Oxime-based 19-nortestosterone–pheophorbide<i>a</i>conjugate: bimodal controlled release concept for PDT

Pavlíčková, Vladimíra Svobodová; Jurášek, Michal; Rimpelová, Silvie; Záruba, Кamil; Sedlák, David; Šimková, M; Kodr, David; Staňková, Eliška; Fähnrich, Jan; Rottnerová, Zdeňka; Bartůněk, Petr; Lapčı́k, Oldřich; Drašar, Pavel; Ruml, Tomáš

doi:10.1039/c9tb01301f

Cited by 10 publications

(13 citation statements)

References 98 publications

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“…PEGylated derivatives have higher hydrophilicity, can significantly enhance phototoxicity, and can be used in the PDT of cervical cancer, prostate cancer, pancreatic cancer, and breast cancer [47]. Similarly, the phototoxicity effect of NT-pheophorbide (NT-Pba), a conjugate composed of red luminescent pheophorbide-a and nandrolone (NT), is more pronounced than the original pheophorbide-a [49]. In addition, methylene blue (MB) has low toxicity and can be activated at a wavelength of 666 nm; the yield of singlet oxygen is high [50,51].…”

Section: First-and Second-generation Photosensitizersmentioning

confidence: 99%

Viral Nanoparticle System: An Effective Platform for Photodynamic Therapy

Lin

Liu

Han

et al. 2021

IJMS

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Photodynamic therapy (PDT) is a promising therapy due to its efficiency and accuracy. The photosensitizer is delivered to the target lesion and locally activated. Viral nanoparticles (VNPs) have been explored as delivery vehicles for PDT in recent years because of their favorable properties, including simple manufacture and good safety profile. They have great potential as drug delivery carriers in medicine. Here, we review the development of PDT photosensitizers and discuss applications of VNP-mediated photodynamic therapies and the performance of VNPs in the treatment of tumor cells and antimicrobial therapy. Furthermore, future perspectives are discussed for further developing novel viral nanocarriers or improving existing viral vectors.

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Section: First-and Second-generation Photosensitizersmentioning

confidence: 99%

Viral Nanoparticle System: An Effective Platform for Photodynamic Therapy

Lin

Liu

Han

et al. 2021

IJMS

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“…Relative spectral emission intensity of the illuminationg source was measured using spectrofluorometer Fluoromax 2 (Horiba Scientific, Horiba Ltd., Kyoto, Japan). The lamp illuminated a plate made of barium sulfate positioned on front surface accessory of the spectrofluorometer and its spectrum was recorded as reported by Pavlíčková et al [5] Emission correction function supplied by manufacturer was applied to the measured spectra. The same correction factor was used to correct spectra of quinine sulfate solution in sulfuric acid and results corresponded well with standard spectra by Velapoldi and Tønnesen [84].…”

Section: Data Measurementmentioning

confidence: 99%

“…Data were evaluated using MS Excel 2010 (Microsoft, Redmond, WA, USA) with a procedure described in detail by Pavlíčková et al [5] Briefly, from concentrations and a spectrum of a tested compound, its absorbance A(λ,t) in solutions was estimated, and then, relative exposure I A (t) for solution illuminated for time t was calculated as…”

Section: Data Evaluationmentioning

confidence: 99%

“…Various modified substructures derived from their basic core have been discovered within the plant kingdom [1][2][3][4]. Owing to their strong absorption between 650-700 nm, wavelengths that penetrate tissue effectively, chlorins have been investigated as photosensitizers (PSs) for use in the photodynamic therapy (PDT) of cancerous and noncancerous diseases [5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

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PEGylated Purpurin 18 with Improved Solubility: Potent Compounds for Photodynamic Therapy of Cancer

et al. 2019

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Purpurin 18 derivatives with a polyethylene glycol (PEG) linker were synthesized as novel photosensitizers (PSs) with the goal of using them in photodynamic therapy (PDT) for cancer. These compounds, derived from a second-generation PS, exhibit absorption at long wavelengths; considerable singlet oxygen generation and, in contrast to purpurin 18, have higher hydrophilicity due to decreased logP. Together, these properties make them potentially ideal PSs. To verify this, we screened the developed compounds for cell uptake, intracellular localization, antitumor activity and induced cell death type. All of the tested compounds were taken up into cancer cells of various origin and localized in organelles known to be important PDT targets, specifically, mitochondria and the endoplasmic reticulum. The incorporation of a zinc ion and PEGylation significantly enhanced the photosensitizing efficacy, decreasing IC50 (half maximal inhibitory compound concentration) in HeLa cells by up to 170 times compared with the parental purpurin 18. At effective PDT concentrations, the predominant type of induced cell death was apoptosis. Overall, our results show that the PEGylated derivatives presented have significant potential as novel PSs with substantially augmented phototoxicity for application in the PDT of cervical, prostate, pancreatic and breast cancer.

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“…Several authors have made an effort to enhance PDT anti-tumor effectiveness. Many researchers worked on novel photosensitizing agents [4], light sources [5], and also novel ways of photosensitizer targeting [6]. There are also attempts to deliver photosensitizers to tumor cells more effectively using local hyperthermia or creating liposomal forms of classic photosensitizers [7].…”

Section: Introductionmentioning

confidence: 99%

The Assessment of the Combined Treatment of 5-ALA Mediated Photodynamic Therapy and Thalidomide on 4T1 Breast Carcinoma and 2H11 Endothelial Cell Line

et al. 2020

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Photodynamic therapy (PDT) is a low-invasive method of treatment of various diseases, mainly neoplastic conditions. PDT has been experimentally combined with multiple treatment methods. In this study, we tested a combination of 5-aminolevulinic acid (5-ALA) mediated PDT with thalidomide (TMD), which is a drug presently used in the treatment of plasma cell myeloma. TMD and PDT share similar modes of action in neoplastic conditions. Using 4T1 murine breast carcinoma and 2H11 murine endothelial cells lines as an experimental tumor model, we tested 5-ALA-PDT and TMD combination in terms of cytotoxicity, apoptosis, Vascular Endothelial Growth Factor (VEGF) expression, and, in 2H11 cells, migration capabilities by wound healing assay. We have found an enhancement of cytotoxicity in 4T1 cells, whereas, in normal 2H11 cells, this effect was not statistically significant. The addition of TMD decreased the production of VEGF after PDT in 2H11 cell line. Our results reveal enhanced effectiveness of 5-ALA-PDT with TMD treatment compared to 5-ALA-PDT or TMD treatment alone. The addition of TMD may be a promising proceeding of the anti-tumor effect of PDT by decreasing the VEGF concentration in the culture medium. Further studies, including testing on different cell lines, are needed to confirm this assumption.

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Oxime-based 19-nortestosterone–pheophorbideaconjugate: bimodal controlled release concept for PDT

Abstract: Nandrolone–pheophorbide a fluorescent conjugate exhibiting promising potential in targeting of androgen receptor-positive cancer cells and their destruction by photodynamic therapy.

Cited by 10 publications

References 98 publications

Viral Nanoparticle System: An Effective Platform for Photodynamic Therapy

Viral Nanoparticle System: An Effective Platform for Photodynamic Therapy

PEGylated Purpurin 18 with Improved Solubility: Potent Compounds for Photodynamic Therapy of Cancer

The Assessment of the Combined Treatment of 5-ALA Mediated Photodynamic Therapy and Thalidomide on 4T1 Breast Carcinoma and 2H11 Endothelial Cell Line

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