blood glucose, and lipidic profile were measured; water, food, fructose and energy consumption were also determined. All parameters were compared among groups. Results and Discussion Although obese rats treated with VS presented anorexia, oligodipsia, and a marked weight loss in the first two weeks. They recovered food and water intake in the third week with a slow recovery of some weight weeks later. VS normalized blood glucose level and decreased triglyceride and insulin levels in obese rats. These results suggest that vanadyl sulfate shows antiobesity, hypoglycemic, and hypolipidemic properties in old obese rats and could be useful as an alternative, additional, and potent preventive treatment for obesity and T2DM control in elderly obese and poorly controlled diabetic patients. Conclusion VS could play an important role in the treatment of metabolic syndrome, contributing to a decrease in obesity and T2DM, through different ways, such as euglycemia, satiety, weight loss, and lipid profile optimization, among others. However, more research is needed to confirm this suggestion.

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Oxidovanadium(IV) sulfate-induced glucose uptake in HepG2 cells through IR/Akt pathway and hydroxyl radicals

Cited by 14 publications

References 54 publications

Kinetic Studies of Sodium and Metforminium Decavanadates Decomposition and In Vitro Cytotoxicity and Insulin- Like Activity

Kinetic Studies of Sodium and Metforminium Decavanadates Decomposition and In Vitro Cytotoxicity and Insulin- Like Activity

An oxalate-bridged oxidovanadium(IV) binuclear complex that improves the in vitro cell uptake of a fluorescent glucose analog

Vanadyl Sulfate Effects on Systemic Profiles of Metabolic Syndrome in Old Rats with Fructose-Induced Obesity

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